本试验通过PCR-RFLP技术对大白猪、长白猪和杜洛克猪的视黄醇结合蛋白4(retinol binding protein 4,RBP4)基因第4外显子MspⅠ酶切位点进行多态性分析,并对各基因型母猪的第2、3胎次产仔数、产活仔数、断奶仔猪数、初生窝重、21日龄的窝...本试验通过PCR-RFLP技术对大白猪、长白猪和杜洛克猪的视黄醇结合蛋白4(retinol binding protein 4,RBP4)基因第4外显子MspⅠ酶切位点进行多态性分析,并对各基因型母猪的第2、3胎次产仔数、产活仔数、断奶仔猪数、初生窝重、21日龄的窝重及断奶成活率进行测定,进而分析RBP4基因多态性与繁殖性能的关系。结果表明,RBP4基因在3个品种中均存在多态位点;χ2适合性检验表明大白猪在该位点偏离Hardy-Weinberg平衡状态(P<0.05),而长白猪和杜洛克猪在该位点处于Hardy-Weinberg平衡状态(P>0.05)。其中,大白猪中优势基因型为BB基因型,而长白猪和杜洛克猪中优势基因型为AA基因型。在繁殖性能上,长白猪BB基因型群体第2胎总产仔数、产活仔数、断奶仔猪数、初生窝重、21日龄断奶窝重分别高于AA基因型0.97头、1.28头、0.92头、1.74kg和4.42kg,高于AB基因型0.92头、1.02头、0.98头、1.43kg和4.98kg;第3胎次中总体上BB基因型也为有利基因型。展开更多
本研究提取251头大白母猪毛囊基因组,以PCR-RFLP法对繁殖性状候选基因FSH-β(促卵泡素β亚基)、ESR(雌激素受体)和BF(备解素因子)进行基因分型和遗传效应分析。结果表明:该猪群3个基因的优势等位基因分别为B、B和A;初产母猪FSH-β基因...本研究提取251头大白母猪毛囊基因组,以PCR-RFLP法对繁殖性状候选基因FSH-β(促卵泡素β亚基)、ESR(雌激素受体)和BF(备解素因子)进行基因分型和遗传效应分析。结果表明:该猪群3个基因的优势等位基因分别为B、B和A;初产母猪FSH-β基因的AB基因型健仔数和初生窝重分别比AA型多1.21头和1.60 kg(P<0.05),经产母猪AB和BB型初生窝重比AA型分别多1.42 kg和1.24 kg(P<0.05);BF基因的AA型初产母猪出生窝重高出AB型母猪1.54 kg(P<0.05);FSH-β、ESR、BF的优势合并基因型为AB AA AA,含此基因型母猪的全仔数、健仔数、初生窝重比最低的AA AB AA型母猪分别高1.44、1.45、2.21 kg(P<0.01);个体育种值估计结果表明,AB AA AA BB AA AA等合并基因型母猪的全仔数及健仔数排名该猪群前10名。本试验结果为大白猪繁殖性状的分子标记辅助选择提供了参考。展开更多
This study was conducted to compare physiological characteristics between Meishan and Yorkshire piglets in their early lives. Six healthy purebred Meishan sows and Yorkshire sows with close farrowing dates were used i...This study was conducted to compare physiological characteristics between Meishan and Yorkshire piglets in their early lives. Six healthy purebred Meishan sows and Yorkshire sows with close farrowing dates were used in this research. The piglets sucked their respective sow’s milk for 14 days, then they were slaughtered to collect samples of blood, pancreas, contents of stomach, jejunum, cecum, colon as well as feces for analysis of blood biochemical parameters, digestive enzymes, and volatile fatty acid(VFA). The results showed that Yorkshire piglets had higher concentrations of high-density lipoprotein cholesterol(HDL-C) and total cholesterol(TC)(P < 0.05). Gastric lipase activity was higher in Meishan piglets but Yorkshire piglets had higher lactase activity(P < 0.05). The total VFA together with acetate and propionate in cecum and colon were higher in Meishan piglets than in Yorkshire piglets(P < 0.05),but acetate in jejunum and ratio of acetate to propionate in colon were lower in Meishan piglets than in Yorkshire piglets(P < 0.05). In conclusion, in early suckling period, significant differences exist in host metabolism and intestinal microbial metabolism between Meishan and Yorkshire piglets.展开更多
This study aims to compare the meat quality of Shaziling and Yorkshire pigs and to find the potential indicator in serum for superior meat quality.Six Shaziling and Yorkshire pigs at 30,60,90,150,210,and 300 d of age ...This study aims to compare the meat quality of Shaziling and Yorkshire pigs and to find the potential indicator in serum for superior meat quality.Six Shaziling and Yorkshire pigs at 30,60,90,150,210,and 300 d of age were selected to examine carcass traits,meat quality,and serum metabolome.The results showed that the body weight,carcass length,and loin eye area of Shaziling pigs at 150,210,and 300 d of age were significantly lower than those of Yorkshire pigs(P<0.05).Shaziling pigs at 150 and 300 d of age had significantly lower backfat thickness than Yorkshire pigs(P<0.05).Compared with Yorkshire pigs,Shaziling pigs at all 6 ages had a lower lean percentage and a higher fat percentage(P<0.05).At 60,90,and 150 d of age,the post-mortem pH-decline,b*value(yellowness),and drip loss of Shaziling pigs were significantly lower than those of Yorkshire pigs(P<0.05).Moreover,at 150 d of age,Shaziling pigs had significantly higher a^(*)value(redness)and intramuscular fat(IMF)content than Yorkshire pigs(P<0.05).Correlation analysis between the top 40 metabolites and phenotypes indicated that L-carnitine had positive correlations with fat percentage,pH24h,and IMF content,but had negative correlations with lean percentage,L^(*)value(lightness),and b^(*)value(P<0.05).Serum L-carnitine content,fat percentage,pH24h,and IMF content all decreased first and then increased as the pigs grew,which verified the positive correlations between L-carnitine and these phenotypes.In conclusion,Shaziling pigs have a slower growth rate but a better meat quality than Yorkshire pigs.The meat quality of Shaziling pigs is the best from 150 to 210 d of age.This study suggests that a higher serum L-carnitine content is a promising indicator for better meat quality.展开更多
[Objective] This study was to investigate the genetic parameters on reproduction performances of China Yorkshire line SII,. [Method] Using China Yorkshire line SII, as experimental animal, its genetic parameters on re...[Objective] This study was to investigate the genetic parameters on reproduction performances of China Yorkshire line SII,. [Method] Using China Yorkshire line SII, as experimental animal, its genetic parameters on reproduction performance, such as total number of born （TNB）, the alive number of born （ANB）, the litter weight of born （LWB） and the litter weight at age of 21 days （/W21 ） , were estimated by using the DFREML method of multi-traits animal model. [Result] The heritability estimates of TNB, ANB, LWB and LW21 were 0.11,0.19, 0.14 and 0.05, respectively; and the genetic correlations between the traits as TNB/ANB, TNB/LWB, TNB/LW21, ANB/LWB, ANB/LW21, LWB/LW21 were 0.91, 0.76, 0.65, 0.85, 0.59 and 0.38, respectively. [ Conclusion] Main genetic parameters of reproduction traits in China Yorkshire line all, have all reached or even exceeded the requirements of breeding.展开更多
文摘本试验通过PCR-RFLP技术对大白猪、长白猪和杜洛克猪的视黄醇结合蛋白4(retinol binding protein 4,RBP4)基因第4外显子MspⅠ酶切位点进行多态性分析,并对各基因型母猪的第2、3胎次产仔数、产活仔数、断奶仔猪数、初生窝重、21日龄的窝重及断奶成活率进行测定,进而分析RBP4基因多态性与繁殖性能的关系。结果表明,RBP4基因在3个品种中均存在多态位点;χ2适合性检验表明大白猪在该位点偏离Hardy-Weinberg平衡状态(P<0.05),而长白猪和杜洛克猪在该位点处于Hardy-Weinberg平衡状态(P>0.05)。其中,大白猪中优势基因型为BB基因型,而长白猪和杜洛克猪中优势基因型为AA基因型。在繁殖性能上,长白猪BB基因型群体第2胎总产仔数、产活仔数、断奶仔猪数、初生窝重、21日龄断奶窝重分别高于AA基因型0.97头、1.28头、0.92头、1.74kg和4.42kg,高于AB基因型0.92头、1.02头、0.98头、1.43kg和4.98kg;第3胎次中总体上BB基因型也为有利基因型。
文摘本研究提取251头大白母猪毛囊基因组,以PCR-RFLP法对繁殖性状候选基因FSH-β(促卵泡素β亚基)、ESR(雌激素受体)和BF(备解素因子)进行基因分型和遗传效应分析。结果表明:该猪群3个基因的优势等位基因分别为B、B和A;初产母猪FSH-β基因的AB基因型健仔数和初生窝重分别比AA型多1.21头和1.60 kg(P<0.05),经产母猪AB和BB型初生窝重比AA型分别多1.42 kg和1.24 kg(P<0.05);BF基因的AA型初产母猪出生窝重高出AB型母猪1.54 kg(P<0.05);FSH-β、ESR、BF的优势合并基因型为AB AA AA,含此基因型母猪的全仔数、健仔数、初生窝重比最低的AA AB AA型母猪分别高1.44、1.45、2.21 kg(P<0.01);个体育种值估计结果表明,AB AA AA BB AA AA等合并基因型母猪的全仔数及健仔数排名该猪群前10名。本试验结果为大白猪繁殖性状的分子标记辅助选择提供了参考。
基金supported by National Key Basic Research Program of China(973 Program)(2013CB127300)the EU 7th Framework Program(FP7-KBBE-2008-2B)+2 种基金Natural Science Foundation of China(31430082)Jiangsu Province Natural Science Foundation(BK20130058)Research Fund for the Doctoral Program of Higher Education of China(20130097130005)
文摘This study was conducted to compare physiological characteristics between Meishan and Yorkshire piglets in their early lives. Six healthy purebred Meishan sows and Yorkshire sows with close farrowing dates were used in this research. The piglets sucked their respective sow’s milk for 14 days, then they were slaughtered to collect samples of blood, pancreas, contents of stomach, jejunum, cecum, colon as well as feces for analysis of blood biochemical parameters, digestive enzymes, and volatile fatty acid(VFA). The results showed that Yorkshire piglets had higher concentrations of high-density lipoprotein cholesterol(HDL-C) and total cholesterol(TC)(P < 0.05). Gastric lipase activity was higher in Meishan piglets but Yorkshire piglets had higher lactase activity(P < 0.05). The total VFA together with acetate and propionate in cecum and colon were higher in Meishan piglets than in Yorkshire piglets(P < 0.05),but acetate in jejunum and ratio of acetate to propionate in colon were lower in Meishan piglets than in Yorkshire piglets(P < 0.05). In conclusion, in early suckling period, significant differences exist in host metabolism and intestinal microbial metabolism between Meishan and Yorkshire piglets.
基金This study was jointly supported by the National Natural Science Foundation of China(U19A2037,31802077)the Changsha Natural Science Funds for Distinguished Young Scholar(kq2009020)+6 种基金the Natural Science Foundation of Guangxi Province(2020JJA130102,2018JJB130239)Young Elite Scientists Sponsorship Program by CAST(2020QNRC001)Special funds for the construction of innovative provinces in Hunan Project(2019NK2193,2019RS3022)China Agriculture Research System of MOF and MARA(CARS-35)the‘Strategic Priority Research Program’of the Chinese Academy of Sciences(XDA24030204)Open Fund of Key Laboratory of Agroecological Processes in Subtropical Region,Chinese Academy of Sciences(ISA2020203)Taishan industry leading talent project special funds.
文摘This study aims to compare the meat quality of Shaziling and Yorkshire pigs and to find the potential indicator in serum for superior meat quality.Six Shaziling and Yorkshire pigs at 30,60,90,150,210,and 300 d of age were selected to examine carcass traits,meat quality,and serum metabolome.The results showed that the body weight,carcass length,and loin eye area of Shaziling pigs at 150,210,and 300 d of age were significantly lower than those of Yorkshire pigs(P<0.05).Shaziling pigs at 150 and 300 d of age had significantly lower backfat thickness than Yorkshire pigs(P<0.05).Compared with Yorkshire pigs,Shaziling pigs at all 6 ages had a lower lean percentage and a higher fat percentage(P<0.05).At 60,90,and 150 d of age,the post-mortem pH-decline,b*value(yellowness),and drip loss of Shaziling pigs were significantly lower than those of Yorkshire pigs(P<0.05).Moreover,at 150 d of age,Shaziling pigs had significantly higher a^(*)value(redness)and intramuscular fat(IMF)content than Yorkshire pigs(P<0.05).Correlation analysis between the top 40 metabolites and phenotypes indicated that L-carnitine had positive correlations with fat percentage,pH24h,and IMF content,but had negative correlations with lean percentage,L^(*)value(lightness),and b^(*)value(P<0.05).Serum L-carnitine content,fat percentage,pH24h,and IMF content all decreased first and then increased as the pigs grew,which verified the positive correlations between L-carnitine and these phenotypes.In conclusion,Shaziling pigs have a slower growth rate but a better meat quality than Yorkshire pigs.The meat quality of Shaziling pigs is the best from 150 to 210 d of age.This study suggests that a higher serum L-carnitine content is a promising indicator for better meat quality.
基金supported by National Key Technology R&D Program(2006BAD01A08-02)Program from Hubei Innovation Center of Agricultural Science and Technology(2007-620-004-03)Special Fund for Constructing National Technology System for Pig Industry(NYCYTX-009)
文摘[Objective] This study was to investigate the genetic parameters on reproduction performances of China Yorkshire line SII,. [Method] Using China Yorkshire line SII, as experimental animal, its genetic parameters on reproduction performance, such as total number of born （TNB）, the alive number of born （ANB）, the litter weight of born （LWB） and the litter weight at age of 21 days （/W21 ） , were estimated by using the DFREML method of multi-traits animal model. [Result] The heritability estimates of TNB, ANB, LWB and LW21 were 0.11,0.19, 0.14 and 0.05, respectively; and the genetic correlations between the traits as TNB/ANB, TNB/LWB, TNB/LW21, ANB/LWB, ANB/LW21, LWB/LW21 were 0.91, 0.76, 0.65, 0.85, 0.59 and 0.38, respectively. [ Conclusion] Main genetic parameters of reproduction traits in China Yorkshire line all, have all reached or even exceeded the requirements of breeding.
