Objective To compare the diagnostic efficiency of the thyroid imaging reporting and data system(TIRADS),the Bethesda system for reporting thyroid cytopathology(BSRTC)and BRAFV600Edetection,and their combined use in th...Objective To compare the diagnostic efficiency of the thyroid imaging reporting and data system(TIRADS),the Bethesda system for reporting thyroid cytopathology(BSRTC)and BRAFV600Edetection,and their combined use in the differentiation between benign and malignant thyroid nodules.Methods One hundred展开更多
Src regulates cell adhesion, invasiveness, motility and growth in cancer cells. In melanoma, accumulating data show that Src inhibition can be effective and may enhance the effects of other agents. Increased Src expre...Src regulates cell adhesion, invasiveness, motility and growth in cancer cells. In melanoma, accumulating data show that Src inhibition can be effective and may enhance the effects of other agents. Increased Src expression and activity thus has recently become a target for drug therapy. Several melanoma cell lines were exposed to inhibitors of Src activity despite their broad specificity. To examine the particular activity of Src in human melanoma cells, we used SU6656, the selective inhibitor of Src family protein kinases. The activity of Src and cell proliferation were suppressed in HBL human cells, wild type melanoma cells and in SK-MEL-5 human melanoma cells harboring mutant BRAF V600E, upon their treatment with SU6656. The suppression of Src kinase activity had not inhibitory effects on Akt/PKB activity in SK-MEL-5 cells, which we have previously found in HBL cells. This may indicate that changes of Src involvement in the control of Akt/PKB activity and its downstream signaling could be induced by BRAF V600E mutation in SK-MEL-5 cells.展开更多
目的探讨超声造影(contrast-enhanced ultrasound,CEUS)联合丝/苏氨酸特异性激酶(serine-threonine-threonine protein kinase,BRAF)基因V600E(BRAFV600E)突变检测对细胞学意义不明确类型结节的诊断价值。方法回顾性分析129个甲状腺超...目的探讨超声造影(contrast-enhanced ultrasound,CEUS)联合丝/苏氨酸特异性激酶(serine-threonine-threonine protein kinase,BRAF)基因V600E(BRAFV600E)突变检测对细胞学意义不明确类型结节的诊断价值。方法回顾性分析129个甲状腺超声引导下细针穿刺意义不明结节的常规超声检查、CEUS及BRAFV600E基因检测的资料,以BRAFV600E阳性和CEUS不均匀低增强等表现作为甲状腺结节恶性诊断标准,并与术后病理学结果做对照,绘制ROC曲线,比较CEUS、BRAFV600E基因检测及CEUS联合BRAFV600E基因检测对该类结节良恶性的鉴别诊断价值。结果CEUS、BRAFV600E基因检测对细针穿刺细胞学意义不明确结节诊断良恶性的敏感性、特异性、准确性分别为86.7%、83.3%、85.3%和72.0%、100%、83.7%。CEUS对细针穿刺细胞学意义不明确结节诊断良恶性的敏感性和准确性高于BRAFV600E基因检测,差异有统计学意义(均P<0.001),而其特异性低于BRAFV600E(P<0.001)。两种方法联合诊断该类结节良恶性的敏感性、特异性、准确性分别为94.7%、83.3%、89.9%,联合诊断效率最高,差异有统计学意义(P<0.001),其ROC曲线下面积(AUC)高于BRAFV600E基因检测及CEUS检查(0.951对0.860、0.901)。结论CEUS联合BRAFV600E基因检测有助于提高鉴别甲状腺超声引导下细针穿刺意义不明结节病灶良恶性的准确性。展开更多
文摘Objective To compare the diagnostic efficiency of the thyroid imaging reporting and data system(TIRADS),the Bethesda system for reporting thyroid cytopathology(BSRTC)and BRAFV600Edetection,and their combined use in the differentiation between benign and malignant thyroid nodules.Methods One hundred
基金supported by grant NT11231-3/2010 from the Ministry of Health of the Czech Republic
文摘Src regulates cell adhesion, invasiveness, motility and growth in cancer cells. In melanoma, accumulating data show that Src inhibition can be effective and may enhance the effects of other agents. Increased Src expression and activity thus has recently become a target for drug therapy. Several melanoma cell lines were exposed to inhibitors of Src activity despite their broad specificity. To examine the particular activity of Src in human melanoma cells, we used SU6656, the selective inhibitor of Src family protein kinases. The activity of Src and cell proliferation were suppressed in HBL human cells, wild type melanoma cells and in SK-MEL-5 human melanoma cells harboring mutant BRAF V600E, upon their treatment with SU6656. The suppression of Src kinase activity had not inhibitory effects on Akt/PKB activity in SK-MEL-5 cells, which we have previously found in HBL cells. This may indicate that changes of Src involvement in the control of Akt/PKB activity and its downstream signaling could be induced by BRAF V600E mutation in SK-MEL-5 cells.
文摘目的探讨超声造影(contrast-enhanced ultrasound,CEUS)联合丝/苏氨酸特异性激酶(serine-threonine-threonine protein kinase,BRAF)基因V600E(BRAFV600E)突变检测对细胞学意义不明确类型结节的诊断价值。方法回顾性分析129个甲状腺超声引导下细针穿刺意义不明结节的常规超声检查、CEUS及BRAFV600E基因检测的资料,以BRAFV600E阳性和CEUS不均匀低增强等表现作为甲状腺结节恶性诊断标准,并与术后病理学结果做对照,绘制ROC曲线,比较CEUS、BRAFV600E基因检测及CEUS联合BRAFV600E基因检测对该类结节良恶性的鉴别诊断价值。结果CEUS、BRAFV600E基因检测对细针穿刺细胞学意义不明确结节诊断良恶性的敏感性、特异性、准确性分别为86.7%、83.3%、85.3%和72.0%、100%、83.7%。CEUS对细针穿刺细胞学意义不明确结节诊断良恶性的敏感性和准确性高于BRAFV600E基因检测,差异有统计学意义(均P<0.001),而其特异性低于BRAFV600E(P<0.001)。两种方法联合诊断该类结节良恶性的敏感性、特异性、准确性分别为94.7%、83.3%、89.9%,联合诊断效率最高,差异有统计学意义(P<0.001),其ROC曲线下面积(AUC)高于BRAFV600E基因检测及CEUS检查(0.951对0.860、0.901)。结论CEUS联合BRAFV600E基因检测有助于提高鉴别甲状腺超声引导下细针穿刺意义不明结节病灶良恶性的准确性。
