Recent studies suggest that Hypocretin (HCRT, Orexin) are involved in stress regulation of depression through the hypothalamic-pituitary-adrenal (HPA) axis. However, the molecular mechanism by which Hypocretin regulat...Recent studies suggest that Hypocretin (HCRT, Orexin) are involved in stress regulation of depression through the hypothalamic-pituitary-adrenal (HPA) axis. However, the molecular mechanism by which Hypocretin regulate neurobiological responses is unknown. Herein, the effects of chronic stress on the epigenetic modification of HCRT and its association with depression were explored with regard to a potential role in cancer progression. In the study, Sprague Dawley (SD) rats were used to establish an animal model of cancer with depression by administrating n-nitrosodiethylamine (DEN) and chronic unpredictable mild stress (CUMS). RNA-sequencing was used to detect differentially expressed genes in the hippocampus of rats and quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the results of RNA-sequencing. The status of HCRT promoter methylation was assessed by methylation specific polymerase chain reaction. Behavioral tests showed that rats exposed to CUMS had significant depressive-like behaviors. The number of liver tumors and tumor load in depressed rats exposed to CUMS was higher than in SD rats without CUMS. RNA-sequencing revealed that HCRT was one of the most siginificantly downregulated gene in the hippocampus of SD rats with CUMS compared to non-stressed group, which was validated by qRT-PCR. HCRT mRNA expression was downregulated and the promoter for HCRT was hyper-methylated in those with depression. These results identified a critical role for chronic psychological stressors in tumorigenesis and cancer progression, via epigenetic HCRT downregulation. Such epigenetic downregulation may be the molecular basis for the association of cancer with depression.展开更多
Digital twin is an essential enabling technology for 6G connected vehicles.Through highfidelity mobility simulation,digital twin is expected to make accurate prediction about the vehicle trajectory,and then support in...Digital twin is an essential enabling technology for 6G connected vehicles.Through highfidelity mobility simulation,digital twin is expected to make accurate prediction about the vehicle trajectory,and then support intelligent applications such as safety monitoring and self-driving for connected vehicles.However,it is observed that even if a digital twin model is perfectly derived,it might still fail to predict the trajectory due to tiny measurement noise or delay in the initial vehicle locations.This paper aims at investigating the sources of unpredictability of digital twin.Take the car-following behaviors in connected vehicles for case study.The theoretical analysis and experimental results indicate that the predictability of digital twin naturally depends on its system complexity.Once a system enters a complex pattern,its longterm states are unpredictable.Furthermore,our study discloses that the complexity is determined,on the one hand,by the intrinsic factors of the target physical system such as the driver’s response sensitivity and delay,and on the other hand,by the crucial parameters of the digital twin system such as the sampling interval and twining latency.展开更多
Bloom’s taxonomy is widely used in educational research to categorize the cognitive skills required to answer exam questions.For this study,we analyzed how students categorize exam questions(high-level question or lo...Bloom’s taxonomy is widely used in educational research to categorize the cognitive skills required to answer exam questions.For this study,we analyzed how students categorize exam questions(high-level question or low-level question,)gathered data as to their rationale for categorization,and compared their categorizations to those of experts.We found that students consistently rank high-level questions incorrectly.We analyzed student reasons for their categorizations,and found that for many of the incorrectly categorized questions the students referred to reasons related to Cognitive Load Theory.This shows that cognitive load prevents students from accurately assessing the cognitive level of an exam question.Thus,extra cognitive load in exam questions may prevent those questions from accurately measuring the skills and knowledge of the student.This points to the need for instructors to eliminate cognitive load from their exams.展开更多
基金The study was supported by the National Natural Science Foundation of China (NSFC) (No.#81872380)the Opening Foundation of Chongqing Key Laboratory of Molecular Oncology and Epigenetics.
文摘Recent studies suggest that Hypocretin (HCRT, Orexin) are involved in stress regulation of depression through the hypothalamic-pituitary-adrenal (HPA) axis. However, the molecular mechanism by which Hypocretin regulate neurobiological responses is unknown. Herein, the effects of chronic stress on the epigenetic modification of HCRT and its association with depression were explored with regard to a potential role in cancer progression. In the study, Sprague Dawley (SD) rats were used to establish an animal model of cancer with depression by administrating n-nitrosodiethylamine (DEN) and chronic unpredictable mild stress (CUMS). RNA-sequencing was used to detect differentially expressed genes in the hippocampus of rats and quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the results of RNA-sequencing. The status of HCRT promoter methylation was assessed by methylation specific polymerase chain reaction. Behavioral tests showed that rats exposed to CUMS had significant depressive-like behaviors. The number of liver tumors and tumor load in depressed rats exposed to CUMS was higher than in SD rats without CUMS. RNA-sequencing revealed that HCRT was one of the most siginificantly downregulated gene in the hippocampus of SD rats with CUMS compared to non-stressed group, which was validated by qRT-PCR. HCRT mRNA expression was downregulated and the promoter for HCRT was hyper-methylated in those with depression. These results identified a critical role for chronic psychological stressors in tumorigenesis and cancer progression, via epigenetic HCRT downregulation. Such epigenetic downregulation may be the molecular basis for the association of cancer with depression.
基金supported in part by National Key R&D Program of China (No.2020YFB1807802)National Natural Science Foundation of China (Nos.61971148,U22A2054)。
文摘Digital twin is an essential enabling technology for 6G connected vehicles.Through highfidelity mobility simulation,digital twin is expected to make accurate prediction about the vehicle trajectory,and then support intelligent applications such as safety monitoring and self-driving for connected vehicles.However,it is observed that even if a digital twin model is perfectly derived,it might still fail to predict the trajectory due to tiny measurement noise or delay in the initial vehicle locations.This paper aims at investigating the sources of unpredictability of digital twin.Take the car-following behaviors in connected vehicles for case study.The theoretical analysis and experimental results indicate that the predictability of digital twin naturally depends on its system complexity.Once a system enters a complex pattern,its longterm states are unpredictable.Furthermore,our study discloses that the complexity is determined,on the one hand,by the intrinsic factors of the target physical system such as the driver’s response sensitivity and delay,and on the other hand,by the crucial parameters of the digital twin system such as the sampling interval and twining latency.
文摘Bloom’s taxonomy is widely used in educational research to categorize the cognitive skills required to answer exam questions.For this study,we analyzed how students categorize exam questions(high-level question or low-level question,)gathered data as to their rationale for categorization,and compared their categorizations to those of experts.We found that students consistently rank high-level questions incorrectly.We analyzed student reasons for their categorizations,and found that for many of the incorrectly categorized questions the students referred to reasons related to Cognitive Load Theory.This shows that cognitive load prevents students from accurately assessing the cognitive level of an exam question.Thus,extra cognitive load in exam questions may prevent those questions from accurately measuring the skills and knowledge of the student.This points to the need for instructors to eliminate cognitive load from their exams.
