目的:探讨Wnt蛋白生成抑制剂2(the inhibitor of Wnt production 2,IWP2)对非小细胞肺癌细胞迁移与侵袭能力的影响。方法:体外培养非小细胞肺癌H1299和95C细胞,分为对照组(无血清培养基)及实验组(应用10μmol/L IWP2处理24或48 h)。采...目的:探讨Wnt蛋白生成抑制剂2(the inhibitor of Wnt production 2,IWP2)对非小细胞肺癌细胞迁移与侵袭能力的影响。方法:体外培养非小细胞肺癌H1299和95C细胞,分为对照组(无血清培养基)及实验组(应用10μmol/L IWP2处理24或48 h)。采用划痕实验和未铺Matrigel胶的Transwell迁移实验检测IWP2对细胞迁移能力的影响;铺有Matrigel胶的Transwell侵袭实验检测IWP2对细胞侵袭能力的影响;Western blot实验检测IWP2对非小细胞肺癌细胞中β-catenin及上皮间充质转化(EMT)相关蛋白ZEB1、Snail表达的影响。结果:划痕实验结果显示,经10μmol/L IWP2处理24 h后,与对照组比较,实验组H1299和95C细胞的划痕愈合面积均明显降低(P<0.01);在Transwell迁移实验中,实验组过膜细胞数明显降低(P<0.01);Transwell侵袭实验结果显示,实验组细胞表现出更低的侵袭能力(P<0.01)。Western blot结果表明,H1299和95C细胞在经IWP2作用后,与对照组比较,β-catenin的表达水平分别降低41.3%和36.1%(P均<0.01);IWP2也抑制了EMT相关蛋白ZEB1、Snail的表达,与对照组比较,实验组H1299和95C细胞中,ZEB1的表达水平分别降低51.8%和40.9%,Snail表达水平分别降低43.2%和30.7%,差异均具有统计学意义(P均<0.01)。结论:IWP2抑制β-catenin的表达并抑制非小细胞肺癌细胞发生EMT,从而降低细胞的迁移与侵袭能力。展开更多
AIM To investigate the potential role of micro RNA-30 a(mi R-30 a) in esophageal squamous cell carcinoma(ESCC).METHODS Expression of mi R-30 a-3 p/5 p was analyzed using microarray data and fresh ESCC tissue samples. ...AIM To investigate the potential role of micro RNA-30 a(mi R-30 a) in esophageal squamous cell carcinoma(ESCC).METHODS Expression of mi R-30 a-3 p/5 p was analyzed using microarray data and fresh ESCC tissue samples. Both in vitro and in vivo assays were used to investigate the effects of mi R-30 a-3 p/5 p on ESCC cell proliferation. Furthermore,Kyoto Encyclopedia of Genes and Genomes analysis was performed to explore underlying mechanisms involved in ESCC,and then,assays were carried out to verify the potential molecular mechanism of mi R-30 a in ESCC.RESULTS Low expression of mi R-30 a-3 p/5 p was closely associated with advanced ESCC progression and poor prognosis of patients with ESCC. Knock-down of mi R-30 a-3 p/5 p promoted ESCC cell proliferation. Increased mi R-30 a-3 p/5 p expression inhibited the Wnt signaling pathway by targeting Wnt2 and Fzd2.CONCLUSION Down-regulation of mi R-30 a-3 p/5 p promotes ESCC cell proliferation by activating the Wnt signaling pathway through inhibition of Wnt2 and Fzd2.展开更多
基金Supported by the Youth Fund of the First Affiliated Hospital of Xinxiang Medical University(Type A-4)
文摘AIM To investigate the potential role of micro RNA-30 a(mi R-30 a) in esophageal squamous cell carcinoma(ESCC).METHODS Expression of mi R-30 a-3 p/5 p was analyzed using microarray data and fresh ESCC tissue samples. Both in vitro and in vivo assays were used to investigate the effects of mi R-30 a-3 p/5 p on ESCC cell proliferation. Furthermore,Kyoto Encyclopedia of Genes and Genomes analysis was performed to explore underlying mechanisms involved in ESCC,and then,assays were carried out to verify the potential molecular mechanism of mi R-30 a in ESCC.RESULTS Low expression of mi R-30 a-3 p/5 p was closely associated with advanced ESCC progression and poor prognosis of patients with ESCC. Knock-down of mi R-30 a-3 p/5 p promoted ESCC cell proliferation. Increased mi R-30 a-3 p/5 p expression inhibited the Wnt signaling pathway by targeting Wnt2 and Fzd2.CONCLUSION Down-regulation of mi R-30 a-3 p/5 p promotes ESCC cell proliferation by activating the Wnt signaling pathway through inhibition of Wnt2 and Fzd2.
基金Specialized Research Fund for the Doctoral Program of Higher Education(SRFDP)sponsored by the Ministry of Education of China (20070246184)Opened Fund sponsored by Institute of Brain Science,Fudan University(2007)Basic-clinic Cross Research Fund sponsored by Shanghai Medical College,Fudan University(2007-09)~~
