To evaluate prospectively 4 selected serum fibrosis markers （tenascin, hyaluronan, collagen Ⅵ, TIMP-1） before, during and 12 mo after IFN treatment of children with chronic hepatitis B. METHODS： Forty-seven consec...To evaluate prospectively 4 selected serum fibrosis markers （tenascin, hyaluronan, collagen Ⅵ, TIMP-1） before, during and 12 mo after IFN treatment of children with chronic hepatitis B. METHODS： Forty-seven consecutive patients with chronic hepatitis B （range 4-16 years, mean 8 years） underwent IFN treatment （3 MU tiw for 20 wk）. Fibrosis stage and inflammation grade were assessed in a blinded fashion before and 12 mo after end of treatment. Serum fibrosis markers were determined using automated assays.RESULTS： IFN treatment improved histological inflammation but did not change fibrosis in the whole group or in subgroups. Only hyaluronan correlated significantly with histological fibrosis（r = 0.3383, P = 0.022）. Basal fibrosis markers did not differ between responders （42.5%） and nonresponders（57.5%）. During IFN treatment only serum tenascin decreased significantly in the whole group and in nonresponders. When pretreatment values were compared to values 12 mo after therapy, TIMP-1 increased in all patients and in nonresponders, and hyaluronan decreased in all patients and in responders.CONCLUSION： Tenascin reflects hepatic fibrogenesis and inflammation which decreases during IFN treatment of children with chronic hepatitis B. TIMP-1 correlates with nonresponse and hyaluronan with histological fibrosis.展开更多
When pigs are infected with classical swine fever virus （ CSFV）, the antibody primarily targets the structural giycoprotein E^rns of the virus. Previous investigations have demonstrated that ETM has low or no virus...When pigs are infected with classical swine fever virus （ CSFV）, the antibody primarily targets the structural giycoprotein E^rns of the virus. Previous investigations have demonstrated that ETM has low or no virus neutralizing capacity. In this study, candidate subunit marker vaccine, chaperonin 10 （Cpnl0）-E^rns, which possess the property of generating neutralized antibodies against lethal challenge of virulent CSFV was developed. The gene of ETM was isolated from Hog cholera lapinized virus （HCLV） -infected spleen cells of rabbits via RT-PCR method and fused to the downstream region of the cpn10 gene; the products of recombinant fusion protein （ cpn10-E^rns ） induced expression in Escherichia coli, and the products were purified by affinity chromatography. During the course of vaccination, the candidate vaccines cpn10-E^rns were used for the immunization of guinea pigs, and they induced a strong antibody response against cpn10-E^rns. The antibodies can be immobilized by coating inactivated CSFV particles, indicating that these antibodies can recognize CSFV. Neutralization assay was carried out on rabbits according to National Regulations on Veterinary Drug. The results clearly indicate that the typical fever of rabbits induced by the live attenuated HCLV could be inhibited by preincubation with the antisera （dilution 1： 4） induced by cpn10-E^rns, but not inhibited by preincubation with the antisera induced only by E^rns. Analogous results were observed for the group of the rabbits immunized with cpn10-E^rns, which were protected against the typical fever induced by the challenge with HCLV. The findings of this study formed the basis of a new means for developing subunit marker vaccine against CSFV.展开更多
基金Supported by the Interdisciplinary Center for Clinical Research(IZKF) of the University of Erlangen-Nuernberg, Germany
文摘To evaluate prospectively 4 selected serum fibrosis markers （tenascin, hyaluronan, collagen Ⅵ, TIMP-1） before, during and 12 mo after IFN treatment of children with chronic hepatitis B. METHODS： Forty-seven consecutive patients with chronic hepatitis B （range 4-16 years, mean 8 years） underwent IFN treatment （3 MU tiw for 20 wk）. Fibrosis stage and inflammation grade were assessed in a blinded fashion before and 12 mo after end of treatment. Serum fibrosis markers were determined using automated assays.RESULTS： IFN treatment improved histological inflammation but did not change fibrosis in the whole group or in subgroups. Only hyaluronan correlated significantly with histological fibrosis（r = 0.3383, P = 0.022）. Basal fibrosis markers did not differ between responders （42.5%） and nonresponders（57.5%）. During IFN treatment only serum tenascin decreased significantly in the whole group and in nonresponders. When pretreatment values were compared to values 12 mo after therapy, TIMP-1 increased in all patients and in nonresponders, and hyaluronan decreased in all patients and in responders.CONCLUSION： Tenascin reflects hepatic fibrogenesis and inflammation which decreases during IFN treatment of children with chronic hepatitis B. TIMP-1 correlates with nonresponse and hyaluronan with histological fibrosis.
基金Supported by the National Key Basic Research Program of China(No. 2001CB510007).
文摘When pigs are infected with classical swine fever virus （ CSFV）, the antibody primarily targets the structural giycoprotein E^rns of the virus. Previous investigations have demonstrated that ETM has low or no virus neutralizing capacity. In this study, candidate subunit marker vaccine, chaperonin 10 （Cpnl0）-E^rns, which possess the property of generating neutralized antibodies against lethal challenge of virulent CSFV was developed. The gene of ETM was isolated from Hog cholera lapinized virus （HCLV） -infected spleen cells of rabbits via RT-PCR method and fused to the downstream region of the cpn10 gene; the products of recombinant fusion protein （ cpn10-E^rns ） induced expression in Escherichia coli, and the products were purified by affinity chromatography. During the course of vaccination, the candidate vaccines cpn10-E^rns were used for the immunization of guinea pigs, and they induced a strong antibody response against cpn10-E^rns. The antibodies can be immobilized by coating inactivated CSFV particles, indicating that these antibodies can recognize CSFV. Neutralization assay was carried out on rabbits according to National Regulations on Veterinary Drug. The results clearly indicate that the typical fever of rabbits induced by the live attenuated HCLV could be inhibited by preincubation with the antisera （dilution 1： 4） induced by cpn10-E^rns, but not inhibited by preincubation with the antisera induced only by E^rns. Analogous results were observed for the group of the rabbits immunized with cpn10-E^rns, which were protected against the typical fever induced by the challenge with HCLV. The findings of this study formed the basis of a new means for developing subunit marker vaccine against CSFV.
