The production of elastase by Bacillus sp. EL31410 at various temperatures was investigated. In order to study the effect of temperature on elastase fermentation, different cultivation temperatures, ranging from 39 ℃...The production of elastase by Bacillus sp. EL31410 at various temperatures was investigated. In order to study the effect of temperature on elastase fermentation, different cultivation temperatures, ranging from 39 ℃ to 28 ℃, were evaluated in shake flask. The result indicated that 37 ℃ was best for cell growth at earlier stage; while maximum elastase activity was obtained when the cells were cultivated at 30 ℃. This result was verified by batch fermentation in 5-L bio-reactor under 37 ℃ and 30 ℃ temperature, respectively. The specific cell growth rate at 37 ℃ was higher than that at 30 ℃ during earlier stage of cultivation. The maximum value [5.5 U/(h-g DCW)] of elastase formation rate occurred at 24 h at 30 ℃ compared to 4.6 U/(h-g DCW) at 30 h at 37 ℃. Based on these results, two-stage temperature shift strategy and oscillatory temperature cultivation mode were evaluated in the next study. When compared to single temperature of 37 ℃ or 30 ℃, both two-stage temperature shift strategy and oscillatory temperature strategy improved biomass but did not yield the same result as expected for elastase production. The maximum biomass (both 8.6 g/L) was achieved at 30 h at 37 ℃, but at 42 h using two-stage temperature cultivation strategy. The highest elastase production (652 U/ml) was observed at 30 ℃ in batch process. It was concluded that cultivation at constant temperature of 30 ℃ was appropriate for elastase production by Bacillus sp. EL31410.展开更多
A two-stage process with temperature-shift has been developed to enhance the anthocyanin yield in suspension cultures of strawbeny cells. The effect of the temperature-shift interval and the shift-time point was inves...A two-stage process with temperature-shift has been developed to enhance the anthocyanin yield in suspension cultures of strawbeny cells. The effect of the temperature-shift interval and the shift-time point was investigated for the optimization of this strategy. In this process, strawbeny cells were grown at 30°C (the optimum temperature for cell gowth) for a certain period as the first stage, with the temperature shifted to a lower temperature for the second stage. In response to the temperature shift-down, anthocyanin synthesis was stimulated and a higher content could be achieved than that at both boundary temperatures but cell growth was suppressed. When the lower boundary temperature. was decreased, cell growth was lowered and a delayed, sustained maximum anthocyanin content was achieved. Anthocyanin synthesis was strongly influenced by the shift-time point but cell growth was not. Consequently, the maximum anthocyanin content of 2.7 mg.g-fresh cell-1 was obtained on day 9 by a temperature-shift from 309°C, after 3-d culture, to 15°C. The highest anthocyanin yield of 318 mg. L-1 on day 12 was achieved when the temperature was shifted from 30°C, after 5-d culture, to 20°C. For a global optimization of both the yield and productivity, the optimum anthocyanin yield and productivity of 272 mg.L-1 1 and 30.2 mg.L-1d1 on day 9 were achieved by a two-stage culture with a temperature-shift from 30°C after 3 d to 20°C.展开更多
Alterations of annual temperature cycles have profound implications on how the planet responds to global climate change. In this study, a high resolution global analysis of temperature cycle shifts and their developme...Alterations of annual temperature cycles have profound implications on how the planet responds to global climate change. In this study, a high resolution global analysis of temperature cycle shifts and their development over time is presented. We show that over the last 63 years, phase shifts in the annual near surface temperature cycle exhibit large spatiotemporal variability. The calculated phase shifts comprise earlier onsets of seasons as well as delays with similar frequencies, depending on location. From 1978 to 2010 Eastern Europe experienced an advanced annual cycle of near-surface temperature of 3.2 days while Eastern Australia shows an opposite shift towards later seasons of 3.5 days in comparison to the preceding 30-year period from 1948 to 1977. The largest phase shifts of –5.5 days toward earlier seasons over land were found in Belarus and Northwest Russia. For the first time the developments of seasonal temperature shifts were generalized for large areas by using self-organizing feature map neural networks resulting into 4 significant global trends. The temperature phase shifts are also shown to have strong correlations with the timing of shrub foliation observed at 57 phenological stations across the USA. The findings have far-reaching, yet regionally distinct consequences on agriculture, animal life cycles, plant phenology, and regional weather phenomena that change with annual temperature cycles.展开更多
The change of sea surface temperature（SST） in the southern Indian Ocean（SIO） during the recent six decades has been analyzed based on oceanic reanalysis and model, as well as atmospheric data. The results show tha...The change of sea surface temperature（SST） in the southern Indian Ocean（SIO） during the recent six decades has been analyzed based on oceanic reanalysis and model, as well as atmospheric data. The results show that a thermal regime shift in SIO during the 1960 s, which is not caught enough attentions, has been of equal magnitude to the linear warming since 1970. Empirical Orthogonal Function（EOF） analyses reveal that a thermal shift is combined with atmospheric changes such as the weakening of westerly during the period of 1960–1967. Inner dynamic connections can be defined that when the westerly winds turn weak, the anticyclonic wind circulation between westerly winds and the trade winds decreases, which further reduces the SST to a negative peak in this period. It is noted that the shifts in the 1960 s are also evident for Southern Hemisphere. For example, subtropical high and the entire westerly winds belt at high latitudes both change dramatically in the 1960 s. This large-scaled process maybe link to the change of southern annular mode（SAM）.展开更多
文摘The production of elastase by Bacillus sp. EL31410 at various temperatures was investigated. In order to study the effect of temperature on elastase fermentation, different cultivation temperatures, ranging from 39 ℃ to 28 ℃, were evaluated in shake flask. The result indicated that 37 ℃ was best for cell growth at earlier stage; while maximum elastase activity was obtained when the cells were cultivated at 30 ℃. This result was verified by batch fermentation in 5-L bio-reactor under 37 ℃ and 30 ℃ temperature, respectively. The specific cell growth rate at 37 ℃ was higher than that at 30 ℃ during earlier stage of cultivation. The maximum value [5.5 U/(h-g DCW)] of elastase formation rate occurred at 24 h at 30 ℃ compared to 4.6 U/(h-g DCW) at 30 h at 37 ℃. Based on these results, two-stage temperature shift strategy and oscillatory temperature cultivation mode were evaluated in the next study. When compared to single temperature of 37 ℃ or 30 ℃, both two-stage temperature shift strategy and oscillatory temperature strategy improved biomass but did not yield the same result as expected for elastase production. The maximum biomass (both 8.6 g/L) was achieved at 30 h at 37 ℃, but at 42 h using two-stage temperature cultivation strategy. The highest elastase production (652 U/ml) was observed at 30 ℃ in batch process. It was concluded that cultivation at constant temperature of 30 ℃ was appropriate for elastase production by Bacillus sp. EL31410.
文摘A two-stage process with temperature-shift has been developed to enhance the anthocyanin yield in suspension cultures of strawbeny cells. The effect of the temperature-shift interval and the shift-time point was investigated for the optimization of this strategy. In this process, strawbeny cells were grown at 30°C (the optimum temperature for cell gowth) for a certain period as the first stage, with the temperature shifted to a lower temperature for the second stage. In response to the temperature shift-down, anthocyanin synthesis was stimulated and a higher content could be achieved than that at both boundary temperatures but cell growth was suppressed. When the lower boundary temperature. was decreased, cell growth was lowered and a delayed, sustained maximum anthocyanin content was achieved. Anthocyanin synthesis was strongly influenced by the shift-time point but cell growth was not. Consequently, the maximum anthocyanin content of 2.7 mg.g-fresh cell-1 was obtained on day 9 by a temperature-shift from 309°C, after 3-d culture, to 15°C. The highest anthocyanin yield of 318 mg. L-1 on day 12 was achieved when the temperature was shifted from 30°C, after 5-d culture, to 20°C. For a global optimization of both the yield and productivity, the optimum anthocyanin yield and productivity of 272 mg.L-1 1 and 30.2 mg.L-1d1 on day 9 were achieved by a two-stage culture with a temperature-shift from 30°C after 3 d to 20°C.
文摘Alterations of annual temperature cycles have profound implications on how the planet responds to global climate change. In this study, a high resolution global analysis of temperature cycle shifts and their development over time is presented. We show that over the last 63 years, phase shifts in the annual near surface temperature cycle exhibit large spatiotemporal variability. The calculated phase shifts comprise earlier onsets of seasons as well as delays with similar frequencies, depending on location. From 1978 to 2010 Eastern Europe experienced an advanced annual cycle of near-surface temperature of 3.2 days while Eastern Australia shows an opposite shift towards later seasons of 3.5 days in comparison to the preceding 30-year period from 1948 to 1977. The largest phase shifts of –5.5 days toward earlier seasons over land were found in Belarus and Northwest Russia. For the first time the developments of seasonal temperature shifts were generalized for large areas by using self-organizing feature map neural networks resulting into 4 significant global trends. The temperature phase shifts are also shown to have strong correlations with the timing of shrub foliation observed at 57 phenological stations across the USA. The findings have far-reaching, yet regionally distinct consequences on agriculture, animal life cycles, plant phenology, and regional weather phenomena that change with annual temperature cycles.
基金The National Natural Science Foundation of China for Distinguished Young Scholars and Innovative Research Groups under contract Nos 41525019 and 41521005the Project of the State Oceanic Administration of China for Global Climate Change under contract No.GASI-IPOVAI-02the CAS/SAFEA International Partnership Program for Creative Research Teams
文摘The change of sea surface temperature（SST） in the southern Indian Ocean（SIO） during the recent six decades has been analyzed based on oceanic reanalysis and model, as well as atmospheric data. The results show that a thermal regime shift in SIO during the 1960 s, which is not caught enough attentions, has been of equal magnitude to the linear warming since 1970. Empirical Orthogonal Function（EOF） analyses reveal that a thermal shift is combined with atmospheric changes such as the weakening of westerly during the period of 1960–1967. Inner dynamic connections can be defined that when the westerly winds turn weak, the anticyclonic wind circulation between westerly winds and the trade winds decreases, which further reduces the SST to a negative peak in this period. It is noted that the shifts in the 1960 s are also evident for Southern Hemisphere. For example, subtropical high and the entire westerly winds belt at high latitudes both change dramatically in the 1960 s. This large-scaled process maybe link to the change of southern annular mode（SAM）.
