MADS-box gene family encodes a large number and variety of transcription regulators in plants. In this study, a cDNA, GhMADS9, encoding a typical MADS protein with 230 amino acids was isolated from cotton flower cDNA ...MADS-box gene family encodes a large number and variety of transcription regulators in plants. In this study, a cDNA, GhMADS9, encoding a typical MADS protein with 230 amino acids was isolated from cotton flower cDNA library. Subsequently, a 1,623 bp genomic DNA fragment of GhMADS9 gene was isolated in cotton by PCR. Compared with its cDNA sequence, six introns were found in GhMADS9 gene. Fluorescent microscopy indicated that GhMADS9 protein localized in the nucleus. Transactivation activity assay in yeast cells revealed that GhMADS9 protein did not show transcriptional activation. Quantitative RT-PCR analysis showed that GhMADS9 was specially expressed in cotton anthers. Further in situ hybridization analysis demonstrated that strong expression of GhMADS9 gene was detected in developing pollens, but no or weak signals were found in the other anther tissues. Furthermore, GhMADS9 expression was dramatically up-regulated in anthers with abscisic acid (ABA) treatment, whereas its activity was down-regulated when treated by gibberellin (GA3). Collectively, our results suggest that GhMADS9 is a transcription factor and might be involved in cotton anther/pollen development and in response to ABA and GA3 signaling.展开更多
大葱(Allium fistulo sum L.)雄性可育系的减数分裂期的小孢子母细胞及四分体,有厚度不均的胼胝质壁。成熟花粉为二胞花粉,生殖细胞长,具PAS正反应的细胞壁。绒毡层兼有腺质及周缘质团型特点。自小孢子期始,绒毡层细胞的细胞质内含有黄...大葱(Allium fistulo sum L.)雄性可育系的减数分裂期的小孢子母细胞及四分体,有厚度不均的胼胝质壁。成熟花粉为二胞花粉,生殖细胞长,具PAS正反应的细胞壁。绒毡层兼有腺质及周缘质团型特点。自小孢子期始,绒毡层细胞的细胞质内含有黄色油脂颗粒或团块,在二胞花粉中期,绒毡层退化。不育系的小孢子母细胞的减数分裂正常,花粉败育发生在具液泡的小孢子期。败育Ⅰ型的小孢子原生质体收缩,并逐渐解体,同时在细胞质中积累油滴,存留于空花粉中。绒毡层细胞,在小孢子及小孢子败育初期,与可育系者相似,唯含油脂颗粒或团块比可育系者少,个别花药的绒毡层细胞含小液泡特多。小孢子败育后期,绒毡层细胞显示出:(1)细胞扁化,原生质体解体退化中;(2)细胞体积扩大,原生质体收缩;(3)绒毡层破毁,中层扩大。败育Ⅱ型的小孢子细胞质先于细胞核解体。两种败育类型的花药,其药室内壁细胞均具发达的条纹加厚壁。本观察难以说明绒毡层与花粉败育的因果关系。展开更多
基金supported by the National Natural Sciences Foundation of China (No. 30871317)the project from Ministry of Agriculture of China for transgenic research (No. 2009ZX08009-117B)
文摘MADS-box gene family encodes a large number and variety of transcription regulators in plants. In this study, a cDNA, GhMADS9, encoding a typical MADS protein with 230 amino acids was isolated from cotton flower cDNA library. Subsequently, a 1,623 bp genomic DNA fragment of GhMADS9 gene was isolated in cotton by PCR. Compared with its cDNA sequence, six introns were found in GhMADS9 gene. Fluorescent microscopy indicated that GhMADS9 protein localized in the nucleus. Transactivation activity assay in yeast cells revealed that GhMADS9 protein did not show transcriptional activation. Quantitative RT-PCR analysis showed that GhMADS9 was specially expressed in cotton anthers. Further in situ hybridization analysis demonstrated that strong expression of GhMADS9 gene was detected in developing pollens, but no or weak signals were found in the other anther tissues. Furthermore, GhMADS9 expression was dramatically up-regulated in anthers with abscisic acid (ABA) treatment, whereas its activity was down-regulated when treated by gibberellin (GA3). Collectively, our results suggest that GhMADS9 is a transcription factor and might be involved in cotton anther/pollen development and in response to ABA and GA3 signaling.
文摘大葱(Allium fistulo sum L.)雄性可育系的减数分裂期的小孢子母细胞及四分体,有厚度不均的胼胝质壁。成熟花粉为二胞花粉,生殖细胞长,具PAS正反应的细胞壁。绒毡层兼有腺质及周缘质团型特点。自小孢子期始,绒毡层细胞的细胞质内含有黄色油脂颗粒或团块,在二胞花粉中期,绒毡层退化。不育系的小孢子母细胞的减数分裂正常,花粉败育发生在具液泡的小孢子期。败育Ⅰ型的小孢子原生质体收缩,并逐渐解体,同时在细胞质中积累油滴,存留于空花粉中。绒毡层细胞,在小孢子及小孢子败育初期,与可育系者相似,唯含油脂颗粒或团块比可育系者少,个别花药的绒毡层细胞含小液泡特多。小孢子败育后期,绒毡层细胞显示出:(1)细胞扁化,原生质体解体退化中;(2)细胞体积扩大,原生质体收缩;(3)绒毡层破毁,中层扩大。败育Ⅱ型的小孢子细胞质先于细胞核解体。两种败育类型的花药,其药室内壁细胞均具发达的条纹加厚壁。本观察难以说明绒毡层与花粉败育的因果关系。
