目的:研究龙血竭凝胶剂的制备工艺,并评价其创面组织修复作用。方法:采用正交试验,以卡波姆、丙二醇、p H值为考察因素,以凝胶剂的外观、刺激性、酸碱度、耐热、耐寒和加速离心实验为考查指标,筛选凝胶剂最佳基质处方;小鼠创面组织修复...目的:研究龙血竭凝胶剂的制备工艺,并评价其创面组织修复作用。方法:采用正交试验,以卡波姆、丙二醇、p H值为考察因素,以凝胶剂的外观、刺激性、酸碱度、耐热、耐寒和加速离心实验为考查指标,筛选凝胶剂最佳基质处方;小鼠创面组织修复实验,30只昆明种小鼠,随机分为3组:模型组、龙血竭凝胶剂组、克林霉素利多卡因凝胶剂组,在小鼠背部切取直径为1.2 cm的创伤动物模型,以龙血竭凝胶剂为实验组,克林霉素利多卡因凝胶剂为阳性组,凝胶剂基质为模型组处理创伤动物模型,在不同时间点观察创面愈合情况,并对创面病理切片进行组织病理学评分。结果:优选处方为卡波姆-940 0.5%,丙二醇2.5 m L,p H 8.0。小鼠创伤模型涂抹克林霉素利多卡因凝胶剂和龙血竭凝胶剂,创面无感染,愈合面光滑平整;比较创伤修复结果,龙血竭凝胶剂与克林霉素利多卡因凝胶剂较模型组创面愈合时间显著缩短,具有显著性差异(P<0.05)。结论:该凝胶剂处方和制备工艺稳定可行,凝胶剂外观颜色均匀,涂布性能良好,耐寒耐热试验均无变化,龙血竭凝胶剂对小鼠创伤模型具有明显组织修复作用。展开更多
The Ca 2+-binding protein of the EF-hand type,S100B,exerts both intracellular and extracellular regulatory activities.As an intracellular regulator,S100B is involved in the regulation of energy metabolism,transcriptio...The Ca 2+-binding protein of the EF-hand type,S100B,exerts both intracellular and extracellular regulatory activities.As an intracellular regulator,S100B is involved in the regulation of energy metabolism,transcription,protein phosphorylation,cell proliferation,survival,differentiation and motility,and Ca 2+ homeostasis,by interacting with a wide array of proteins(i.e.,enzymes,enzyme substrates,cytoskeletal subunits,scaffold/adaptor proteins,transcription factors,ubiquitin E3 ligases,ion channels) in a restricted number of cell types.As an extracellular signal,S100B engages the pattern recognition receptor,receptor for advanced glycation end-products(RAGE),on immune cells as well as on neuronal,astrocytic and microglial cells,vascular smooth muscle cells,skeletal myoblasts and cardiomyocytes.However,RAGE may not be the sole receptor activated by S100B,the protein being able to enhance bFGF-FGFR1 signaling by interacting with FGFR1-bound bFGF in particular cell types.Moreover,extracellular effects of S100B vary depending on its local concentration.Increasing evidence suggests that at the concentration found in extracellular fluids in normal physiological conditions and locally upon acute tissue injury,which is up to a few nM levels,S100B exerts trophic effects in the central and peripheral nervous system and in skeletal muscle tissue thus participating in tissue homeostasis.The present commentary summarizes results implicating intracellular and extracellular S100B in tissue development,repair and regeneration.展开更多
文摘目的:研究龙血竭凝胶剂的制备工艺,并评价其创面组织修复作用。方法:采用正交试验,以卡波姆、丙二醇、p H值为考察因素,以凝胶剂的外观、刺激性、酸碱度、耐热、耐寒和加速离心实验为考查指标,筛选凝胶剂最佳基质处方;小鼠创面组织修复实验,30只昆明种小鼠,随机分为3组:模型组、龙血竭凝胶剂组、克林霉素利多卡因凝胶剂组,在小鼠背部切取直径为1.2 cm的创伤动物模型,以龙血竭凝胶剂为实验组,克林霉素利多卡因凝胶剂为阳性组,凝胶剂基质为模型组处理创伤动物模型,在不同时间点观察创面愈合情况,并对创面病理切片进行组织病理学评分。结果:优选处方为卡波姆-940 0.5%,丙二醇2.5 m L,p H 8.0。小鼠创伤模型涂抹克林霉素利多卡因凝胶剂和龙血竭凝胶剂,创面无感染,愈合面光滑平整;比较创伤修复结果,龙血竭凝胶剂与克林霉素利多卡因凝胶剂较模型组创面愈合时间显著缩短,具有显著性差异(P<0.05)。结论:该凝胶剂处方和制备工艺稳定可行,凝胶剂外观颜色均匀,涂布性能良好,耐寒耐热试验均无变化,龙血竭凝胶剂对小鼠创伤模型具有明显组织修复作用。
基金Supported by Ministero dell'Università e della Ricerca,No. PRIN 2007LNKSYS,No.2007AWZTHH_004 and No.2009WBFZYM_002Association Fran aise contre les Myopathies,No. Project 12992+1 种基金Associazione Italiana per la Ricerca sul Cancro,No. Project 6021Fondazione Cassa di Risparmio di Perugia,No. 2007.0218.020,No.2009.020.0021 and No.2012.0241.021
文摘The Ca 2+-binding protein of the EF-hand type,S100B,exerts both intracellular and extracellular regulatory activities.As an intracellular regulator,S100B is involved in the regulation of energy metabolism,transcription,protein phosphorylation,cell proliferation,survival,differentiation and motility,and Ca 2+ homeostasis,by interacting with a wide array of proteins(i.e.,enzymes,enzyme substrates,cytoskeletal subunits,scaffold/adaptor proteins,transcription factors,ubiquitin E3 ligases,ion channels) in a restricted number of cell types.As an extracellular signal,S100B engages the pattern recognition receptor,receptor for advanced glycation end-products(RAGE),on immune cells as well as on neuronal,astrocytic and microglial cells,vascular smooth muscle cells,skeletal myoblasts and cardiomyocytes.However,RAGE may not be the sole receptor activated by S100B,the protein being able to enhance bFGF-FGFR1 signaling by interacting with FGFR1-bound bFGF in particular cell types.Moreover,extracellular effects of S100B vary depending on its local concentration.Increasing evidence suggests that at the concentration found in extracellular fluids in normal physiological conditions and locally upon acute tissue injury,which is up to a few nM levels,S100B exerts trophic effects in the central and peripheral nervous system and in skeletal muscle tissue thus participating in tissue homeostasis.The present commentary summarizes results implicating intracellular and extracellular S100B in tissue development,repair and regeneration.
