收集具有代表性的吉林省不同熟期的粳稻种质资源共60份,采用培养皿发芽方法,在盐胁迫(120 m M NaCl,pH=6.98)和碱胁迫(30 m M Na_2CO_3,pH=11.05)条件下培养,并测定发芽势、发芽率、芽长、根长和根数等生长指标,利用隶属度对水稻种质萌...收集具有代表性的吉林省不同熟期的粳稻种质资源共60份,采用培养皿发芽方法,在盐胁迫(120 m M NaCl,pH=6.98)和碱胁迫(30 m M Na_2CO_3,pH=11.05)条件下培养,并测定发芽势、发芽率、芽长、根长和根数等生长指标,利用隶属度对水稻种质萌发期耐盐碱性进行综合评价,为未来盐碱地水稻直播及抗逆育种提供抗逆种质资源。结果表明,水稻萌发期耐盐性和耐碱性呈极显著正相关(r=0.728**),但品种间存在差异。相对芽长和相对根长分别与抗盐隶属度(r_(芽长)=0.862**,r_(根长)=0.781**)和抗碱隶属度(r_(芽长)=0.757**,r_(根长)=0.812**)呈极显著正相关,可作为水稻萌发期耐盐碱性快速筛选的重要指标。利用抗逆隶属度指标将耐盐性和耐碱性划分成高抗(HT)、中抗(MT)、低抗(T)、敏感(S)、中度敏感(MS)和高度敏感(HS)共6个等级,初步筛选出耐盐且耐碱的品种主要有长白21号(HT/HT)、通禾835(HT/HT)、吉玉粳(HT/HT)、长白10号(HT/MT)、吉粘6号(MT/HT)、吉粘10号(MT/MT)、吉粳507(MT/MT)、吉粳509(MT/MT)、吉粳806(MT/MT)和东稻4号(MT/MT);耐盐不耐碱的品种有通禾99(HT/S)、通禾837(MT/S);耐碱不耐盐的品种有吉粳105(MT/S)、吉农大828(MT/S);不耐盐也不耐碱的品种有九稻51号(HS/HS)、吉农大888(HS/HS)和通禾834(HS/MS)等。同时发现,与晚熟或中晚熟品种相比,吉林省中早熟或中熟水稻品种表现出更好的耐盐碱性特征,这对今后水稻耐盐碱育种亲本资源选择具有重要的参考价值。展开更多
Alkaligrass(Puccinellia tenuiflora) is a monocotyledonous halophytic forage grass widely distributed in Northern China. It belongs to the Gramineae family and shares a close phylogenetic relationship with the cereal c...Alkaligrass(Puccinellia tenuiflora) is a monocotyledonous halophytic forage grass widely distributed in Northern China. It belongs to the Gramineae family and shares a close phylogenetic relationship with the cereal crops, wheat and barley. Here, we present a high-quality chromosome-level genome sequence of alkaligrass assembled from Illumina, Pac Bio and 10× Genomics reads combined with genome-wide chromosome conformation capture(Hi-C) data. The ~1.50 Gb assembled alkaligrass genome encodes 38,387 protein-coding genes, and 54.9% of the assembly are transposable elements, with long terminal repeats being the most abundant. Comparative genomic analysis coupled with stress-treated transcriptome profiling uncovers a set of unique saline-and alkaline-responsive genes in alkaligrass. The high-quality genome assembly and the identified stress related genes in alkaligrass provide an important resource for evolutionary genomic studies in Gramineae and facilitate further understanding of molecular mechanisms underlying stress tolerance in monocotyledonous halophytes. The alkaligrass genome data is freely available at http://xhhuanglab.cn/data/alkaligrass.html.展开更多
文摘收集具有代表性的吉林省不同熟期的粳稻种质资源共60份,采用培养皿发芽方法,在盐胁迫(120 m M NaCl,pH=6.98)和碱胁迫(30 m M Na_2CO_3,pH=11.05)条件下培养,并测定发芽势、发芽率、芽长、根长和根数等生长指标,利用隶属度对水稻种质萌发期耐盐碱性进行综合评价,为未来盐碱地水稻直播及抗逆育种提供抗逆种质资源。结果表明,水稻萌发期耐盐性和耐碱性呈极显著正相关(r=0.728**),但品种间存在差异。相对芽长和相对根长分别与抗盐隶属度(r_(芽长)=0.862**,r_(根长)=0.781**)和抗碱隶属度(r_(芽长)=0.757**,r_(根长)=0.812**)呈极显著正相关,可作为水稻萌发期耐盐碱性快速筛选的重要指标。利用抗逆隶属度指标将耐盐性和耐碱性划分成高抗(HT)、中抗(MT)、低抗(T)、敏感(S)、中度敏感(MS)和高度敏感(HS)共6个等级,初步筛选出耐盐且耐碱的品种主要有长白21号(HT/HT)、通禾835(HT/HT)、吉玉粳(HT/HT)、长白10号(HT/MT)、吉粘6号(MT/HT)、吉粘10号(MT/MT)、吉粳507(MT/MT)、吉粳509(MT/MT)、吉粳806(MT/MT)和东稻4号(MT/MT);耐盐不耐碱的品种有通禾99(HT/S)、通禾837(MT/S);耐碱不耐盐的品种有吉粳105(MT/S)、吉农大828(MT/S);不耐盐也不耐碱的品种有九稻51号(HS/HS)、吉农大888(HS/HS)和通禾834(HS/MS)等。同时发现,与晚熟或中晚熟品种相比,吉林省中早熟或中熟水稻品种表现出更好的耐盐碱性特征,这对今后水稻耐盐碱育种亲本资源选择具有重要的参考价值。
基金supported by grants from the National Key Research and Development Program of China(2018YFA090060)the Foundation of Shanghai Science and Technology Committee(17391900600)+1 种基金the Fund of Shanghai Engineering Research Center of Plant Germplasm Resources(17DZ2252700)the Natural Science Foundation of Heilongjiang Province(ZD2019C003)。
文摘Alkaligrass(Puccinellia tenuiflora) is a monocotyledonous halophytic forage grass widely distributed in Northern China. It belongs to the Gramineae family and shares a close phylogenetic relationship with the cereal crops, wheat and barley. Here, we present a high-quality chromosome-level genome sequence of alkaligrass assembled from Illumina, Pac Bio and 10× Genomics reads combined with genome-wide chromosome conformation capture(Hi-C) data. The ~1.50 Gb assembled alkaligrass genome encodes 38,387 protein-coding genes, and 54.9% of the assembly are transposable elements, with long terminal repeats being the most abundant. Comparative genomic analysis coupled with stress-treated transcriptome profiling uncovers a set of unique saline-and alkaline-responsive genes in alkaligrass. The high-quality genome assembly and the identified stress related genes in alkaligrass provide an important resource for evolutionary genomic studies in Gramineae and facilitate further understanding of molecular mechanisms underlying stress tolerance in monocotyledonous halophytes. The alkaligrass genome data is freely available at http://xhhuanglab.cn/data/alkaligrass.html.
