中国明对虾(Fenneropenaeus chinensis)的性别分化和生殖器官发育是其繁殖生物学的重要部分,也是进行性别调控的基础。经过2年的取样观察,研究了中国明对虾雌性纳精囊和雄性交接器的分化与发育过程。结果显示,早在仔虾后16 d(16 days po...中国明对虾(Fenneropenaeus chinensis)的性别分化和生殖器官发育是其繁殖生物学的重要部分,也是进行性别调控的基础。经过2年的取样观察,研究了中国明对虾雌性纳精囊和雄性交接器的分化与发育过程。结果显示,早在仔虾后16 d(16 days post-larva,PL16)即开始雌雄分化,此时,雌虾第4与第5对步足间腹甲处的锥突出现明显下陷,而雄虾的没有下陷。PL54时,雌虾纳精囊瓣膜出现,然后继续发育,到PL124时形成纳精囊雏形;雄虾交接器发育较晚,到PL54时,雄虾第1泳足的内肢才出现分化,在PL106基本形成雄性交接器。展开更多
The crucial step of most of the current genetic studies is the extraction of DNA of sufficient quantity and quality.Several genomic DNA isolation methods have been described to successfully obtain male DNA from shrimp...The crucial step of most of the current genetic studies is the extraction of DNA of sufficient quantity and quality.Several genomic DNA isolation methods have been described to successfully obtain male DNA from shrimp species.However,all current protocols require invasive handling methods with males for DNA isolation.Using Aristeus antennatus as a model we tested a reliable non-invasive differential DNA extraction method to male DNA isolation from spermatophores attached to female thelycum.The present protocol provides high quality and quantity DNA for polymerase chain reaction amplification and male genotyping.This new approach could be useful to experimental shrimp culture to select sires with relevant genetic patterns for selective breeding programs.More importantly,it can be applied to identify the mating pairs and male structure in wild populations of species as A.antennatus,where males are often difficult to capture.Our method could be also valuable for biological studies on other spermatophore-using species,such as myriapods,arachnids and insects.展开更多
文摘中国明对虾(Fenneropenaeus chinensis)的性别分化和生殖器官发育是其繁殖生物学的重要部分,也是进行性别调控的基础。经过2年的取样观察,研究了中国明对虾雌性纳精囊和雄性交接器的分化与发育过程。结果显示,早在仔虾后16 d(16 days post-larva,PL16)即开始雌雄分化,此时,雌虾第4与第5对步足间腹甲处的锥突出现明显下陷,而雄虾的没有下陷。PL54时,雌虾纳精囊瓣膜出现,然后继续发育,到PL124时形成纳精囊雏形;雄虾交接器发育较晚,到PL54时,雄虾第1泳足的内肢才出现分化,在PL106基本形成雄性交接器。
基金supported by a grant from Spanish Ministerio de Economía y Competitividad(CTM2014-54648-C2-2-R)MIR.LP benefited from predoctoral fellowship from the Universitat de Girona(BR2014).
文摘The crucial step of most of the current genetic studies is the extraction of DNA of sufficient quantity and quality.Several genomic DNA isolation methods have been described to successfully obtain male DNA from shrimp species.However,all current protocols require invasive handling methods with males for DNA isolation.Using Aristeus antennatus as a model we tested a reliable non-invasive differential DNA extraction method to male DNA isolation from spermatophores attached to female thelycum.The present protocol provides high quality and quantity DNA for polymerase chain reaction amplification and male genotyping.This new approach could be useful to experimental shrimp culture to select sires with relevant genetic patterns for selective breeding programs.More importantly,it can be applied to identify the mating pairs and male structure in wild populations of species as A.antennatus,where males are often difficult to capture.Our method could be also valuable for biological studies on other spermatophore-using species,such as myriapods,arachnids and insects.
