A total of 64 patients with acute lacunar infarction were enrolled within 24 hours of onset. The patients received conventional therapy （antiplatelet drugs and hypolipidemic drugs） alone or conventional therapy plus...A total of 64 patients with acute lacunar infarction were enrolled within 24 hours of onset. The patients received conventional therapy （antiplatelet drugs and hypolipidemic drugs） alone or conventional therapy plus 450 mg Xueshuantong once a day. The main ingredient of the Xueshuantong lyophilized powder used for injection was Panax notoginseng saponins. Assessments were made at admission and at discharge using the National Institutes of Health Stroke Scale, the Activity of Daily Living and the Mini-Mental State Examination. Additionally, the relative cerebral blood flow, relative cerebral blood volume and relative mean transit time in the region of interest were calculated within 24 hours after the onset of lacunar infarction, using dynamic susceptibility contrast magnetic resonance perfusion imaging technology. Patients underwent a follow-up MRI scan after 4 weeks of treatment. There was an improvement in the Activity of Daily Living scores and a greater reduction in the scores on the National Institutes of Health Stroke Scale in the treatment group than in the control group. However, the Mini-Mental State Examination scores showed no significant differences after 4 weeks of treatment. Compared with the control group, the relative cerebral blood flow at discharge had increased and showed a greater improvement in the treatment group. Furthermore, there was a reduction in the relative mean transit time at discharge and the value was lower in the treatment group than in the control group. The experimental findings indicate that Xueshuantong treatment improves neurological deficits in elderly patients with lacunar infarction, and the mechanism may be related to increased cerebral perfusion.展开更多
目的:观察注射用血栓通(主要成分为三七总皂苷)对过氧化氢与肿瘤坏死因子(TNF-α)诱导大鼠血管内皮细胞凋亡的保护效应,并对其干预血管内皮细胞的凋亡通路和机制进行研究,为注射用血栓通更广泛的临床应用提供试验依据。方法:针对体外培...目的:观察注射用血栓通(主要成分为三七总皂苷)对过氧化氢与肿瘤坏死因子(TNF-α)诱导大鼠血管内皮细胞凋亡的保护效应,并对其干预血管内皮细胞的凋亡通路和机制进行研究,为注射用血栓通更广泛的临床应用提供试验依据。方法:针对体外培养的大鼠肺部血管内皮细胞,施加过氧化氢与TNF-α等凋亡诱导因子,建立血管内皮细胞凋亡诱导模型,继而采用50、100μg/mL等浓度的注射用血栓通进行预干预,并采用Annexin V Ca2+依赖性磷脂结合蛋白/PI碘化丙啶染色法检测血管内皮细胞凋亡率以及采用细胞线粒体膜电位变化反映细胞线粒体损伤率,进而检测凋亡相关通路调控基因Caspase-3、Bcl-2和Fas的表达情况。结果:注射用血栓通浓度为100μg/mL时,能使过氧化氢诱导的大鼠血管内皮细胞凋亡率与线粒体损伤率显著降低,但对TNF-α诱导的细胞凋亡无显著的抑制作用,进一步研究发现以100μg/mL的注射用血栓通可明显减低过氧化氢诱导的Caspase-3表达,而且还可明显提高Bcl-2的水平,但对Fas表达的影响不明显。结论:注射用血栓通(三七总皂苷)可通过下调Caspase-3表达与上调Bcl-2基因来抑制氧化应激诱导的血管内皮细胞凋亡。展开更多
基金supported by the Technological Foundation Project of Traditional Chinese Medicine Science of Zhejiang Province,No.2012ZA077
文摘A total of 64 patients with acute lacunar infarction were enrolled within 24 hours of onset. The patients received conventional therapy （antiplatelet drugs and hypolipidemic drugs） alone or conventional therapy plus 450 mg Xueshuantong once a day. The main ingredient of the Xueshuantong lyophilized powder used for injection was Panax notoginseng saponins. Assessments were made at admission and at discharge using the National Institutes of Health Stroke Scale, the Activity of Daily Living and the Mini-Mental State Examination. Additionally, the relative cerebral blood flow, relative cerebral blood volume and relative mean transit time in the region of interest were calculated within 24 hours after the onset of lacunar infarction, using dynamic susceptibility contrast magnetic resonance perfusion imaging technology. Patients underwent a follow-up MRI scan after 4 weeks of treatment. There was an improvement in the Activity of Daily Living scores and a greater reduction in the scores on the National Institutes of Health Stroke Scale in the treatment group than in the control group. However, the Mini-Mental State Examination scores showed no significant differences after 4 weeks of treatment. Compared with the control group, the relative cerebral blood flow at discharge had increased and showed a greater improvement in the treatment group. Furthermore, there was a reduction in the relative mean transit time at discharge and the value was lower in the treatment group than in the control group. The experimental findings indicate that Xueshuantong treatment improves neurological deficits in elderly patients with lacunar infarction, and the mechanism may be related to increased cerebral perfusion.
文摘目的:观察注射用血栓通(主要成分为三七总皂苷)对过氧化氢与肿瘤坏死因子(TNF-α)诱导大鼠血管内皮细胞凋亡的保护效应,并对其干预血管内皮细胞的凋亡通路和机制进行研究,为注射用血栓通更广泛的临床应用提供试验依据。方法:针对体外培养的大鼠肺部血管内皮细胞,施加过氧化氢与TNF-α等凋亡诱导因子,建立血管内皮细胞凋亡诱导模型,继而采用50、100μg/mL等浓度的注射用血栓通进行预干预,并采用Annexin V Ca2+依赖性磷脂结合蛋白/PI碘化丙啶染色法检测血管内皮细胞凋亡率以及采用细胞线粒体膜电位变化反映细胞线粒体损伤率,进而检测凋亡相关通路调控基因Caspase-3、Bcl-2和Fas的表达情况。结果:注射用血栓通浓度为100μg/mL时,能使过氧化氢诱导的大鼠血管内皮细胞凋亡率与线粒体损伤率显著降低,但对TNF-α诱导的细胞凋亡无显著的抑制作用,进一步研究发现以100μg/mL的注射用血栓通可明显减低过氧化氢诱导的Caspase-3表达,而且还可明显提高Bcl-2的水平,但对Fas表达的影响不明显。结论:注射用血栓通(三七总皂苷)可通过下调Caspase-3表达与上调Bcl-2基因来抑制氧化应激诱导的血管内皮细胞凋亡。
