BACKGROUND: Angiogenesis is known to be essential to the survival, growth, invasion, and metastasis of tumor cells. Vascular endothelial growth factor (VEGF) are an important angiogenic factor regulating tumor angioge...BACKGROUND: Angiogenesis is known to be essential to the survival, growth, invasion, and metastasis of tumor cells. Vascular endothelial growth factor (VEGF) are an important angiogenic factor regulating tumor angiogenesis, but its significance and tumor pathologic features are un- clear in hepatocellular carcinoma (HCC). In the present study, we analyzed expression of tissue VEGF, alteration of microvascular density (MVD) in microvessel angiogenesis, development and metastasis of HCC, and level of serum VEGF in differential diagnosis of benign and malignant liv- er diseases. METHODS: Tumor specimens were prospectively collected from HCC patients undergoing resection. Total RNAs were extracted and the expression levels were detected from different parts of HCC tissues. The cellular distributions of VEGF and MVD of liver tumors and their paracancerous and distal cancerous tissues were investigated by streptavi- din peroxidase (S-P) immunohistochemistry, respectively. The VEGF levels of circulating blood and hepatoma tissues were measured by enzyme-linked immunosorbent assay. RESULTS: The incidence of VEGF expression was 63.9% in HCCs (23/36 cases), 78.3% in non-encapsulated HCCs (18/23), and 90.9% in HCCs with extrahepatic metastasis (10/11), respectively. The VEGF expression was tightly correlated with MVD (P <0.01). The MVD in HCC with metastasis, low differentiation or non-encapsulation was significantly higher than that in HCC with intact capsule, high differentiation, or no metastasis. No significant diffe- rence was found between VEGF, MVD, tumor size, and hepatitis virus infection. The level of total RNA in HCC tis- sues was significantly lower but the VEGF level significantly higher than those in paracancerous or distal cancerous ones (P<0.01). The abnormal expression levels of VEGF in sera of HCC patients were directly correlated with the me- tastasis and recurrence of tumors. CONCLUSION: The high expression of VEGF and abnor- mality of tissue MVD are useful predictors for vascular inva- sion and metastas展开更多
目的观察肛痈促愈汤在促进肛周脓肿患者术后创面愈合的作用及对血管内皮因子的影响。方法选择2018年1月—2019年8月医院收治的肛周脓肿患者83例,依照治疗方案的不同分为观察组(42例)和对照组(41例)。两组患者均采用一次性肛周脓肿根治...目的观察肛痈促愈汤在促进肛周脓肿患者术后创面愈合的作用及对血管内皮因子的影响。方法选择2018年1月—2019年8月医院收治的肛周脓肿患者83例,依照治疗方案的不同分为观察组(42例)和对照组(41例)。两组患者均采用一次性肛周脓肿根治术治疗。对照组采用高猛酸钾坐浴,患者大便后清洗创面,观察组采用肛痈促愈汤辅助治疗,直至创面愈合。比较两组治疗疗效,两组患者术后不同时间点症状及体征评分;比较两组腐肉完全脱离时间、创面愈合时间和创面缩小率;比较血清VEGF、IL-1β、TNF-α和IL-6含量;比较两组不同时段肛门功能。结果观察组总有效率为90.48%(38/42),对照组治疗总有效率为73.17%(30/41),观察组治疗总有效率高于对照组(P<0.05);观察组术后7 d和术后14 d肛门疼痛、肛门周围组织水肿、分泌物和肉芽形态的评分低于对照组(P<0.05);观察组患者腐肉完全脱离时间、创面愈合时间和创面缩小率均低于对照组(P<0.05);观察组术后14 d VEGF水平高于对照组,炎性细胞因子IL-6、IL-8和TNF-α水平低于对照组(P<0.05);观察组术后1个月和术后3个月肛管静息压和肛管最大收缩压均高于对照组(P<0.05)。结论肛痈促愈汤可促进肛周脓肿患者一次性根治术后创面愈合和肉芽生长,提高血清VEGF水平,降低炎症反应,有效恢复肛门收缩功能,提高临床总有效率。展开更多
基金This study was supported in part by grant from the KeyProject Foundation of Medical Sciences of Jiangsu province(RC2003100)
文摘BACKGROUND: Angiogenesis is known to be essential to the survival, growth, invasion, and metastasis of tumor cells. Vascular endothelial growth factor (VEGF) are an important angiogenic factor regulating tumor angiogenesis, but its significance and tumor pathologic features are un- clear in hepatocellular carcinoma (HCC). In the present study, we analyzed expression of tissue VEGF, alteration of microvascular density (MVD) in microvessel angiogenesis, development and metastasis of HCC, and level of serum VEGF in differential diagnosis of benign and malignant liv- er diseases. METHODS: Tumor specimens were prospectively collected from HCC patients undergoing resection. Total RNAs were extracted and the expression levels were detected from different parts of HCC tissues. The cellular distributions of VEGF and MVD of liver tumors and their paracancerous and distal cancerous tissues were investigated by streptavi- din peroxidase (S-P) immunohistochemistry, respectively. The VEGF levels of circulating blood and hepatoma tissues were measured by enzyme-linked immunosorbent assay. RESULTS: The incidence of VEGF expression was 63.9% in HCCs (23/36 cases), 78.3% in non-encapsulated HCCs (18/23), and 90.9% in HCCs with extrahepatic metastasis (10/11), respectively. The VEGF expression was tightly correlated with MVD (P <0.01). The MVD in HCC with metastasis, low differentiation or non-encapsulation was significantly higher than that in HCC with intact capsule, high differentiation, or no metastasis. No significant diffe- rence was found between VEGF, MVD, tumor size, and hepatitis virus infection. The level of total RNA in HCC tis- sues was significantly lower but the VEGF level significantly higher than those in paracancerous or distal cancerous ones (P<0.01). The abnormal expression levels of VEGF in sera of HCC patients were directly correlated with the me- tastasis and recurrence of tumors. CONCLUSION: The high expression of VEGF and abnor- mality of tissue MVD are useful predictors for vascular inva- sion and metastas
文摘目的观察肛痈促愈汤在促进肛周脓肿患者术后创面愈合的作用及对血管内皮因子的影响。方法选择2018年1月—2019年8月医院收治的肛周脓肿患者83例,依照治疗方案的不同分为观察组(42例)和对照组(41例)。两组患者均采用一次性肛周脓肿根治术治疗。对照组采用高猛酸钾坐浴,患者大便后清洗创面,观察组采用肛痈促愈汤辅助治疗,直至创面愈合。比较两组治疗疗效,两组患者术后不同时间点症状及体征评分;比较两组腐肉完全脱离时间、创面愈合时间和创面缩小率;比较血清VEGF、IL-1β、TNF-α和IL-6含量;比较两组不同时段肛门功能。结果观察组总有效率为90.48%(38/42),对照组治疗总有效率为73.17%(30/41),观察组治疗总有效率高于对照组(P<0.05);观察组术后7 d和术后14 d肛门疼痛、肛门周围组织水肿、分泌物和肉芽形态的评分低于对照组(P<0.05);观察组患者腐肉完全脱离时间、创面愈合时间和创面缩小率均低于对照组(P<0.05);观察组术后14 d VEGF水平高于对照组,炎性细胞因子IL-6、IL-8和TNF-α水平低于对照组(P<0.05);观察组术后1个月和术后3个月肛管静息压和肛管最大收缩压均高于对照组(P<0.05)。结论肛痈促愈汤可促进肛周脓肿患者一次性根治术后创面愈合和肉芽生长,提高血清VEGF水平,降低炎症反应,有效恢复肛门收缩功能,提高临床总有效率。
