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抗生素抗性基因在养殖废水中的分布与去除 被引量:7
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作者 庄榆佳 赵忆 +1 位作者 苏建强 耿春女 《环境化学》 CAS CSCD 北大核心 2017年第11期2311-2318,共8页
畜禽养殖场作为抗生素抗性基因的一个热点区,其产生的废水中存在大量的抗生素抗性基因,直接排放将会污染接受的水体.本研究以微生物固化曝气技术为核心,构建了一个包含沉淀池、一级处理池、二级处理池和氧化塘的养殖废水处理系统.采用... 畜禽养殖场作为抗生素抗性基因的一个热点区,其产生的废水中存在大量的抗生素抗性基因,直接排放将会污染接受的水体.本研究以微生物固化曝气技术为核心,构建了一个包含沉淀池、一级处理池、二级处理池和氧化塘的养殖废水处理系统.采用高通量荧光定量PCR技术,探究各种抗性基因在进水、各处理池和出水中的种类、多样性和丰度的变化以及可能的影响因素.研究发现:(1)微生物固化曝气技术能有效降低养殖废水中抗性基因的种类和多样性;(2)该技术也能有效去除养殖废水中抗性基因的绝对丰度,去除率高达93.6%;(3)养殖废水出水中抗性基因绝对丰度仍高于自然水体,其直接排放仍有抗性基因污染风险;(4)畜禽养殖废水中TN和土霉素的含量与许多抗生素抗性基因总丰度存在正相关性.通过对TN和土霉素的去除或者控制使用,可以有效降低养殖废水中的抗性基因. 展开更多
关键词 微生物固化曝气技术 高通量荧光定量PCR 抗性基因 多样性和丰度 养殖废水 抗生素
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川西高寒森林溪流好氧不产氧光合细菌多样性 被引量:3
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作者 黄春萍 吴福忠 +3 位作者 杨万勤 岳楷 张川 张健 《中国环境科学》 EI CAS CSSCI CSCD 北大核心 2017年第6期2332-2339,共8页
为了探明好氧不产氧光合菌(AAPB)在川西高寒森林溪流极端环境中的生态分布和群落结构及其环境影响因子,本实验结合同步温度动态和水环境特征监测,采用实时荧光定量PCR和克隆文库的方法,检测了川西高寒森林溪流季节性冻融循环3个关键时期... 为了探明好氧不产氧光合菌(AAPB)在川西高寒森林溪流极端环境中的生态分布和群落结构及其环境影响因子,本实验结合同步温度动态和水环境特征监测,采用实时荧光定量PCR和克隆文库的方法,检测了川西高寒森林溪流季节性冻融循环3个关键时期(冻结前期、深冻期和融化期)AAPB的丰度和多样性变化及其与环境因子之间的相关性.结果表明:AAPB丰度在冻结前期非常低,仅(2.80±0.19)×10~4bp/mL;深冻期迅速增加到(4.65±0.30)×10~4bp/mL;融化期达到(5.79±0.19)×104~bp/mL.系统发育分析显示,冻结前期AAPB类群主要为α-变形菌纲细菌;而深冻期和融化期以β-变形菌纲细菌占据主要地位.相关性分析表明,在季节性冻融循环过程中,叶绿素a、可溶性有机碳和温度是AAPB菌群丰度和多样性的主要影响因子.实验结果表明冬季的川西高寒森林溪流中,AAPB类群的丰度低,但是它们高度动态变化,并且与其他淡水环境中的该类群的同源性低. 展开更多
关键词 好氧不产氧光合菌 丰度和多样性 高寒森林溪流 季节性冻融循环
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长期施用化肥对红壤磷形态、磷酸酶活性及phoD基因丰度和多样性的影响
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作者 黄文晶 姜楠 +5 位作者 陈晓冬 孙楠 徐明岗 陈振华 张玉兰 陈利军 《生态学杂志》 CAS CSCD 北大核心 2024年第2期487-493,共7页
以湖南省祁阳县的国家红壤肥力与肥料效益长期试验地为平台,采用单施氮肥(N)、氮钾配施(NK)、氮磷配施(NP)和氮磷钾配施(NPK)4种不同组合的无机肥料添加方式,结合实时定量PCR和高通量测序技术,探究长期施用化肥对土壤磷形态、磷酸酶活性... 以湖南省祁阳县的国家红壤肥力与肥料效益长期试验地为平台,采用单施氮肥(N)、氮钾配施(NK)、氮磷配施(NP)和氮磷钾配施(NPK)4种不同组合的无机肥料添加方式,结合实时定量PCR和高通量测序技术,探究长期施用化肥对土壤磷形态、磷酸酶活性及phoD基因丰度和多样性的影响。结果表明:与单施氮肥处理相比,氮与磷、钾配施可以缓解土壤酸化趋势,氮磷配施能够显著增加土壤中各形态磷含量。与单施氮肥处理相比,氮磷、氮钾处理显著提高了土壤碱性磷酸酶活性、土壤总细菌丰度及phoD基因丰度;氮磷钾配施显著提高了土壤碱性磷酸酶活性和土壤总细菌丰度,但显著降低土壤phoD基因丰度。磷酸酶编码基因phoD基因群落的α-与β-多样性在处理间不存在显著差异,且所有处理中来源于假单胞菌属与紫色杆菌属的phoD基因分别超过总序列的45%。此外,链霉菌属、马赛菌属以及短波单胞菌属与土壤磷素存在显著的正相关关系。以上结果表明:在不同养分输入条件下,调节功能菌群丰度及磷酸酶活性的关键因素不同。氮磷钾的合理配施能够增加土壤磷含量和磷酸酶活性,改变phoD基因丰度和群落结构,提高土壤的供磷能力,但长期施用化肥会使土壤微生物物种趋于单一化。 展开更多
关键词 化肥 高通量测序 磷酸酶 phoD基因丰度和多样性
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环境因子对污水微生物丰度及多样性的影响 被引量:1
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作者 尤俊豪 宗永臣 +2 位作者 王俊 傅椿惠 唐世龙 《环保科技》 2022年第1期51-58,共8页
污水中污染物的去除与微生物的丰度、多样性有密切的相关性。了解活性污泥中微生物丰度与多样性受环境因子的影响,有助于对污水处理厂的实际操作提供参考。本文通过对已发表文献的数据收集,从纬度、海拔、温度、进水化学需氧量(COD)、氨... 污水中污染物的去除与微生物的丰度、多样性有密切的相关性。了解活性污泥中微生物丰度与多样性受环境因子的影响,有助于对污水处理厂的实际操作提供参考。本文通过对已发表文献的数据收集,从纬度、海拔、温度、进水化学需氧量(COD)、氨氮(NH_(3)-N)、总氮(TN)、总磷(TP)共7个影响因素,分析其对代表微生物丰度Chao指数和多样性Shannon指数的影响变化。揭示微生物丰度与多样性受环境因子的响应,同时展望对未来污水微生物的研究趋势,以期能够从微观角度为污水处理厂的调控提供理论参考。 展开更多
关键词 污水微生物 微生物丰度与多样性 环境因子
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Evaluation of fall armyworm resistance in maize germplasm lines using visual leaf injury rating and predator survey
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作者 Xinzhi Ni Wenwei Xu +1 位作者 Michael H. Blanco W. Paul Williams 《Insect Science》 SCIE CAS CSCD 2014年第5期541-555,共15页
After examining ear-colonizing pest resistance, 20 maize lines from the USDA- ARS Germplasm Enhancement of Maize (GEM) Program were evaluated for whorl-feeding fall armyworm (FAW) (Spodopterafrugiperda) resistan... After examining ear-colonizing pest resistance, 20 maize lines from the USDA- ARS Germplasm Enhancement of Maize (GEM) Program were evaluated for whorl-feeding fall armyworm (FAW) (Spodopterafrugiperda) resistance using 4 maize inbred lines as the resistant and susceptible controls. Both FAW injury ratings at 7- and 14-d after infestation, and predator abundance and diversity at whorl stage (V6-V8) were recorded in 2009 and 2010. The survey of the diversity and abundance of predators in each experimental plot were conducted 7 d after the FAW infestation. Of the 20 germplasm lines examined, 3 of them (i.e., entries 9, 15, and 19 that were derived from tropical maize germplasm lines were originated from Uruguay, Cuba, and Thailand, respectively) were identified as the best FAW-resistant germplasm lines using the leaf injury ratings and predator survey data. In addition, the abundance and diversity of the predators were greater in 2010 than in 2009, which might have caused the low level of the FAW injury ratings on all lines examined in 2010. The 2-year data showed that the FAW injury ratings were negatively correlated to the predator abundance and diversity, which is also influence by genotype × environment interactions. The findings suggested that tropical germplasm is an important source of native resistance to the FAW and the corn earworm. At the same time, the maize genotype x environment interaction (e.g., predator attractiveness, and varying weather conditions) should be included in the multiple-year evaluations of insect and disease resistance of maize germplasm lines under field conditions. 展开更多
关键词 field screening genotype × environment interactions multiple insect resistance predator abundance and diversity whorl-feeding
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土壤多样性理论方法的新近发展与应用 被引量:29
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作者 张学雷 陈杰 +1 位作者 檀满枝 龚子同 《地球科学进展》 CAS CSCD 2003年第3期374-379,共6页
Abundance distribution models and diversity-area relationships are tools of biological diversity analysis that have been used by ecologists for decades. In a case study by Ibez et al., these techniques are considered ... Abundance distribution models and diversity-area relationships are tools of biological diversity analysis that have been used by ecologists for decades. In a case study by Ibez et al., these techniques are considered in a mor e general setting, and have been applied to explore notions such as pedodiversity (as an example of geodiversity in a broad sense, including also geomorphic diversity), in order to detect the differences and similarities between both natural resources, biological and non-biological.The discussion has mainly been conducted through the study of the Aegean Islands by the Spanish research team. Standard statistical techniques have been applied to analyze how the pedotaxa-abundance distribution conforms to the abundance distribution models and how pedorichness-area data fit to the diversity-area models.No statistically significant difference has been observed between the abundance distribution models and the diversity-area relationships followed by biodiversity and pedodiversity data in similar situations. Thus, the studied results may suggest that some assumptions underlying biodiversity analysis ought to be carefully re-examined.Since results in ecological literature are usually interpreted in biological terms, the analysis by Ibáez et al. may be relevant to offer some suggestions to the following questions: What are the reasons for the similarities obtained between biotic and soil resources? Should the ecological theory modify some of its constructs once the said similarities have been proven? and what are its implications for environmental management and assessment? 展开更多
关键词 土壤多样性 多度分布模型、丰富度与面积曲线 土壤与生物类型单元的关系
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