A small spring fed stream precipitates calcite by outgassing of CO<sub>2</sub> due to chemically controlled inorganic processes. The chemical composition of the water was measured along 9 stations downstre...A small spring fed stream precipitates calcite by outgassing of CO<sub>2</sub> due to chemically controlled inorganic processes. The chemical composition of the water was measured along 9 stations downstream with respect to Ca<sup>2+</sup>, Mg<sup>2+</sup>, Na<sup>+</sup>,K<sup>+</sup>, Cl<sup>-</sup>, carbonate alkalinlty, and SO<sub>4</sub><sup>2-</sup>. Temperature and pH were measured in situ. Small rectangular shaped tablets of limestone from the area were immersed into the stream for short periods and water analyses were carried out at the same time. From weight increase of the tablets, precipitation rates展开更多
目的 探讨尼可地尔片联合单硝酸异山梨酯缓释胶囊治疗不稳定型心绞痛（UAP）的临床疗效。方法 入住本院的UAP患者76例，随机分为观察组（ n ＝38）和对照组（ n ＝38）。所有患者均接受常规治疗，包括口服阿司匹林、酒石酸美托洛尔片、...目的 探讨尼可地尔片联合单硝酸异山梨酯缓释胶囊治疗不稳定型心绞痛（UAP）的临床疗效。方法 入住本院的UAP患者76例，随机分为观察组（ n ＝38）和对照组（ n ＝38）。所有患者均接受常规治疗，包括口服阿司匹林、酒石酸美托洛尔片、培哚普利、阿托伐他汀钙片等，对照组在常规治疗基础上加用单硝酸异山梨酯缓释胶囊．观察组患者在对照组基础上再联合使用尼可地尔片，治疗1个月后，观察两组临床疗效、动态心电图变化及不良反应发生率。结果 观察组治疗后的总有效率为94．7％，对照组为78．9％，两组比较有显著性差异（P ＜0．05）；观察组心电图改善情况优于对照组，差异有统计学意义（P ＜0．05）；治疗期间观察组出现轻微头痛1例（2．6％），对照组出现轻微头痛3例（7．89％），差异无统计学意义（P＞0．05）。治疗期间两组患者均无明显低血压和肝肾损害。结论 尼可地尔联合单硝酸异山梨酯可以显著改善 UAP患者的临床症状和心电图，近期疗效较好，且使用安全。展开更多
OBJECTIVE:To investigate the efficacy of Bushen Kangshuai(BS-KS)tablet on autophagy and polarization in mouse macrophage RAW 264.7.MEYHODS:Macrophage autophagy was induced by oxidized low-density lipoprotein(100μg/m ...OBJECTIVE:To investigate the efficacy of Bushen Kangshuai(BS-KS)tablet on autophagy and polarization in mouse macrophage RAW 264.7.MEYHODS:Macrophage autophagy was induced by oxidized low-density lipoprotein(100μg/m L).To detect the levels of autophagy,macrophage were transfected with double fluorescence LC3 autophagy adenovirus,then the numbers of autophagosomes and autophagic lysosomes were asessed by confocal microscopy.The autophagy related proteins expression of PI3 K,Akt,phospho-m Akt(p-Akt)and m TOR,phospho-m TOR(p-TOR),p62,microtubule-associated protein 1(LC3-Ⅱ)were determined by western blotting.The macrophage polarization model was induced by lipopolysaccharide(1μg/m L).The m RNA levels of i NOS,CD86(M1 macrophages marker molecules),and CD206,Arg-1(M2 macrophages marker molecules)were detected by real-time quantitative PCR.The concentration of cytokines TNF-αand IL-10 was determined by enzyme-linked immunosorbent assay.The protein expression of nuclear proteins PPAR-γ,NF-κB,and cytoplasmic protein IKBαwas determined by western blotting.RESULTS:The expression of the autophagy-related protein LC3-Ⅱwas increased and the expression of p62 was decreased in the BS-KS intervention group.The protein expression of PI3 K,p-Akt,and p-m TOR was also reduced.BS-KS also inhibited the m RNA expression of i NOS and CD86 on M2 macrophage,but promoted the expression of CD206 and Arg-1 on M2 macrophage.With respect to the regulation of inflammatory factors,BS-KS could inhibit the secretion of pro-inflammatory TNF-αand promote the secretion of anti-inflammatory IL-10.It also inhibited the protein expression of IKB-αand NF-κB,and promoted the expression of nuclear protein PPAR-γ.CONCLUSION:We believe that BS-KS promotes macrophage autophagy by increasing the level of autophagy protein and inhibiting the PI3 K/Akt/m TOR signaling pathway.Furthermore,BS-KS seems to inhibit macrophage M1 polarization and promote M2 polarization via the PPAR gamma/NF-κB signaling pathway,thus playing an inhibitory role in atheros展开更多
文摘A small spring fed stream precipitates calcite by outgassing of CO<sub>2</sub> due to chemically controlled inorganic processes. The chemical composition of the water was measured along 9 stations downstream with respect to Ca<sup>2+</sup>, Mg<sup>2+</sup>, Na<sup>+</sup>,K<sup>+</sup>, Cl<sup>-</sup>, carbonate alkalinlty, and SO<sub>4</sub><sup>2-</sup>. Temperature and pH were measured in situ. Small rectangular shaped tablets of limestone from the area were immersed into the stream for short periods and water analyses were carried out at the same time. From weight increase of the tablets, precipitation rates
基金Supported by National Natural Science Foundation of China(Effect of Bushen Kangshuai Tablet on Early Atherosclerosis from Rho/ROCK Pathway Based on Adventitia DamageNo.81173244)the Project Funding given to the Second Batch of National"Ten Thousand People Plan"Million Project Leader(No.20160621).
文摘OBJECTIVE:To investigate the efficacy of Bushen Kangshuai(BS-KS)tablet on autophagy and polarization in mouse macrophage RAW 264.7.MEYHODS:Macrophage autophagy was induced by oxidized low-density lipoprotein(100μg/m L).To detect the levels of autophagy,macrophage were transfected with double fluorescence LC3 autophagy adenovirus,then the numbers of autophagosomes and autophagic lysosomes were asessed by confocal microscopy.The autophagy related proteins expression of PI3 K,Akt,phospho-m Akt(p-Akt)and m TOR,phospho-m TOR(p-TOR),p62,microtubule-associated protein 1(LC3-Ⅱ)were determined by western blotting.The macrophage polarization model was induced by lipopolysaccharide(1μg/m L).The m RNA levels of i NOS,CD86(M1 macrophages marker molecules),and CD206,Arg-1(M2 macrophages marker molecules)were detected by real-time quantitative PCR.The concentration of cytokines TNF-αand IL-10 was determined by enzyme-linked immunosorbent assay.The protein expression of nuclear proteins PPAR-γ,NF-κB,and cytoplasmic protein IKBαwas determined by western blotting.RESULTS:The expression of the autophagy-related protein LC3-Ⅱwas increased and the expression of p62 was decreased in the BS-KS intervention group.The protein expression of PI3 K,p-Akt,and p-m TOR was also reduced.BS-KS also inhibited the m RNA expression of i NOS and CD86 on M2 macrophage,but promoted the expression of CD206 and Arg-1 on M2 macrophage.With respect to the regulation of inflammatory factors,BS-KS could inhibit the secretion of pro-inflammatory TNF-αand promote the secretion of anti-inflammatory IL-10.It also inhibited the protein expression of IKB-αand NF-κB,and promoted the expression of nuclear protein PPAR-γ.CONCLUSION:We believe that BS-KS promotes macrophage autophagy by increasing the level of autophagy protein and inhibiting the PI3 K/Akt/m TOR signaling pathway.Furthermore,BS-KS seems to inhibit macrophage M1 polarization and promote M2 polarization via the PPAR gamma/NF-κB signaling pathway,thus playing an inhibitory role in atheros
