目的:观察针灸对急性带状疱疹患者血液c-FLIP表达的影响,初步探讨其治疗带状疱疹的机制。方法:选择急性带状疱疹患者25例,随机分为药物治疗组(P组,n=13)和针灸治疗组(A组,n=12),选择12例正常人作为对照。P组采用抗病毒、营养神经和镇痛...目的:观察针灸对急性带状疱疹患者血液c-FLIP表达的影响,初步探讨其治疗带状疱疹的机制。方法:选择急性带状疱疹患者25例,随机分为药物治疗组(P组,n=13)和针灸治疗组(A组,n=12),选择12例正常人作为对照。P组采用抗病毒、营养神经和镇痛药物治疗,A组在相同药物治疗基础上加针灸治疗。比较两组患者治疗前、治疗后7 d VAS评分和皮疹愈合时间;Western-blot法检测c-FLIP表达,比较正常对照组与急性带状疱疹患者及两组患者治疗前、后血液c-FLIP表达情况。结果:两组患者治疗后VAS评分较治疗前均明显减少(P<0.01),治疗后组间VAS评分比较差异无统计学意义,A组皮疹愈合时间短于P组(P<0.01);急性带状疱疹患者c-FLIP表达比正常人表达明显降低(P<0.01),A组治疗后较治疗前表达升高(P<0.01),P组治疗前、后表达无明显变化。结论:针灸可以调节急性带状疱疹患者血液c-FLIP表达,促进皮疹愈合。这可能是针灸治疗带状疱疹的细胞免疫机制之一。展开更多
用基因工程技术制备出的细粒棘球绦虫重组抗原 B( r Ag B)表达载体 ,经诱导表达、亲和层析纯化获得具生物活性的重组蛋白质 r Ag B,用 Western- Blot法检测病人血清。结果显示 r Ag B敏感性为 91.6% ( 4 4/48) ,特异性为 93 .8% ( 3 0 /...用基因工程技术制备出的细粒棘球绦虫重组抗原 B( r Ag B)表达载体 ,经诱导表达、亲和层析纯化获得具生物活性的重组蛋白质 r Ag B,用 Western- Blot法检测病人血清。结果显示 r Ag B敏感性为 91.6% ( 4 4/48) ,特异性为 93 .8% ( 3 0 /3 2 ) ,其中 10例泡球蚴 ( AE)病人及 10例肿瘤病人血清均无交叉反应。说明 r Ag B具有较高的敏感性及特异性 ,可用于包虫病的常规血清学诊断 ,r Ag B在宿主菌 JM10 9内稳定表达 ,因此可在实验室内大量制备用于血清学诊断的 r Ag B。展开更多
The high molecular weight glutenin subunit （HMW-GS） pair 1Bx13＋1By16 are recognized to positively correlate with bread-making quality; however, their molecular data remain unknown. In order to reveal the mechanism ...The high molecular weight glutenin subunit （HMW-GS） pair 1Bx13＋1By16 are recognized to positively correlate with bread-making quality; however, their molecular data remain unknown. In order to reveal the mechanism by which 1By16 and 1Bx13 creates high quality, their open reading frames （ORFs） were amplified from common wheat Atlas66 and Jimai 20 using primers that were designed based on published sequences of HMW glutenin genes. The ORF of 1By16 was 2220bp, deduced into 738 amino acid residues with seven cysteines including 59 hexapeptides and 22 nanopeptides motifs. The ORF of 1Bx13 was 2385bp, deduced into 795 amino acid residues with four cysteines including 68 hexapeptides, 25 nanopeptides and six tripepUdes motifs. We found that 1By16 was the largest y-type HMW glutenin gene described to date in common wheat. The 1By16 had 36 amino acid residues inserted in the central repetitive domain compared with 1By15. Expression in bacteria and western-blot tests confirmed that the sequence cloned was the ORF of HMW-GS 1By16, and that 1Bx13 was one of the largest 1Bx genes that have been described so far in common wheat, exhibiting a hexapeptide （PGQGQQ） insertion in the end of central repetitive domain compared with 1Bx7. A phylogenetic tree based on the deduced full-length amino acid sequence alignment of the published HMW-GS genes showed that the 1By16 was clustered with Glu-1B-2, and that the 1Bx13 was clustered with Glu-1B-1 alleles.展开更多
The proteins from endosperm, root, stem, leaf, leafstalk of Jatropha curcas L. and their calli were hybridized to the seed toxin protein (curcin) of Jatropha curcas L. by western blot. The result showed that the curci...The proteins from endosperm, root, stem, leaf, leafstalk of Jatropha curcas L. and their calli were hybridized to the seed toxin protein (curcin) of Jatropha curcas L. by western blot. The result showed that the curcin was specifically expressed in the endosperm and its calli, while it was not detected in root, stem, leaf, and leafstalk of Jatropha curcas L. and their calli . This study indicated that calli induced from endosperm can be used to produce curcin.展开更多
文摘目的:观察针灸对急性带状疱疹患者血液c-FLIP表达的影响,初步探讨其治疗带状疱疹的机制。方法:选择急性带状疱疹患者25例,随机分为药物治疗组(P组,n=13)和针灸治疗组(A组,n=12),选择12例正常人作为对照。P组采用抗病毒、营养神经和镇痛药物治疗,A组在相同药物治疗基础上加针灸治疗。比较两组患者治疗前、治疗后7 d VAS评分和皮疹愈合时间;Western-blot法检测c-FLIP表达,比较正常对照组与急性带状疱疹患者及两组患者治疗前、后血液c-FLIP表达情况。结果:两组患者治疗后VAS评分较治疗前均明显减少(P<0.01),治疗后组间VAS评分比较差异无统计学意义,A组皮疹愈合时间短于P组(P<0.01);急性带状疱疹患者c-FLIP表达比正常人表达明显降低(P<0.01),A组治疗后较治疗前表达升高(P<0.01),P组治疗前、后表达无明显变化。结论:针灸可以调节急性带状疱疹患者血液c-FLIP表达,促进皮疹愈合。这可能是针灸治疗带状疱疹的细胞免疫机制之一。
文摘用基因工程技术制备出的细粒棘球绦虫重组抗原 B( r Ag B)表达载体 ,经诱导表达、亲和层析纯化获得具生物活性的重组蛋白质 r Ag B,用 Western- Blot法检测病人血清。结果显示 r Ag B敏感性为 91.6% ( 4 4/48) ,特异性为 93 .8% ( 3 0 /3 2 ) ,其中 10例泡球蚴 ( AE)病人及 10例肿瘤病人血清均无交叉反应。说明 r Ag B具有较高的敏感性及特异性 ,可用于包虫病的常规血清学诊断 ,r Ag B在宿主菌 JM10 9内稳定表达 ,因此可在实验室内大量制备用于血清学诊断的 r Ag B。
基金the National Natural Science Foundation of China (30671293)the High-Tech Research and Development (863) Program of China(2006AA100102).
文摘The high molecular weight glutenin subunit （HMW-GS） pair 1Bx13＋1By16 are recognized to positively correlate with bread-making quality; however, their molecular data remain unknown. In order to reveal the mechanism by which 1By16 and 1Bx13 creates high quality, their open reading frames （ORFs） were amplified from common wheat Atlas66 and Jimai 20 using primers that were designed based on published sequences of HMW glutenin genes. The ORF of 1By16 was 2220bp, deduced into 738 amino acid residues with seven cysteines including 59 hexapeptides and 22 nanopeptides motifs. The ORF of 1Bx13 was 2385bp, deduced into 795 amino acid residues with four cysteines including 68 hexapeptides, 25 nanopeptides and six tripepUdes motifs. We found that 1By16 was the largest y-type HMW glutenin gene described to date in common wheat. The 1By16 had 36 amino acid residues inserted in the central repetitive domain compared with 1By15. Expression in bacteria and western-blot tests confirmed that the sequence cloned was the ORF of HMW-GS 1By16, and that 1Bx13 was one of the largest 1Bx genes that have been described so far in common wheat, exhibiting a hexapeptide （PGQGQQ） insertion in the end of central repetitive domain compared with 1Bx7. A phylogenetic tree based on the deduced full-length amino acid sequence alignment of the published HMW-GS genes showed that the 1By16 was clustered with Glu-1B-2, and that the 1Bx13 was clustered with Glu-1B-1 alleles.
文摘The proteins from endosperm, root, stem, leaf, leafstalk of Jatropha curcas L. and their calli were hybridized to the seed toxin protein (curcin) of Jatropha curcas L. by western blot. The result showed that the curcin was specifically expressed in the endosperm and its calli, while it was not detected in root, stem, leaf, and leafstalk of Jatropha curcas L. and their calli . This study indicated that calli induced from endosperm can be used to produce curcin.
