期刊文献+
共找到5篇文章
< 1 >
每页显示 20 50 100
弓首蛔虫及狮弓蛔虫线粒体基因组nad4基因多态性研究 被引量:6
1
作者 李明伟 林瑞庆 +4 位作者 曹湛 魏冬霞 邹丰才 宋慧群 朱兴全 《热带医学杂志》 CAS 2006年第3期236-239,250,共5页
目的比较犬、猫常见蛔虫的线粒体DNA(mtDNA)中烟酰胺脱氢酶亚基Ⅳ基因(nad4)部分序列(pnad4),以期找出它们之间的序列差异和种群遗传关系。方法抽提61个弓首属蛔虫(包括犬弓首蛔虫、猫弓首蛔虫、马来西亚弓首蛔虫和犊弓首蛔虫)和狮弓蛔... 目的比较犬、猫常见蛔虫的线粒体DNA(mtDNA)中烟酰胺脱氢酶亚基Ⅳ基因(nad4)部分序列(pnad4),以期找出它们之间的序列差异和种群遗传关系。方法抽提61个弓首属蛔虫(包括犬弓首蛔虫、猫弓首蛔虫、马来西亚弓首蛔虫和犊弓首蛔虫)和狮弓蛔虫虫体的总DNA,用PCR方法扩增mtDNApnad4,然后用SSCP技术和DNA测序对序列进行分析研究。结果犬弓首蛔虫种群内pnad4的序列差异为1.17%,与猫弓首蛔虫、马来西亚弓首蛔虫、牛弓首蛔虫和狮弓蛔虫种间的平均序列差异分别为13.92%、16.48%、15.12%和20.32%。猫弓首蛔虫种群内pnad4的序列差异为1.00%,与马来西亚弓首蛔虫、牛弓首蛔虫、狮弓蛔虫平均序列差异分别是17.90%、13.55%和18.50%。马来西亚弓首蛔虫种群内pnad4序列差异为0.13%,与牛弓首蛔虫、狮弓蛔虫序列差异分别为13.50%和20.60%。结论犬弓首蛔虫不同地方虫株的pnad4有一定差异,猫弓首蛔虫种群内差异较小,马来西亚弓首蛔虫种群内差异很小。弓首属各虫种之间的差异以及与狮弓蛔虫的差异都较大。马来西亚弓首蛔虫pnad4序列与其它虫种差异都较大,证明它确为一独立种。pnad4序列是弓首蛔虫和狮弓蛔虫理想的种特异的遗传标记。 展开更多
关键词 犬弓首蛔虫 猫弓首蛔虫 马来西亚弓首蛔虫 牛弓首蛔虫 狮弓蛔虫 线粒体DNA 烟酰胺脱氢酶亚基Ⅳ基因 PCR-SSCP
下载PDF
A case report of ocular toxocariasis 被引量:1
2
作者 Azira NMS Zeehaida M 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2011年第2期164-165,共2页
Ocular toxocariasis is prevalent among children.The symptoms and signs may mimic other ocular pathologies such as malignancies and other infectious diseases(such as toxoplasmosis and syphilis). We presented a case of ... Ocular toxocariasis is prevalent among children.The symptoms and signs may mimic other ocular pathologies such as malignancies and other infectious diseases(such as toxoplasmosis and syphilis). We presented a case of progressive blurring of vision in a single eye of a 9-year-old boy.The presence of anti-toxocara antibody in serum samples helps to confirmation the diagnosis in our patient.Despite of treatment,the boy had lost his vision on the affected eye. 展开更多
关键词 OCULAR TOXOCARIASIS OCULAR larva MIGRANT toxocara cati VISION
下载PDF
东北虎源蛔虫线粒体cox1基因序列进化分析研究 被引量:3
3
作者 邱启官 谭磊 +1 位作者 商红帅 刘伟 《中国预防兽医学报》 CAS CSCD 北大核心 2017年第1期71-73,共3页
为了解东北虎源蛔虫与其他蛔虫的种群遗传关系,本研究对湖南省长沙生态动物园东北虎粪便中采集的蛔虫成虫的线粒体DNA中细胞色素I(cox1)基因序列进行扩增,将测序结果与GenB ank中登录的其他蛔虫cox1序列进行对比及种系发育树分析。结果... 为了解东北虎源蛔虫与其他蛔虫的种群遗传关系,本研究对湖南省长沙生态动物园东北虎粪便中采集的蛔虫成虫的线粒体DNA中细胞色素I(cox1)基因序列进行扩增,将测序结果与GenB ank中登录的其他蛔虫cox1序列进行对比及种系发育树分析。结果显示,来自长沙生态动物园7个东北虎蛔虫样品cox1序列长度基本一致,为413 bp^414 bp,与猫弓首蛔虫同源性最高,达98.4%以上,而且系统进化分析显示所有样品均与猫弓首蛔虫位于同一分支。该结果表明,pcox1序列种内相对保守,种间差异较大,因此可以作为猫科弓首蛔虫的种间遗传变异研究的分子遗传标记,从而为蛔虫的群体遗传研究奠定了基础。 展开更多
关键词 蛔虫 细胞色素I 序列分析 猫弓首蛔虫
下载PDF
东北虎圈养环境土壤中蛔虫虫卵的分离与鉴定 被引量:1
4
作者 张长生 彭智伟 +2 位作者 史沁欣 郝春晓 侯志军 《安徽农业科学》 CAS 2015年第11期127-128,131,共3页
[目的]对东北虎圈养环境土壤中蛔虫虫卵进行分离与鉴定。[方法]对黑龙江某虎园内的土壤进行随机采样,对蛔虫虫卵进行分离,通过形态学鉴定、分子生物学鉴定和分子进化树对其进行鉴定。[结果]通过形态学鉴定确定土壤中含有2种形态的虫卵... [目的]对东北虎圈养环境土壤中蛔虫虫卵进行分离与鉴定。[方法]对黑龙江某虎园内的土壤进行随机采样,对蛔虫虫卵进行分离,通过形态学鉴定、分子生物学鉴定和分子进化树对其进行鉴定。[结果]通过形态学鉴定确定土壤中含有2种形态的虫卵。分子生物学鉴定结果表明该虫卵分别是是狮弓蛔虫虫卵和猫弓首蛔虫虫卵。分子进化树分析结果表明狮弓蛔虫属于弓蛔属,猫弓首蛔虫属于弓首属。[结论]通过形态学观察、分子鉴定和进化树分析确定土壤中含有狮弓蛔虫虫卵和猫弓首蛔虫虫卵。 展开更多
关键词 东北虎 土壤 狮弓蛔虫 猫弓首蛔虫 分子生物学鉴定
下载PDF
Feral Cats: Parasitic Reservoirs in Our Zoos?
5
作者 Thalyta Ananias Lima Paula Andrea Borges Salgado +3 位作者 Carolina Romeiro Fernandes Chagas Patrícia Locosque Ramos Edson Aparecido Adriano Irys Hany Lima Gonzalez 《Open Journal of Veterinary Medicine》 2020年第8期126-138,共13页
<span style="line-height:1.5;">Up until the recent past, zoos served limited function, primarily existing for entertainment value. Today’s zoos, however, are serving many roles, chief among them: spec... <span style="line-height:1.5;">Up until the recent past, zoos served limited function, primarily existing for entertainment value. Today’s zoos, however, are serving many roles, chief among them: species conservation of captive animals. The biggest zoo in Brazil, S?o Paulo Zoological Park Foundation, has among its 2000 animals and many species of wild cats. The presence of domestic cats living freely in zoos </span><span style="line-height:1.5;">is</span><span style="line-height:1.5;"> common and can be a source of spreading disease. The aim of this study was to verify the variety and prevalence of parasites found in the feces of felids (feral and wild) living in the S<span style="white-space:nowrap;">?</span>o Paulo Zoo. The results of this parasitological analysis have been obtained from the laboratory of clinical analysis and correspond to the 4-year period beginning January/2009 and ending December/2012. Eight species of parasites were identified in the feces of captive wild cats and three in the feces of feral cats. For those captive</span><span style="line-height:1.5;">s</span><span "="" style="line-height:1.5;">, <i>Toxocara cati</i> (7.95%) had the highest prevalence, followed by <i>Toxascaris leonina</i> (7.58%), <i>Isospora</i> sp. (2.03%), <i>Hymenolepis nana</i> (0.92%), <i>Eimeria</i> sp., <i>Giardia</i> sp. and <i>Blastocystis</i> sp. (0.37% each) and <i>Ascaris</i> sp. (0.18%). Among the feral cats, we found <i>Toxocara cati</i> (59.26%), <i>Giardia</i> sp. (22.22%) and <i>Isospora</i> sp. (11.11%). For the captive group, we also distinguished natives from exotic species, finding native species to be more frequently parasitized than the exotic ones. Key to our findings, though, was the fact that a few parasite species were found among all groups of </span><span style="line-height:1.5;">f</span><span "="" style="line-height:1.5;">elids, specifically (<i>Toxocara cati</i>, <i>Giardia</i> sp. and <i>Isospora</i> sp). Further research is needed, however, to confirm that transmission of these parasite 展开更多
关键词 Enteroparasites FELIDS Felis catus toxocara cati Wild Animals
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部