Shenqi Wuweizi tablet is a Chinese medicine preparation mainly composed of Codonopsis pilosula, Astragalus membranaceus, Kadsura longipedunculata and Ziziphus jujube with many curative effects. The maximum tolerance d...Shenqi Wuweizi tablet is a Chinese medicine preparation mainly composed of Codonopsis pilosula, Astragalus membranaceus, Kadsura longipedunculata and Ziziphus jujube with many curative effects. The maximum tolerance dose of Shenqi Wuweizi tablet in mice was studied, and its acute toxicity in mice was ex- plored. The results showed that Shenqi Wuweizi tablet did not cause the death of mice at the maximum dosage and mice did not show toxic reaction, so it could be used in livestock production and clinical veterinary treatment. The study provided a theoretical basis for application of Shenqi Wuweizi tablet in livestock production and clinical veterinary medication safety.展开更多
文摘目的:本文在于探讨五味子通过促进内源性干细胞增殖治疗肝硬化的机制.方法:通过CCl_4复制大鼠肝硬化模型成功后,将大鼠随机分成实验组(小剂量组A1、中剂量组A2和大剂量组A3)、空白组C及模型组M,小剂量组给予五味子1.0 mg/200 g,中剂量组给予1.5 mg/200 g,大剂量给予2.0 mg/200 g.观察五味子对肝硬化大鼠肝脏组织病理学、肝功[谷草转氨酶(aspartate transaminase,AST)、谷丙转氨酶(alanine transaminase,ALT)和白蛋白(albnmin,ALB)]、肝纤维化的影响以及通过免疫荧光法和免疫组织化学法观察五味子对Brdu标记的内源性干细胞的增殖情况及CK-18、ALB及甲胎蛋白(α-fetoprotein,AFP)三种蛋白的表达情况.结果:通过病理切片HE染色及Masson染色,治疗组的肝硬化大鼠肝细胞变性坏死及肝纤维化程度有一定的改善,各治疗组的肝纤维化评分各异(P<0.05),与模型组相比,中剂量组的肝纤维化评分改善最明显(P<0.01),在免疫组织化学中,发现各治疗组ALB、C K-18及A F P的蛋白表达不同(P<0.05),用B r d u标记肝脏处于分裂间期的干细胞,通过"区域共存"观察到各治疗组干细胞的增殖和分裂,并用免疫荧光法半定量统计发现,各治疗组中ALB、CK-18及AFP的蛋白表达不同(P<0.01),中剂量组表达最显著(P<0.01),在从眼球采血评估肝硬化大鼠肝功能中,各治疗组的肝功能均有一定程度的改善(P<0.05),与对照组相比,中剂量组的AST、ALT明显下降,ALB明显改善(P<0.01).结论:五味子对损伤的肝脏细胞有保护作用,并能延缓肝硬化的进展,机制有可能与五味子通过促进内源性干细胞增殖防治肝硬化有关.
基金Supported by"Veterinary Biological Technology"Innovation Team Project of Colleges and Universities in Tianjin(TD12-5019)Teaching Reform Project of Experimental Teaching Demonstration Center at Tianjin Agricultural University(2015SY102)+2 种基金Innovative Team Training Program Fund of Colleges and Universities in Tianjin(TNTD2015015)Science and Technology Development Fund of Tianjin Agricultural University(2013N09)Innovative Entre-preneurial Training Program of College Students(201510061166)
文摘Shenqi Wuweizi tablet is a Chinese medicine preparation mainly composed of Codonopsis pilosula, Astragalus membranaceus, Kadsura longipedunculata and Ziziphus jujube with many curative effects. The maximum tolerance dose of Shenqi Wuweizi tablet in mice was studied, and its acute toxicity in mice was ex- plored. The results showed that Shenqi Wuweizi tablet did not cause the death of mice at the maximum dosage and mice did not show toxic reaction, so it could be used in livestock production and clinical veterinary treatment. The study provided a theoretical basis for application of Shenqi Wuweizi tablet in livestock production and clinical veterinary medication safety.
