Tibetan medicinal plants have been used for more than 2 000 years. In order to find their differences in antioxidant activity, total phenolics and total flavonoids between "hot-nature" and "cold-nature&...Tibetan medicinal plants have been used for more than 2 000 years. In order to find their differences in antioxidant activity, total phenolics and total flavonoids between "hot-nature" and "cold-nature" herbs, we investigated the antioxidant activities of 40 Tibetan herbs from Qinghai plateau, with 20 herbs in cold-nature and 20 herbs in hot-nature. Antioxidant capacities were evaluated by the following methods: scavenging ABTS?+(2, 2′azinobis-(3-ethylbenz-thiazoline-6-sulfonic acid), scavenging O2?-, and Ferric-reducing antioxidant power(FRAP). The effects on inhibition of mitochondrion lipid peroxidation were determined by measuring the formation of TBARS(Thiobarbituric acid reactive substrates). Total phenolics and flavonoids were estimated by Folin-Ciocalteu and Na NO2-Al(NO3)3-Na OH colorimetric methods. Interestingly, the cold-nature herbs displayed higher antioxidant activities than the hot-nature ones, corresponding to nearly three-fold higher total phenolic contents in the cold-nature herbs. Moreover, the antioxidant activities correlated linearly with the levels of total phenolics for both cold-nature and hot-nature herbs, but only with the levels of total flavonoids for the hot-nature herbs. The results suggested that the phenolic compounds, but not the flavonoids, play the major role in antioxidant capacities of the cold-nature herbs. These findings could shed new lights on the study the theory of Tibetan medicine.展开更多
目的:建立HPLC同时测定旺拉中4种促智类有效成分的含量测定方法,并对不同产地的旺拉进行含量测定,以建立旺拉的质量控制标准。方法:采用C18反相色谱柱,甲醇-水梯度洗脱,0 min 15%~10 min 20%~20 min 45%~35 min70%,流速0.7mL.min-1,在22...目的:建立HPLC同时测定旺拉中4种促智类有效成分的含量测定方法,并对不同产地的旺拉进行含量测定,以建立旺拉的质量控制标准。方法:采用C18反相色谱柱,甲醇-水梯度洗脱,0 min 15%~10 min 20%~20 min 45%~35 min70%,流速0.7mL.min-1,在222 nm波长处检测dactylorhin B,loroglossin,dactylorhin A,militarine的含量。正交实验优化药材提取方法并测定24个不同产地样品的含量。结果:建立了藏药旺拉HPLC分析方法,dactylorhin B,loroglossin,dactylorhin A,militarine在测定的范围内均表现出良好的线性(r=0.999 9);方法的回收率在99.00%~99.52%,RSD<1.0%。结论:高效液相色谱法,能简便快速的测定旺拉中有效成分的含量,鉴别不同产地药材质量优劣,为全面控制藏药旺拉提供了依据。展开更多
基金supported by Gansu Provincial Natural Science Foundation(No.1208RJYA014)Chinese Postdoctoral Science Foundation(No.2009-0460744)+1 种基金Fundamental Research Funds for the Central Universities(No.lzujbky-2014-151)the Natural Science Foundation of China(No.21302079)
文摘Tibetan medicinal plants have been used for more than 2 000 years. In order to find their differences in antioxidant activity, total phenolics and total flavonoids between "hot-nature" and "cold-nature" herbs, we investigated the antioxidant activities of 40 Tibetan herbs from Qinghai plateau, with 20 herbs in cold-nature and 20 herbs in hot-nature. Antioxidant capacities were evaluated by the following methods: scavenging ABTS?+(2, 2′azinobis-(3-ethylbenz-thiazoline-6-sulfonic acid), scavenging O2?-, and Ferric-reducing antioxidant power(FRAP). The effects on inhibition of mitochondrion lipid peroxidation were determined by measuring the formation of TBARS(Thiobarbituric acid reactive substrates). Total phenolics and flavonoids were estimated by Folin-Ciocalteu and Na NO2-Al(NO3)3-Na OH colorimetric methods. Interestingly, the cold-nature herbs displayed higher antioxidant activities than the hot-nature ones, corresponding to nearly three-fold higher total phenolic contents in the cold-nature herbs. Moreover, the antioxidant activities correlated linearly with the levels of total phenolics for both cold-nature and hot-nature herbs, but only with the levels of total flavonoids for the hot-nature herbs. The results suggested that the phenolic compounds, but not the flavonoids, play the major role in antioxidant capacities of the cold-nature herbs. These findings could shed new lights on the study the theory of Tibetan medicine.
文摘目的:建立HPLC同时测定旺拉中4种促智类有效成分的含量测定方法,并对不同产地的旺拉进行含量测定,以建立旺拉的质量控制标准。方法:采用C18反相色谱柱,甲醇-水梯度洗脱,0 min 15%~10 min 20%~20 min 45%~35 min70%,流速0.7mL.min-1,在222 nm波长处检测dactylorhin B,loroglossin,dactylorhin A,militarine的含量。正交实验优化药材提取方法并测定24个不同产地样品的含量。结果:建立了藏药旺拉HPLC分析方法,dactylorhin B,loroglossin,dactylorhin A,militarine在测定的范围内均表现出良好的线性(r=0.999 9);方法的回收率在99.00%~99.52%,RSD<1.0%。结论:高效液相色谱法,能简便快速的测定旺拉中有效成分的含量,鉴别不同产地药材质量优劣,为全面控制藏药旺拉提供了依据。
