探究元胡止痛滴丸治疗原发性痛经的多成分、多靶点、多途径作用机制,建立其"化合物-靶点-通路-疾病"的网络药理研究模式。针对元胡止痛滴丸中28个入血成分(22个原型化合物及6个代谢产物),利用Pharm Mapper服务器建立化合物-...探究元胡止痛滴丸治疗原发性痛经的多成分、多靶点、多途径作用机制,建立其"化合物-靶点-通路-疾病"的网络药理研究模式。针对元胡止痛滴丸中28个入血成分(22个原型化合物及6个代谢产物),利用Pharm Mapper服务器建立化合物-靶蛋白网络,通过Kyoto Encyclopedia of Genes and Genomes(KEGG)数据库对获取的靶点进行相关通路分析,最后利用Cytoscape软件构建元胡止痛滴丸的"化合物-靶点-通路-疾病"网络图。预测实验表明,28个入血成分的作用靶点约为109个,共涉及通路111条,其中52个靶点蛋白和31条通路与原发性痛经相关。元胡止痛滴丸可能通过作用于和激素调节、中枢镇痛、解痉、炎症及免疫相关的蛋白靶点及通路,起到治疗原发性痛经的作用。展开更多
Objective To establish an effective approach for rapid and comprehensive analysis on the absorbed and metabolic components in rats after ig administration of Yuanhu Zhitong Dropping Pill（YHZT）. Methods Based on the ...Objective To establish an effective approach for rapid and comprehensive analysis on the absorbed and metabolic components in rats after ig administration of Yuanhu Zhitong Dropping Pill（YHZT）. Methods Based on the combination of UPLC-Q-TOF/MS and multivariate statistical analysis, the absorbed prototype constituents and their metabolites in rat plasma were rapidly analyzed and identified, and the components absorbed into brain were further identified by comparing the extracted ion chromatograms（EICs） of control and brain tissue samples of dosed rats. Results A total of 38 YHZT-related xenobiotic compounds were detected and identified as the potential bioactive constituents in rat plasma, including 24 absorbed prototype constituents and 14 metabolites. In particular, of all prototype constituents, 14 were also detected in rat brain tissue, indicating that they could penetrate the blood-brain barrier and enter into brain. Conclusion An effective method is established and applied to analyze the potential bioactive constituents in YHZT, which provides a pathway to further investigate the pharmacological pattern and mechanism of YHZT.展开更多
文摘探究元胡止痛滴丸治疗原发性痛经的多成分、多靶点、多途径作用机制,建立其"化合物-靶点-通路-疾病"的网络药理研究模式。针对元胡止痛滴丸中28个入血成分(22个原型化合物及6个代谢产物),利用Pharm Mapper服务器建立化合物-靶蛋白网络,通过Kyoto Encyclopedia of Genes and Genomes(KEGG)数据库对获取的靶点进行相关通路分析,最后利用Cytoscape软件构建元胡止痛滴丸的"化合物-靶点-通路-疾病"网络图。预测实验表明,28个入血成分的作用靶点约为109个,共涉及通路111条,其中52个靶点蛋白和31条通路与原发性痛经相关。元胡止痛滴丸可能通过作用于和激素调节、中枢镇痛、解痉、炎症及免疫相关的蛋白靶点及通路,起到治疗原发性痛经的作用。
基金National Natural Science Foundation of China(No.81430096)
文摘Objective To establish an effective approach for rapid and comprehensive analysis on the absorbed and metabolic components in rats after ig administration of Yuanhu Zhitong Dropping Pill（YHZT）. Methods Based on the combination of UPLC-Q-TOF/MS and multivariate statistical analysis, the absorbed prototype constituents and their metabolites in rat plasma were rapidly analyzed and identified, and the components absorbed into brain were further identified by comparing the extracted ion chromatograms（EICs） of control and brain tissue samples of dosed rats. Results A total of 38 YHZT-related xenobiotic compounds were detected and identified as the potential bioactive constituents in rat plasma, including 24 absorbed prototype constituents and 14 metabolites. In particular, of all prototype constituents, 14 were also detected in rat brain tissue, indicating that they could penetrate the blood-brain barrier and enter into brain. Conclusion An effective method is established and applied to analyze the potential bioactive constituents in YHZT, which provides a pathway to further investigate the pharmacological pattern and mechanism of YHZT.
