为了评价谷蛋白溶涨指数(SIG,Swelling index of glutenin)在小麦品质育种中的应用价值,检测了不同材料的面粉、配粉以及全麦粉的Zeleny沉淀值、SDS沉淀值和SIG,并分析了SIG与Zeleny沉淀值和SDS沉淀值的相关性。结果表明,基因型和种植...为了评价谷蛋白溶涨指数(SIG,Swelling index of glutenin)在小麦品质育种中的应用价值,检测了不同材料的面粉、配粉以及全麦粉的Zeleny沉淀值、SDS沉淀值和SIG,并分析了SIG与Zeleny沉淀值和SDS沉淀值的相关性。结果表明,基因型和种植环境各不相同的160份面粉样品的SIG与Zeleny沉淀值和SDS沉淀值之间均呈显著正相关,配粉的SIG能较好地反映出样品中面筋含量和质量的差异,说明SIG是一个比较可靠的品质评价指标;纯合品种面粉的SIG、Zeleny沉淀值、全麦粉的SIG、SDS沉淀值之间的相关性均达1%显著水平,其中全麦粉SIG与Zeleny沉淀值(面粉)相关更为密切,说明全麦粉的SIG与Zeleny沉淀值一样稳定有效,可以弥补Zeleny沉淀法不能用于全麦粉测试的缺憾;在广泛分离的育种群体中验证,SIG方法可成功应用于分离世代单株全麦粉的品质筛选。展开更多
In order to understand the genetic basis for Zeleny sedimentation value （ZSV） of wheat, a doubled haploid （DH） population Huapei 3 × Yumai 57 （Yumai 57 is superior to Huapei 3 for ZSV）, and a linkage map co...In order to understand the genetic basis for Zeleny sedimentation value （ZSV） of wheat, a doubled haploid （DH） population Huapei 3 × Yumai 57 （Yumai 57 is superior to Huapei 3 for ZSV）, and a linkage map consisting of 323 marker loci were used to search QTLs for ZSV. This program was based on mixed linear models and allowed simultaneous mapping of additive effect QTLs, epistatic QTLs, and QTL x environment interactions （QEs）. The DH population and the parents were evaluated for ZSV in three field trials. Mapping analysis produced a total of 8 QTLs and 2 QEs for ZSV with a single QTL explaining 0.64-14.39% of phenotypic variations. Four additive QTLs, 4 pairs of epistatic QTLs, and two QEs collectively explained 46.11% of the phenotypic variation （PVE）. This study provided a precise location of ZSV gene within the Xwmc 93 and GluD1 interval, which was designated as Qzsv-1D. The information obtained in this study should be useful for manipulating the QTLs for ZSV by marker assisted selection （MAS） in wheat breeding programs.展开更多
文摘为了评价谷蛋白溶涨指数(SIG,Swelling index of glutenin)在小麦品质育种中的应用价值,检测了不同材料的面粉、配粉以及全麦粉的Zeleny沉淀值、SDS沉淀值和SIG,并分析了SIG与Zeleny沉淀值和SDS沉淀值的相关性。结果表明,基因型和种植环境各不相同的160份面粉样品的SIG与Zeleny沉淀值和SDS沉淀值之间均呈显著正相关,配粉的SIG能较好地反映出样品中面筋含量和质量的差异,说明SIG是一个比较可靠的品质评价指标;纯合品种面粉的SIG、Zeleny沉淀值、全麦粉的SIG、SDS沉淀值之间的相关性均达1%显著水平,其中全麦粉SIG与Zeleny沉淀值(面粉)相关更为密切,说明全麦粉的SIG与Zeleny沉淀值一样稳定有效,可以弥补Zeleny沉淀法不能用于全麦粉测试的缺憾;在广泛分离的育种群体中验证,SIG方法可成功应用于分离世代单株全麦粉的品质筛选。
基金supported by the National Basic Re-search Program of China (2009CB118301)the National High-Tech Research and Development (863) Programof China (2006AA100101 and 2006AA10Z1E9)the Doctor Foundation of Shandong Agricultural University, China (23023)
文摘In order to understand the genetic basis for Zeleny sedimentation value （ZSV） of wheat, a doubled haploid （DH） population Huapei 3 × Yumai 57 （Yumai 57 is superior to Huapei 3 for ZSV）, and a linkage map consisting of 323 marker loci were used to search QTLs for ZSV. This program was based on mixed linear models and allowed simultaneous mapping of additive effect QTLs, epistatic QTLs, and QTL x environment interactions （QEs）. The DH population and the parents were evaluated for ZSV in three field trials. Mapping analysis produced a total of 8 QTLs and 2 QEs for ZSV with a single QTL explaining 0.64-14.39% of phenotypic variations. Four additive QTLs, 4 pairs of epistatic QTLs, and two QEs collectively explained 46.11% of the phenotypic variation （PVE）. This study provided a precise location of ZSV gene within the Xwmc 93 and GluD1 interval, which was designated as Qzsv-1D. The information obtained in this study should be useful for manipulating the QTLs for ZSV by marker assisted selection （MAS） in wheat breeding programs.
