This study set out to look into the palyno-morphological characteristics of specific allergenic species from diverse plant families in Samarkand, Uzbekistan. Six different species i.e. Betula pendula, Fraxinus pennsyl...This study set out to look into the palyno-morphological characteristics of specific allergenic species from diverse plant families in Samarkand, Uzbekistan. Six different species i.e. Betula pendula, Fraxinus pennsylvanica, Magnolia × soulangeana, Pinus brutia var. eldarica, Populus alba, Quercus robur were collected, pressed, identified, and then examined under a microscope. Under light microscope, both quantitative and qualitative characteristics of the pollen grains were recorded, including pollen type, pollen size, pollen shape (polar and equatorial view), P/E ratio, mesocolpium distance, exine thickness, colpi type, colpi length, and width. It was found that a warm spring in Uzbekistan causes an early commencement of the vegetative and dusting phases, in contrast to the climatic circumstances of the researched entities’ motherland (cold spring). Studies in aeropalinology and morphology enable the resolution of numerous environmental issues. The findings highlighted the importance of pollen morphology as an identifying aid and showed how the tested species’ palynological characteristics varied. In order to promote future phylogenetic description of the Uzbekistan flora, the current study may help us better understand the palynomorphological traits of native plants. This essay only briefly and inadequately addresses these issues. The information gathered will be used to make an atlas of allergic plants in Samarkand and an electronic warning system for the general public about the spread of pollen from the city’s allergenic plants in the future.展开更多
Cow's milk allergy is mainly observed in infants and young children. Most allergic reactions affect the skin, followed by the gastrointestinal and respiratory systems. Conventional diagnosis is based on positive a...Cow's milk allergy is mainly observed in infants and young children. Most allergic reactions affect the skin, followed by the gastrointestinal and respiratory systems. Conventional diagnosis is based on positive allergy studies and evaluation of parameters including IgE and IgG1 levels, acute allergic skin response and anaphylactic shock reactions. We developed a cell membrane chromatographic(CMC)method based on human mast cells(HMC-1) for screening potential allergens in infant formula milk powders(IFMP). HMC-1 cell membranes were extracted and mixed with silica to prepare cell membrane chromatography columns(10 mm ? 2 mm i.d., 5 mm). Under the conditions of 0.2 mL/min flow rate and214 nm detection wavelength, human breast milk showed no retention. However, IFMP showed clear retention. The retained fractions were collected and analyzed through matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS). Four major milk proteins, i.e., α-casein, β-casein, α-lactalbumin, and β-lactoglobulin A, were identified. Furthermore, these proteins and β-lactoglobulin B showed clear retention on HMC-1/CMC columns. To test the degranulation effects of the five proteins, histamine and β-hexosaminidase release assays were carried out. All five proteins induced HMC-1 cells to release histamine and β-hexosaminidase. Also, we established a reversed phase liquid chromatographic(RPLC) method for the determination of the five proteins in IFMP and the results showed that 90% proteins in IFMP were α-casein and β-casein. We concluded that cow's milk proteins may be potential allergens and caseins cause more β-casein allergic risk than other proteins. This conclusion was consistent with other studies.展开更多
A gold nanoparticle-based label-free colorimetric assay was developed to detect the shrimp allergenic protein tropomyosin(TM),an important biomarker responsible for severe clinical reactivity to shellfish.In a gold na...A gold nanoparticle-based label-free colorimetric assay was developed to detect the shrimp allergenic protein tropomyosin(TM),an important biomarker responsible for severe clinical reactivity to shellfish.In a gold nanoparticles(AuNPs)-tropomyosin-binding aptamer(TMBA)complex,the aptamer adsorbs onto the surface of AuNPs and dissociates in the presence of TM.In addition,AuNPs tend to aggregate in the presence of ionic salt,revealing a color change(i.e.,wine-red to purple/blue)with a shift in the maximum absorption peak from 520 nm.In the presence of specific binding TM,the aptamer folds into a tertiary structure where it more efficiently stabilizes AuNPs toward the salt-induced aggregation with a hypsochromic shift in the absorption spectra compared to the stabilized AuNPs by aptamer alone.Based on the aggregation and sensitive spectral transformation principle,the AuNPs-based colorimetric aptasensor was successfully applied to detect TM with a range of 10-200 nmol/L and a low detection limit of 40 nmol/L in water samples.The reliability,selectivity,and sensitivity of the aptasensor was then tested with food samples spiked with TM.The observed detection limit was as low as 70 nmol/L in shrimp,90 nmol/L in tofu,and 80 nmol/L in eggs,respectively.We anticipate the proposed AuNPs-based colorimetric aptasensor assay possesses a high potential for the easy and efficient visual colorimetric detection of TM.展开更多
Background: Wheat is a potent allergen source and is one of the causes of baker’s asthma and food allergy. The best strategy for managing food hypersensitivity involves strict avoidance of the trigger. However, wheat...Background: Wheat is a potent allergen source and is one of the causes of baker’s asthma and food allergy. The best strategy for managing food hypersensitivity involves strict avoidance of the trigger. However, wheat is quite difficult to avoid. Several alternative strategies for the treatment of food allergy are under study. Spelt is a possible hypoallergenic crop that may be tried in patients with wheat allergy. Methods: We have evaluated the allergenic IgE hypersensitivity mediated by spelt in wheat allergic patients. Overall, 66 patients who suffered from baker’s asthma or food allergy (45 males and 21 females, mean age 28.6 ± 12.9 years) were included. We have also compared its reactivity with standard- ized extracts from wheat and with purified non-specific lipid transfer proteins from wheat (Tri a 14) and from peach (Pru p 3). Immunodetection with spelt and common bread wheat extracts (Triticum aestivum, cultivar Astral) was per- formed. Fresh wheat and spelt grain extracts were used both for oral and bronchial challenge and skin tests. Specific IgE detection to different cereals was performed using the Immuno CAP System (Phadia, Uppsala, Sweden). The bronchial challenge was positive with wheat Astral in 44 (67%) patients, all of them suffered from asthma. Thirteen (29.54%) of these 44 patients had negative the challenge with spelt. The oral challenge with wheat Astral was positive in 22 (33%) patients with wheat food allergy, and the same test was positive in only in 6 of them with spelt (27.3%). The diagnostic yield (sensitivity, specificity and predictive values) of routine tests in determining spelt allergy by specific positive challenge responses was determined. Prick tests for spelt versus positive challenge tests had a good sensitivity (94%, 86.5 - 99.4;95%CI) and specificity (86%, 84 - 90;95% CI) for the diagnosis of spelt allergy. Immunodetection detected minor differences among different extracts. Conclusion: In summary, the prick test and bronchial and oral challenges both efficiently detec展开更多
Mature and immature roasted peanuts are reportedly different in the level of Maillard reaction adducts (MRA) and IgE binding (i.e., allergenic capacity). Heating and sugar-protein interaction are the cause for the dif...Mature and immature roasted peanuts are reportedly different in the level of Maillard reaction adducts (MRA) and IgE binding (i.e., allergenic capacity). Heating and sugar-protein interaction are the cause for the difference. The objective of this study was to determine if mature and immature raw peanuts (not roasted) are also different through treatment with a reducing sugar such as ribose, glucose or fructose at a mild temperature. Extracts from mature and immature raw peanuts were treated with individual sugars at 37?C and 50?C, respectively, for 0 - 10 days, and then assayed for MRA with nitroblue tetrazolium (NBT) in a time-course manner for 60 min. IgE binding was determined in an enzyme- linked immunosorbent assay (ELISA), using a pooled plasma from peanut-allergic individuals. Of the sugars tested, only ribose produced a big difference or a unique curve pattern in MRA between treated mature and immature peanuts. The unique curve pattern was more pronounced at 50?C (day 5 - 10) than at 37?C. IgE binding under this condition increased, but only with the ribose-treated mature raw peanut. It was concluded that mature and immature raw peanuts were different in MRA and IgE binding when treated with ribose only, and that under such a condition, mature and immature raw peanuts could be identified.展开更多
By classifying non-hydrogen atoms of organic compounds,parametric dyeing,and establishing the relationship between non-hydrogen atoms,new structure descriptors were obtained.The structures of 48 common allergenic frag...By classifying non-hydrogen atoms of organic compounds,parametric dyeing,and establishing the relationship between non-hydrogen atoms,new structure descriptors were obtained.The structures of 48 common allergenic fragrance organic compounds were parametrically characterized.The multiple linear regression(MLR)and partial least-squares regression(PLS)methods were used to build two models of relationship between the compound structure and chromatographic retention time.The stability of the models was evaluated by the"leave-one-out"cross test,and the predictive ability of the models was tested using an external sample set.The correlation coefficients(R2)of the two models are 0.9791 and 0.9744,those(R(CV)~2)of the cross test are 0.8542 and 0.7464,and those(R(test)~2)of the external prediction are 0.9802 and 0.9367,indicating that the models built have good fitting ability,stability and external forecasting capabilities.The structural factors affecting the chromatographic retention time of the compounds were analyzed.The results show that the compound with more secondary carbon atoms may have larger chromatographic retention time(tR)value.This paper has certain reference value for the study on the relationship between the structures and properties of allergenic fragrance organic compounds.展开更多
During three years lasting studies on the territory of the city of Novi Sad, terrain studies of Ambrosia artemisiifolia L. distribution as well as mapping were performed on regulated and disordered green areas and on ...During three years lasting studies on the territory of the city of Novi Sad, terrain studies of Ambrosia artemisiifolia L. distribution as well as mapping were performed on regulated and disordered green areas and on arable areas. For mapping of ragweed distribution, partially modified method of Braun-Blanquet was used, and data processing was perfomed by specially designed program Ambrosia Spot Marker. Monitoring of this species was also done because of the occurrence of retrovegetation after mowing. During vegetation period, this allergenic weed species was controlled by multiple mowing, and rough ruderal sites outside settlements were treated by glyphosate at a rate of 1.2 kg ha^-1 -2.4 kg ha^-1. The study comprehended also monitoring of pollen concentrations in the air by Rotorod pollen sampler spore collector. A. artemisiifolia L. was established in 21 city zones on over 200 localities, on the banks of the river Danube, in Petrovaradin and Sremski Karlovci. Recommended mechanical measures such as mowing, drilling in the phase of germination, and chemical control measures, resulted in significant reduction ofA. artemisiifolia L. In the second half of the August 2009 the highest number of pollen grains was found in the amount of 783 pollen grains per m3 of air.展开更多
Objective To investigate the preliminary clinical outcome of the femur RetroButton combined with glaze allogenic cortical press-fit bolt fixation in ACL reconstruction.Methods A series of 10 patients suffered from ACL...Objective To investigate the preliminary clinical outcome of the femur RetroButton combined with glaze allogenic cortical press-fit bolt fixation in ACL reconstruction.Methods A series of 10 patients suffered from ACL ruptures展开更多
文摘This study set out to look into the palyno-morphological characteristics of specific allergenic species from diverse plant families in Samarkand, Uzbekistan. Six different species i.e. Betula pendula, Fraxinus pennsylvanica, Magnolia × soulangeana, Pinus brutia var. eldarica, Populus alba, Quercus robur were collected, pressed, identified, and then examined under a microscope. Under light microscope, both quantitative and qualitative characteristics of the pollen grains were recorded, including pollen type, pollen size, pollen shape (polar and equatorial view), P/E ratio, mesocolpium distance, exine thickness, colpi type, colpi length, and width. It was found that a warm spring in Uzbekistan causes an early commencement of the vegetative and dusting phases, in contrast to the climatic circumstances of the researched entities’ motherland (cold spring). Studies in aeropalinology and morphology enable the resolution of numerous environmental issues. The findings highlighted the importance of pollen morphology as an identifying aid and showed how the tested species’ palynological characteristics varied. In order to promote future phylogenetic description of the Uzbekistan flora, the current study may help us better understand the palynomorphological traits of native plants. This essay only briefly and inadequately addresses these issues. The information gathered will be used to make an atlas of allergic plants in Samarkand and an electronic warning system for the general public about the spread of pollen from the city’s allergenic plants in the future.
基金supported by the National Natural Science Foundation of China (No: 81230079, 81102414, 81227802)the Natural Science Basic Research Plan in Shaanxi Province of China (Program No. 2017JQ8024)
文摘Cow's milk allergy is mainly observed in infants and young children. Most allergic reactions affect the skin, followed by the gastrointestinal and respiratory systems. Conventional diagnosis is based on positive allergy studies and evaluation of parameters including IgE and IgG1 levels, acute allergic skin response and anaphylactic shock reactions. We developed a cell membrane chromatographic(CMC)method based on human mast cells(HMC-1) for screening potential allergens in infant formula milk powders(IFMP). HMC-1 cell membranes were extracted and mixed with silica to prepare cell membrane chromatography columns(10 mm ? 2 mm i.d., 5 mm). Under the conditions of 0.2 mL/min flow rate and214 nm detection wavelength, human breast milk showed no retention. However, IFMP showed clear retention. The retained fractions were collected and analyzed through matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS). Four major milk proteins, i.e., α-casein, β-casein, α-lactalbumin, and β-lactoglobulin A, were identified. Furthermore, these proteins and β-lactoglobulin B showed clear retention on HMC-1/CMC columns. To test the degranulation effects of the five proteins, histamine and β-hexosaminidase release assays were carried out. All five proteins induced HMC-1 cells to release histamine and β-hexosaminidase. Also, we established a reversed phase liquid chromatographic(RPLC) method for the determination of the five proteins in IFMP and the results showed that 90% proteins in IFMP were α-casein and β-casein. We concluded that cow's milk proteins may be potential allergens and caseins cause more β-casein allergic risk than other proteins. This conclusion was consistent with other studies.
基金This work was supported by National Natural Science Foundation of China(31771892).
文摘A gold nanoparticle-based label-free colorimetric assay was developed to detect the shrimp allergenic protein tropomyosin(TM),an important biomarker responsible for severe clinical reactivity to shellfish.In a gold nanoparticles(AuNPs)-tropomyosin-binding aptamer(TMBA)complex,the aptamer adsorbs onto the surface of AuNPs and dissociates in the presence of TM.In addition,AuNPs tend to aggregate in the presence of ionic salt,revealing a color change(i.e.,wine-red to purple/blue)with a shift in the maximum absorption peak from 520 nm.In the presence of specific binding TM,the aptamer folds into a tertiary structure where it more efficiently stabilizes AuNPs toward the salt-induced aggregation with a hypsochromic shift in the absorption spectra compared to the stabilized AuNPs by aptamer alone.Based on the aggregation and sensitive spectral transformation principle,the AuNPs-based colorimetric aptasensor was successfully applied to detect TM with a range of 10-200 nmol/L and a low detection limit of 40 nmol/L in water samples.The reliability,selectivity,and sensitivity of the aptasensor was then tested with food samples spiked with TM.The observed detection limit was as low as 70 nmol/L in shrimp,90 nmol/L in tofu,and 80 nmol/L in eggs,respectively.We anticipate the proposed AuNPs-based colorimetric aptasensor assay possesses a high potential for the easy and efficient visual colorimetric detection of TM.
文摘Background: Wheat is a potent allergen source and is one of the causes of baker’s asthma and food allergy. The best strategy for managing food hypersensitivity involves strict avoidance of the trigger. However, wheat is quite difficult to avoid. Several alternative strategies for the treatment of food allergy are under study. Spelt is a possible hypoallergenic crop that may be tried in patients with wheat allergy. Methods: We have evaluated the allergenic IgE hypersensitivity mediated by spelt in wheat allergic patients. Overall, 66 patients who suffered from baker’s asthma or food allergy (45 males and 21 females, mean age 28.6 ± 12.9 years) were included. We have also compared its reactivity with standard- ized extracts from wheat and with purified non-specific lipid transfer proteins from wheat (Tri a 14) and from peach (Pru p 3). Immunodetection with spelt and common bread wheat extracts (Triticum aestivum, cultivar Astral) was per- formed. Fresh wheat and spelt grain extracts were used both for oral and bronchial challenge and skin tests. Specific IgE detection to different cereals was performed using the Immuno CAP System (Phadia, Uppsala, Sweden). The bronchial challenge was positive with wheat Astral in 44 (67%) patients, all of them suffered from asthma. Thirteen (29.54%) of these 44 patients had negative the challenge with spelt. The oral challenge with wheat Astral was positive in 22 (33%) patients with wheat food allergy, and the same test was positive in only in 6 of them with spelt (27.3%). The diagnostic yield (sensitivity, specificity and predictive values) of routine tests in determining spelt allergy by specific positive challenge responses was determined. Prick tests for spelt versus positive challenge tests had a good sensitivity (94%, 86.5 - 99.4;95%CI) and specificity (86%, 84 - 90;95% CI) for the diagnosis of spelt allergy. Immunodetection detected minor differences among different extracts. Conclusion: In summary, the prick test and bronchial and oral challenges both efficiently detec
文摘Mature and immature roasted peanuts are reportedly different in the level of Maillard reaction adducts (MRA) and IgE binding (i.e., allergenic capacity). Heating and sugar-protein interaction are the cause for the difference. The objective of this study was to determine if mature and immature raw peanuts (not roasted) are also different through treatment with a reducing sugar such as ribose, glucose or fructose at a mild temperature. Extracts from mature and immature raw peanuts were treated with individual sugars at 37?C and 50?C, respectively, for 0 - 10 days, and then assayed for MRA with nitroblue tetrazolium (NBT) in a time-course manner for 60 min. IgE binding was determined in an enzyme- linked immunosorbent assay (ELISA), using a pooled plasma from peanut-allergic individuals. Of the sugars tested, only ribose produced a big difference or a unique curve pattern in MRA between treated mature and immature peanuts. The unique curve pattern was more pronounced at 50?C (day 5 - 10) than at 37?C. IgE binding under this condition increased, but only with the ribose-treated mature raw peanut. It was concluded that mature and immature raw peanuts were different in MRA and IgE binding when treated with ribose only, and that under such a condition, mature and immature raw peanuts could be identified.
基金the Youth Foundation of Sichuan Provincial Department of Education(18ZB0323)。
文摘By classifying non-hydrogen atoms of organic compounds,parametric dyeing,and establishing the relationship between non-hydrogen atoms,new structure descriptors were obtained.The structures of 48 common allergenic fragrance organic compounds were parametrically characterized.The multiple linear regression(MLR)and partial least-squares regression(PLS)methods were used to build two models of relationship between the compound structure and chromatographic retention time.The stability of the models was evaluated by the"leave-one-out"cross test,and the predictive ability of the models was tested using an external sample set.The correlation coefficients(R2)of the two models are 0.9791 and 0.9744,those(R(CV)~2)of the cross test are 0.8542 and 0.7464,and those(R(test)~2)of the external prediction are 0.9802 and 0.9367,indicating that the models built have good fitting ability,stability and external forecasting capabilities.The structural factors affecting the chromatographic retention time of the compounds were analyzed.The results show that the compound with more secondary carbon atoms may have larger chromatographic retention time(tR)value.This paper has certain reference value for the study on the relationship between the structures and properties of allergenic fragrance organic compounds.
文摘During three years lasting studies on the territory of the city of Novi Sad, terrain studies of Ambrosia artemisiifolia L. distribution as well as mapping were performed on regulated and disordered green areas and on arable areas. For mapping of ragweed distribution, partially modified method of Braun-Blanquet was used, and data processing was perfomed by specially designed program Ambrosia Spot Marker. Monitoring of this species was also done because of the occurrence of retrovegetation after mowing. During vegetation period, this allergenic weed species was controlled by multiple mowing, and rough ruderal sites outside settlements were treated by glyphosate at a rate of 1.2 kg ha^-1 -2.4 kg ha^-1. The study comprehended also monitoring of pollen concentrations in the air by Rotorod pollen sampler spore collector. A. artemisiifolia L. was established in 21 city zones on over 200 localities, on the banks of the river Danube, in Petrovaradin and Sremski Karlovci. Recommended mechanical measures such as mowing, drilling in the phase of germination, and chemical control measures, resulted in significant reduction ofA. artemisiifolia L. In the second half of the August 2009 the highest number of pollen grains was found in the amount of 783 pollen grains per m3 of air.
文摘Objective To investigate the preliminary clinical outcome of the femur RetroButton combined with glaze allogenic cortical press-fit bolt fixation in ACL reconstruction.Methods A series of 10 patients suffered from ACL ruptures
