A high-performance liquid chromatography coupled ultraviolet （HPLC-UV） method was developed for a chemical fingerprint analysis of Viscum coloratura. Eighteen peaks were selected as the common peaks and Homoeriodict...A high-performance liquid chromatography coupled ultraviolet （HPLC-UV） method was developed for a chemical fingerprint analysis of Viscum coloratura. Eighteen peaks were selected as the common peaks and Homoeriodictyol-7-O-β-D-apiosiyl-（1→2）-β-D-glucoside was used as a reference. The relative areas of common peaks were used for hierarchical clustering analysis and similarity calculation. Thirty-seven samples collected from different sources were classified into five groups. The similarities of 21 batches Viscum coloratura samples were beyond 0.90. The results obtained suggest that the chromatographic fingerprint can efficiently identify Viscum coloratum. Additionally, the fingerprints can then be used to evaluate the correlation between Viscum coloratura and hosts.展开更多
A novel polypeptide was isolated from mistletoe Viscum coloratum. The primary structure of the polypeptide named viscotoxin B2 was determined to be KSCCKNTTGRNIYNT CRFAGGSRERCAKLSGCKIISASTCPSDYPK by Edman degradation....A novel polypeptide was isolated from mistletoe Viscum coloratum. The primary structure of the polypeptide named viscotoxin B2 was determined to be KSCCKNTTGRNIYNT CRFAGGSRERCAKLSGCKIISASTCPSDYPK by Edman degradation. Viscotoxin B2 shared high sequence homology with viscotoxins isolated from Viscum album. Pharmacological experiments showed that viscotoxin B2 had distinct cytotoxic activity on tumor cells. Viscotoxin B2 could be used as a leading compound in cancer therapy.展开更多
In January 2017, symptoms of a leaf-spot disease were observed on Viscum coloratum plants in Yichun,China. The infected leaves were chlorotic, while sunken lesions formed on diseased branches, which were initially lig...In January 2017, symptoms of a leaf-spot disease were observed on Viscum coloratum plants in Yichun,China. The infected leaves were chlorotic, while sunken lesions formed on diseased branches, which were initially light brown and later turned dark brown. Moreover, diseased branches and leaves formed semi-buried, small, and spherical pycnidia. In total, 20 leaves and 19 branches diseased samples from Yichun, China were collected and examined. The fungus was isolated and identified using Koch’s postulates, morphological characteristics and DNA sequence data. The data presented herein confirmed that the pathogen responsible for the disease was Sphaeropsis visci. To the best of our knowledge, this is the first report of it in China.展开更多
基金supported by the National NaturalScience Foundation of China(No.30901967)the Scientific Research Foundation for the Doctors of the Liaoning Provincial Department of Science and Technology(No.20091078)the Science and Technology Research Projects of the Educational Commission of Liaoning Province(No.2009A684)for financial support
文摘A high-performance liquid chromatography coupled ultraviolet （HPLC-UV） method was developed for a chemical fingerprint analysis of Viscum coloratura. Eighteen peaks were selected as the common peaks and Homoeriodictyol-7-O-β-D-apiosiyl-（1→2）-β-D-glucoside was used as a reference. The relative areas of common peaks were used for hierarchical clustering analysis and similarity calculation. Thirty-seven samples collected from different sources were classified into five groups. The similarities of 21 batches Viscum coloratura samples were beyond 0.90. The results obtained suggest that the chromatographic fingerprint can efficiently identify Viscum coloratum. Additionally, the fingerprints can then be used to evaluate the correlation between Viscum coloratura and hosts.
文摘A novel polypeptide was isolated from mistletoe Viscum coloratum. The primary structure of the polypeptide named viscotoxin B2 was determined to be KSCCKNTTGRNIYNT CRFAGGSRERCAKLSGCKIISASTCPSDYPK by Edman degradation. Viscotoxin B2 shared high sequence homology with viscotoxins isolated from Viscum album. Pharmacological experiments showed that viscotoxin B2 had distinct cytotoxic activity on tumor cells. Viscotoxin B2 could be used as a leading compound in cancer therapy.
基金supported by the forestry public welfare industry research special-study group on cultivation and utilization of medicinal fungi and astragalus in Daxing’anling(201004079)
文摘In January 2017, symptoms of a leaf-spot disease were observed on Viscum coloratum plants in Yichun,China. The infected leaves were chlorotic, while sunken lesions formed on diseased branches, which were initially light brown and later turned dark brown. Moreover, diseased branches and leaves formed semi-buried, small, and spherical pycnidia. In total, 20 leaves and 19 branches diseased samples from Yichun, China were collected and examined. The fungus was isolated and identified using Koch’s postulates, morphological characteristics and DNA sequence data. The data presented herein confirmed that the pathogen responsible for the disease was Sphaeropsis visci. To the best of our knowledge, this is the first report of it in China.
