以苹果(平邑甜茶)幼苗为试验材料,盆栽条件下以万寿菊植株风干粉末拌土熏蒸老苹果园土壤,为环境友好型熏蒸措施防控苹果连作障碍提供理论依据。试验设置老龄苹果园土壤对照(未作处理,CK)、覆膜(未拌入万寿菊,F)、万寿菊1.5 g kg^(-1)+覆...以苹果(平邑甜茶)幼苗为试验材料,盆栽条件下以万寿菊植株风干粉末拌土熏蒸老苹果园土壤,为环境友好型熏蒸措施防控苹果连作障碍提供理论依据。试验设置老龄苹果园土壤对照(未作处理,CK)、覆膜(未拌入万寿菊,F)、万寿菊1.5 g kg^(-1)+覆膜(1.5T+F)、万寿菊6.0 g kg^(-1)+覆膜(6.0T+F)、万寿菊15.0 g kg^(-1)+覆膜(15.0T+F)。结果表明,与对照相比,万寿菊生物熏蒸处理能显著提高连作土壤中平邑甜茶幼苗的生物量,促进根系生长,提高土壤酶活性,土壤真菌减少、细菌增多。6.0 g kg^(-1)处理的效果最明显,幼苗地上部及根系干重分别为对照的8.1倍、13.1倍,根表面积及根体积分别增加了333.0%、548.4%;脲酶、磷酸酶活性分别较对照高103.6%、77.6%,蔗糖酶活性高出200.4%,过氧化氢酶活性升高64.6%;土壤细菌/真菌比值为219.9,是对照的5.6倍;真菌多样性、均匀度和丰富度指数均降低,优势度指数增加;层出镰孢菌基因拷贝数较对照降低57.9%。主成分分析(PCA)结果显示,万寿菊生物熏蒸处理对连作土壤真菌群落多样性有显著影响,能够显著减少层出镰孢菌的数量。综上,连作土壤中添加6.0 g kg^(-1)万寿菊进行生物熏蒸可提高连作平邑甜茶幼苗生物量,改善连作土壤环境,有效缓解平邑甜茶的连作障碍。展开更多
The toxicities of extracts of Tateges erecta against Tetranychus viennensis, and the effects on several enzymes of Tetranychus viennensis conducted with the chloroform extracts were studied. The results showed that th...The toxicities of extracts of Tateges erecta against Tetranychus viennensis, and the effects on several enzymes of Tetranychus viennensis conducted with the chloroform extracts were studied. The results showed that the extracts of Tateges erecta possessed high bioactivity against Tetranychus viennensis. In photic group, when treated with the extracts of chloroform, petroleum ether and methanol, the LC_ 50 were 5.833, 3.508 and 8.445 9 mg·mL -1 respectively. The activities of glutathiona {S_transferase} and esterase isozyme can be strongly inhibited by the chloroform extracts in the photic group. In dark group, the activity of glutathiona S_transferase and protease were induced and inhibited respectively, the variety of esterase isozyme was not obvious.展开更多
The activities of superoxide dismutase （SOD） ,peroxidase（POD） and catalase（CAT） in 3th instar Chihuo zao were tested with conventional biochemical method after photo and dark reactivation treated by the chlorofo...The activities of superoxide dismutase （SOD） ,peroxidase（POD） and catalase（CAT） in 3th instar Chihuo zao were tested with conventional biochemical method after photo and dark reactivation treated by the chloroform extracts（TPC）which was obtained by sohxlet method from the root of Tateges erecta. The results showed :In the dark,the activities of POD,and CAT in treated group were obviously significant higher than those in contrasting group;In the light,the activities of SOD,POD,and CAT could be inhibited by TPC,after larvae had been exposed to photo lights radiation for 1 h. The more longer of the photo lights radiation,the more stronger of the inhibition. The results indicated that TPC possessed high photo-activity.展开更多
文摘以苹果(平邑甜茶)幼苗为试验材料,盆栽条件下以万寿菊植株风干粉末拌土熏蒸老苹果园土壤,为环境友好型熏蒸措施防控苹果连作障碍提供理论依据。试验设置老龄苹果园土壤对照(未作处理,CK)、覆膜(未拌入万寿菊,F)、万寿菊1.5 g kg^(-1)+覆膜(1.5T+F)、万寿菊6.0 g kg^(-1)+覆膜(6.0T+F)、万寿菊15.0 g kg^(-1)+覆膜(15.0T+F)。结果表明,与对照相比,万寿菊生物熏蒸处理能显著提高连作土壤中平邑甜茶幼苗的生物量,促进根系生长,提高土壤酶活性,土壤真菌减少、细菌增多。6.0 g kg^(-1)处理的效果最明显,幼苗地上部及根系干重分别为对照的8.1倍、13.1倍,根表面积及根体积分别增加了333.0%、548.4%;脲酶、磷酸酶活性分别较对照高103.6%、77.6%,蔗糖酶活性高出200.4%,过氧化氢酶活性升高64.6%;土壤细菌/真菌比值为219.9,是对照的5.6倍;真菌多样性、均匀度和丰富度指数均降低,优势度指数增加;层出镰孢菌基因拷贝数较对照降低57.9%。主成分分析(PCA)结果显示,万寿菊生物熏蒸处理对连作土壤真菌群落多样性有显著影响,能够显著减少层出镰孢菌的数量。综上,连作土壤中添加6.0 g kg^(-1)万寿菊进行生物熏蒸可提高连作平邑甜茶幼苗生物量,改善连作土壤环境,有效缓解平邑甜茶的连作障碍。
文摘The toxicities of extracts of Tateges erecta against Tetranychus viennensis, and the effects on several enzymes of Tetranychus viennensis conducted with the chloroform extracts were studied. The results showed that the extracts of Tateges erecta possessed high bioactivity against Tetranychus viennensis. In photic group, when treated with the extracts of chloroform, petroleum ether and methanol, the LC_ 50 were 5.833, 3.508 and 8.445 9 mg·mL -1 respectively. The activities of glutathiona {S_transferase} and esterase isozyme can be strongly inhibited by the chloroform extracts in the photic group. In dark group, the activity of glutathiona S_transferase and protease were induced and inhibited respectively, the variety of esterase isozyme was not obvious.
文摘The activities of superoxide dismutase （SOD） ,peroxidase（POD） and catalase（CAT） in 3th instar Chihuo zao were tested with conventional biochemical method after photo and dark reactivation treated by the chloroform extracts（TPC）which was obtained by sohxlet method from the root of Tateges erecta. The results showed :In the dark,the activities of POD,and CAT in treated group were obviously significant higher than those in contrasting group;In the light,the activities of SOD,POD,and CAT could be inhibited by TPC,after larvae had been exposed to photo lights radiation for 1 h. The more longer of the photo lights radiation,the more stronger of the inhibition. The results indicated that TPC possessed high photo-activity.
