BACKGROUND Expression of the full-length isoform of Abelson interactor 1(ABI1),ABI1-p65,is increased in colorectal carcinoma(CRC)and is thought to be involved in one or more steps leading to tumor progression or metas...BACKGROUND Expression of the full-length isoform of Abelson interactor 1(ABI1),ABI1-p65,is increased in colorectal carcinoma(CRC)and is thought to be involved in one or more steps leading to tumor progression or metastasis.The ABI1 splice isoform-L(ABI1-SiL)has conserved WAVE2-binding and SH3 domains,lacks the homeodomain homologous region,and is missing the majority of PxxP-and Pro-rich domains found in full-length ABI1-p65.Thus,ABI1-SiL domain structure suggests that the protein may regulate CRC cell morphology,adhesion,migration,and metastasis via interactions with the WAVE2 complex pathway.AIM To investigate the potential role and underlying mechanisms associated with ABI1-SiL-mediated regulation of CRC.METHODS ABI1-SiL mRNA expression in CC tissue and cell lines was measured using both qualitative reverse transcriptase-polymerase chain reaction(RT-PCR)and realtime quantitative RT-PCR.A stably ABI1-SiL overexpressing SW480 cell model was constructed using Lipofectamine 2000,and cells selected with G418.Image J software,CCK8,and transwell assays were used to investigate SW480 cell surface area,proliferation,migration,and invasion.Immunoprecipitation,Western blot,and co-localization assays were performed to explore intermolecular interactions between ABI1-SiL,WAVE2,and ABI1-p65 proteins.RESULTS ABI1-SiL was expressed in normal colon tissue and was significantly decreased in CRC cell lines and tissues.Overexpression of ABI1-SiL in SW480 cells significantly increased the cell surface area and inhibited the adhesive and migration properties of the cells,but did not alter their invasive capacity.Similar to ABI1-p65,ABI1-SiL still binds WAVE2,and the ABI1-p65 isoform in SW480 cells.Furthermore,co-localization assays confirmed these intermolecular interactions.CONCLUSION These results support a model in which ABI1-SiL plays an anti-oncogenic role by competitively binding to WAVE2 and directly interacting with phosphorylated and non-phosphorylated ABI1-p65,functioning as a dominant-negative form of ABI1-p65.展开更多
【目的】将声触诊弹性成像(STE)和二维剪切波弹性成像(2D-SWE)检测肝脏硬度进行比较,为STE新技术的临床应用提供循证依据。【方法】采用STE和2D-SWE技术,对43例健康志愿者和63例肝硬化患者行肝硬度检测,对比分析两种技术的检测成功率、...【目的】将声触诊弹性成像(STE)和二维剪切波弹性成像(2D-SWE)检测肝脏硬度进行比较,为STE新技术的临床应用提供循证依据。【方法】采用STE和2D-SWE技术,对43例健康志愿者和63例肝硬化患者行肝硬度检测,对比分析两种技术的检测成功率、操作者内重复性、测值相关性和测值差异。【结果】肝脏STE、2D-SWE检测成功率分别为100%(106/106)、96.2%(102/106)(χ^2=2.320,P=0.128),4例2D-SWE检测失败的均为肝硬化患者。在健康组STE测值高于2D-SWE[5.83(5.30~6.37)kPa vs 5.00(4.80~5.20)kPa,Z=4.419,P<0.001]、肝硬化组低于2D-SWE[15.82(11.71~25.02)kPa vs 18.50(11.95~29.93)kPa,Z=-2.981,P=0.003]。STE和2D-SWE两种技术的测值相关系数为0.896(95%CI:0.849~0.928,P<0.001)。【结论】STE检测成功率高、重复性好,尤其在肝硬化患者穿透性好、与2D-SWE比较更具优势;STE测值与2D-SWE不直接等同,临床应用中需要注意。展开更多
基金National Natural Science Foundation of China,No.30872923 and No.81672853and Peking University People’s Hospital Scientific Research Development Found,No.RDH2020-11.
文摘BACKGROUND Expression of the full-length isoform of Abelson interactor 1(ABI1),ABI1-p65,is increased in colorectal carcinoma(CRC)and is thought to be involved in one or more steps leading to tumor progression or metastasis.The ABI1 splice isoform-L(ABI1-SiL)has conserved WAVE2-binding and SH3 domains,lacks the homeodomain homologous region,and is missing the majority of PxxP-and Pro-rich domains found in full-length ABI1-p65.Thus,ABI1-SiL domain structure suggests that the protein may regulate CRC cell morphology,adhesion,migration,and metastasis via interactions with the WAVE2 complex pathway.AIM To investigate the potential role and underlying mechanisms associated with ABI1-SiL-mediated regulation of CRC.METHODS ABI1-SiL mRNA expression in CC tissue and cell lines was measured using both qualitative reverse transcriptase-polymerase chain reaction(RT-PCR)and realtime quantitative RT-PCR.A stably ABI1-SiL overexpressing SW480 cell model was constructed using Lipofectamine 2000,and cells selected with G418.Image J software,CCK8,and transwell assays were used to investigate SW480 cell surface area,proliferation,migration,and invasion.Immunoprecipitation,Western blot,and co-localization assays were performed to explore intermolecular interactions between ABI1-SiL,WAVE2,and ABI1-p65 proteins.RESULTS ABI1-SiL was expressed in normal colon tissue and was significantly decreased in CRC cell lines and tissues.Overexpression of ABI1-SiL in SW480 cells significantly increased the cell surface area and inhibited the adhesive and migration properties of the cells,but did not alter their invasive capacity.Similar to ABI1-p65,ABI1-SiL still binds WAVE2,and the ABI1-p65 isoform in SW480 cells.Furthermore,co-localization assays confirmed these intermolecular interactions.CONCLUSION These results support a model in which ABI1-SiL plays an anti-oncogenic role by competitively binding to WAVE2 and directly interacting with phosphorylated and non-phosphorylated ABI1-p65,functioning as a dominant-negative form of ABI1-p65.
文摘【目的】将声触诊弹性成像(STE)和二维剪切波弹性成像(2D-SWE)检测肝脏硬度进行比较,为STE新技术的临床应用提供循证依据。【方法】采用STE和2D-SWE技术,对43例健康志愿者和63例肝硬化患者行肝硬度检测,对比分析两种技术的检测成功率、操作者内重复性、测值相关性和测值差异。【结果】肝脏STE、2D-SWE检测成功率分别为100%(106/106)、96.2%(102/106)(χ^2=2.320,P=0.128),4例2D-SWE检测失败的均为肝硬化患者。在健康组STE测值高于2D-SWE[5.83(5.30~6.37)kPa vs 5.00(4.80~5.20)kPa,Z=4.419,P<0.001]、肝硬化组低于2D-SWE[15.82(11.71~25.02)kPa vs 18.50(11.95~29.93)kPa,Z=-2.981,P=0.003]。STE和2D-SWE两种技术的测值相关系数为0.896(95%CI:0.849~0.928,P<0.001)。【结论】STE检测成功率高、重复性好,尤其在肝硬化患者穿透性好、与2D-SWE比较更具优势;STE测值与2D-SWE不直接等同,临床应用中需要注意。
