The test of deep freezing (-196℃) preservation of mammalian embryos was applied successfully to mouse (for the first time) in 1972 (Whittingham, Leibo and Mazur, 1972), and subsequently to cattle (Wilmut and Rowson, ...The test of deep freezing (-196℃) preservation of mammalian embryos was applied successfully to mouse (for the first time) in 1972 (Whittingham, Leibo and Mazur, 1972), and subsequently to cattle (Wilmut and Rowson, 1973), sheep (Willad-展开更多
[Objectives] This study was conducted to investigate the possibility of screening for herbicide resistance in the early stage of rapes growth. [Methods] Eight treatments were set for a herbicide concentration experime...[Objectives] This study was conducted to investigate the possibility of screening for herbicide resistance in the early stage of rapes growth. [Methods] Eight treatments were set for a herbicide concentration experiment. [Results] Rape sprouts were highly sensitive to the herbicide glyphosate, and even a very low concentration of glyphosate solution(187 mg/L) was sufficient to kill most of the rape seedlings, while the genetically modified herbicide-tolerant rape could tolerate higher concentrations of the herbicide. Low concentrations of glyphosate could be used for screening at the sprout stage, and the results of herbicide-tolerance screening were consistent with PCR testing. [Conclusions] This study lays a technical and material foundation for accelerating the cultivation of new herbicide-resistant rape varieties suitable for rapeseed production areas in the lower reaches of the Yangtze River.展开更多
基金This research was supported in part by Jia Jingxiao (贾敬肖), Shanxi Institute of Zoology
文摘The test of deep freezing (-196℃) preservation of mammalian embryos was applied successfully to mouse (for the first time) in 1972 (Whittingham, Leibo and Mazur, 1972), and subsequently to cattle (Wilmut and Rowson, 1973), sheep (Willad-
基金Supported by National Science and Technology Major Project (2018ZX08020001-006)。
文摘[Objectives] This study was conducted to investigate the possibility of screening for herbicide resistance in the early stage of rapes growth. [Methods] Eight treatments were set for a herbicide concentration experiment. [Results] Rape sprouts were highly sensitive to the herbicide glyphosate, and even a very low concentration of glyphosate solution(187 mg/L) was sufficient to kill most of the rape seedlings, while the genetically modified herbicide-tolerant rape could tolerate higher concentrations of the herbicide. Low concentrations of glyphosate could be used for screening at the sprout stage, and the results of herbicide-tolerance screening were consistent with PCR testing. [Conclusions] This study lays a technical and material foundation for accelerating the cultivation of new herbicide-resistant rape varieties suitable for rapeseed production areas in the lower reaches of the Yangtze River.