Objective:To investigate the differential gene expression profiles in coronary heart disease(CHD) patients of blood-stasis syndrome(BSS) by oligonucleotide microarray technique,and the clinical significance of target ...Objective:To investigate the differential gene expression profiles in coronary heart disease(CHD) patients of blood-stasis syndrome(BSS) by oligonucleotide microarray technique,and the clinical significance of target gene.Methods:Subjects were assigned to CHD patients with BSS(n=8),CHD patients without BSS (n=8),and BSS patients without CHD(n=8) based on coronary angiography and the diagnostic criteria of BSS. The sex- and age-matched healthy volunteers(n=8) were enrolled as the control group.Venous blood s...展开更多
目的:基于网络药理学,探讨"金银花-板蓝根"药对的功效物质基础和配伍机制。方法:通过数据库检索获得金银花和板蓝根的化学成分、作用靶点及相关疾病等信息,构建"药物-成分-靶标-疾病"网络,进行基因本体(gene ontolo...目的:基于网络药理学,探讨"金银花-板蓝根"药对的功效物质基础和配伍机制。方法:通过数据库检索获得金银花和板蓝根的化学成分、作用靶点及相关疾病等信息,构建"药物-成分-靶标-疾病"网络,进行基因本体(gene ontology,GO)分类富集分析、基于京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)的通路富集分析,研究"金银花-板蓝根"药对的作用机制。结果:"药物-成分-靶标-疾病"网络包含2个药物,92种活性成分,相应靶标665个,相关疾病609种。关键靶点涉及前列腺素内过氧化物合酶2等,关键疾病涉及多种恶性肿瘤和炎症等。GO富集分析包含GO条目1 680个,其中生物过程相关条目1 367个,分子功能相关条目235个,细胞组分相关条目78个。KEGG通路富集分析包含KEGG通路137条,主要涉及多种恶性肿瘤信号通路和多种心血管疾病相关的信号通路。结论:本研究结果初步验证了"金银花-板蓝根"药对的基本药理作用及其机制,并为进一步深入揭示其作用机制奠定了良好基础。展开更多
The miRNA expression profile was initially established to investigate its corresponding function in human uveal melanoma. The miRNA expression profile in human uveal melanoma was analyzed by a micro chip technique.The...The miRNA expression profile was initially established to investigate its corresponding function in human uveal melanoma. The miRNA expression profile in human uveal melanoma was analyzed by a micro chip technique.The hsa-miRNA expression between four uveal melanomas and four normal uveal tissues was compared.Based on the bioinformatic approach,chip data was analyzed to select out differentially expressed candidate hsa-miRNAs.Real-time quantitative PCR(RT-PCR) was used to confirm the candidate hsa-miRNAs expression in all samples.The results of miRNA microarray chips that matched with RT-PCR were considered as the miRNA expression which was significantly different between normal tissue and uveal melanomas.In four uveal melanomas,expressions of miRNA-20a,miRNA-106a,miRNA-17,miRNA-21,and miRNA-34a were significantly up-regulated,while miRNA-145 and miRNA-204 expression were significantly down-regulated.We used miRNA microarray analysis as a fast,efficient technology to study biological information.The differentially expressed miRNAs may be involved in uveal melanoma pathogenesis,and may help promote the diagnosis and treatment for uveal melanoma.展开更多
Recently, the identification of miRNA targets has received much attention. The strategies to determine miRNA targets include bioinformatic prediction and experimental assays. The bioinformatic prediction methods are m...Recently, the identification of miRNA targets has received much attention. The strategies to determine miRNA targets include bioinformatic prediction and experimental assays. The bioinformatic prediction methods are mainly based on the confirmed rules of interaction between miRNAs and their targets, and are carried out by programs, such as miRanda, TargetScan, TargetScanS, RNAhybrid, DIANA-microT, PicTar, RNA22 and FindTar, which follow well-known principles. The experimental assays to find miRNA targets employ immunoprecipitation of AGO proteins to identify interacting mRNAs, or the analysis of mRNA or protein levels to identify genes which can be regulated by miRNAs. The improvement of current bioinformatic and experimental assays and the development of novel assays will enable greater efficiency in the identification of miRNA targets and thus facilitate miRNA research. This paper describes progress in the prediction and identification of miRNA targets.展开更多
基金Supported by the Major Research Plan of National Natural Science Foundation of China(No.90409021)
文摘Objective:To investigate the differential gene expression profiles in coronary heart disease(CHD) patients of blood-stasis syndrome(BSS) by oligonucleotide microarray technique,and the clinical significance of target gene.Methods:Subjects were assigned to CHD patients with BSS(n=8),CHD patients without BSS (n=8),and BSS patients without CHD(n=8) based on coronary angiography and the diagnostic criteria of BSS. The sex- and age-matched healthy volunteers(n=8) were enrolled as the control group.Venous blood s...
文摘目的:基于网络药理学,探讨"金银花-板蓝根"药对的功效物质基础和配伍机制。方法:通过数据库检索获得金银花和板蓝根的化学成分、作用靶点及相关疾病等信息,构建"药物-成分-靶标-疾病"网络,进行基因本体(gene ontology,GO)分类富集分析、基于京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)的通路富集分析,研究"金银花-板蓝根"药对的作用机制。结果:"药物-成分-靶标-疾病"网络包含2个药物,92种活性成分,相应靶标665个,相关疾病609种。关键靶点涉及前列腺素内过氧化物合酶2等,关键疾病涉及多种恶性肿瘤和炎症等。GO富集分析包含GO条目1 680个,其中生物过程相关条目1 367个,分子功能相关条目235个,细胞组分相关条目78个。KEGG通路富集分析包含KEGG通路137条,主要涉及多种恶性肿瘤信号通路和多种心血管疾病相关的信号通路。结论:本研究结果初步验证了"金银花-板蓝根"药对的基本药理作用及其机制,并为进一步深入揭示其作用机制奠定了良好基础。
基金supported by the Beijing Municipal Natural Science Foundation(Grant Nos.7092021 and 7072019)the Beijing Science and Technology Support(Grant No.Z08050703080882)the National Natural Science Foundation of China(Grant No.30571988)
文摘The miRNA expression profile was initially established to investigate its corresponding function in human uveal melanoma. The miRNA expression profile in human uveal melanoma was analyzed by a micro chip technique.The hsa-miRNA expression between four uveal melanomas and four normal uveal tissues was compared.Based on the bioinformatic approach,chip data was analyzed to select out differentially expressed candidate hsa-miRNAs.Real-time quantitative PCR(RT-PCR) was used to confirm the candidate hsa-miRNAs expression in all samples.The results of miRNA microarray chips that matched with RT-PCR were considered as the miRNA expression which was significantly different between normal tissue and uveal melanomas.In four uveal melanomas,expressions of miRNA-20a,miRNA-106a,miRNA-17,miRNA-21,and miRNA-34a were significantly up-regulated,while miRNA-145 and miRNA-204 expression were significantly down-regulated.We used miRNA microarray analysis as a fast,efficient technology to study biological information.The differentially expressed miRNAs may be involved in uveal melanoma pathogenesis,and may help promote the diagnosis and treatment for uveal melanoma.
基金Supported by the National Basic Research Program of China (Grant No. 2005CB724600)the National Natural Science Foundation of China (Grant No. 30600110)
文摘Recently, the identification of miRNA targets has received much attention. The strategies to determine miRNA targets include bioinformatic prediction and experimental assays. The bioinformatic prediction methods are mainly based on the confirmed rules of interaction between miRNAs and their targets, and are carried out by programs, such as miRanda, TargetScan, TargetScanS, RNAhybrid, DIANA-microT, PicTar, RNA22 and FindTar, which follow well-known principles. The experimental assays to find miRNA targets employ immunoprecipitation of AGO proteins to identify interacting mRNAs, or the analysis of mRNA or protein levels to identify genes which can be regulated by miRNAs. The improvement of current bioinformatic and experimental assays and the development of novel assays will enable greater efficiency in the identification of miRNA targets and thus facilitate miRNA research. This paper describes progress in the prediction and identification of miRNA targets.
