The trehalose 6 phosphate synthase gene tps 1 was amplified from yeast S.cerevisiae AS2.1416 cDNA library by polymerase chain reaction.This 1.5 kb fragment was cloned into Pst I and Bam HI sites of pGEM T easy vector ...The trehalose 6 phosphate synthase gene tps 1 was amplified from yeast S.cerevisiae AS2.1416 cDNA library by polymerase chain reaction.This 1.5 kb fragment was cloned into Pst I and Bam HI sites of pGEM T easy vector and the sequence of the gene indicated the cloned tps 1 gene contained 1485 nucleotides encoding for 495 amino acid and shared a sequence homology of 99.6% with that from S.cerevisiae S288C.展开更多
Trehalose plays an important role in protecting organisms from various stresses. Trehalose-6-phosphate synthase （TPS） is the key enzyme in trehalose synthesis, but in insects only a few TPS genes have been identifie...Trehalose plays an important role in protecting organisms from various stresses. Trehalose-6-phosphate synthase （TPS） is the key enzyme in trehalose synthesis, but in insects only a few TPS genes have been identified and their function has not been well characterized. To better understand the function of TPS in insects, a complete TPS complementary DNA （eDNA） clone was obtained from the fat body of the locust Locusta migratoria manilensis （GenBank accession number： EU131894）. The full-length cDNA is 2 806 bp, including an open reading frame of 2 442 bp, which encodes an 813 amino acids protein with a calculated molecular weight of 91 976 Daltons and an isoelectric point of 6.14. The deduced amino acid sequence is highly similar to other published insect TPS and its C-terminal also has a region homologous to trehalose phosphate phsophatase （TPP）. Semi-quantitative analysis indicated that the TPS transcript was expressed not only in fat body, but also in gut, hemolymph and leg muscle. These data may facilitate studies of TPS function in insects and improve our understanding of trehalose metabolism.展开更多
文摘The trehalose 6 phosphate synthase gene tps 1 was amplified from yeast S.cerevisiae AS2.1416 cDNA library by polymerase chain reaction.This 1.5 kb fragment was cloned into Pst I and Bam HI sites of pGEM T easy vector and the sequence of the gene indicated the cloned tps 1 gene contained 1485 nucleotides encoding for 495 amino acid and shared a sequence homology of 99.6% with that from S.cerevisiae S288C.
文摘Trehalose plays an important role in protecting organisms from various stresses. Trehalose-6-phosphate synthase （TPS） is the key enzyme in trehalose synthesis, but in insects only a few TPS genes have been identified and their function has not been well characterized. To better understand the function of TPS in insects, a complete TPS complementary DNA （eDNA） clone was obtained from the fat body of the locust Locusta migratoria manilensis （GenBank accession number： EU131894）. The full-length cDNA is 2 806 bp, including an open reading frame of 2 442 bp, which encodes an 813 amino acids protein with a calculated molecular weight of 91 976 Daltons and an isoelectric point of 6.14. The deduced amino acid sequence is highly similar to other published insect TPS and its C-terminal also has a region homologous to trehalose phosphate phsophatase （TPP）. Semi-quantitative analysis indicated that the TPS transcript was expressed not only in fat body, but also in gut, hemolymph and leg muscle. These data may facilitate studies of TPS function in insects and improve our understanding of trehalose metabolism.
