Objective：To investigate the effect of blood-activating Chinese medicinal compounds and waterdraining Chinese medicinal compounds on tumor necrosis factor alpha（TNF-α）and nuclear factor kappaB（NF-κB）expressions...Objective：To investigate the effect of blood-activating Chinese medicinal compounds and waterdraining Chinese medicinal compounds on tumor necrosis factor alpha（TNF-α）and nuclear factor kappaB（NF-κB）expressions in rats with intracerebral hemorrhage（ICH）at the acute stage,and to monitor their therapeutic effect and mechanism of action on inflammation and cerebral edema.Methods：A rat model of cerebral hemorrhage was achieved by injecting autologous arterial blood into the caudate nucleus.A total of 168 rats were randomly divided into 4 groups：blood-activating medicine group（n=42）,water-draining medicine group（n=42）,sham operated group（n=42）,and the model group（n=42）.A series of brain samples were obtained at days 1,3 and 5 after ICH from rats in all groups.Protein expression levels of TNF-αand NF-κB were measured by immunohistochemical staining and gene expression levels of TNF-αand NF-κB were measured by real-time fluorescent PCR.Results：Compared to the sham operated group,protein expression levels of TNF-αand NF-κB in the model group significantly increased（P〈0.01）.Protein and gene expressions of TNF-αfrom the blood-activating medicine group and water-draining medicine group significantly decreased when compared to those in the model group（P〈0.05）.Meanwhile,compared to the model group,the expression of NF-κB in the blood-activating medicine group significantly decreased（P〈0.05）,while expression of NF-κB in the water-draining medicine group did not differ（P〉0.05）.Conclusions：Blood-activating Chinese medicinal compounds and water-draining Chinese medicinal compounds can alleviate inflammation of peripheral tissue and cerebral edema.However,the blood-activating Chinese medicinal compounds were more effective than the water-draining Chinese medicinal compounds.The possible effective mechanism may be by means of inhibiting the activation of NF-κB so as to suppress the transcription of target genes including gene expression of TNF-α.展开更多
文摘【目的】探讨手足口病不同临床分期（1、2、3、4期）肿瘤坏死因子（tumor necrosis factor ，TNF）、白介素-6（IL-6）及B型脑钠肽（BNP）的变化特点，为临床评估此类患者提供参考。【方法】选择2011年5月至2013年10月在本院就诊的手足口病患儿292例（其中1期156例，2期106例，3期20例，4期10例），于患儿入院24 h内抽血行TNF、IL-6、BNP检测。观察比较不同分期手足口病患儿细胞因子及BNP的变化特点。【结果】各期TNF、IL-6、BNP水平均较健康对照组明显增高，2期、3期患儿TNF、IL-6、BNP水平明显高于1期，4期的TNF、IL-6、BNP水平明显高于2期、3期水平，且差异相比较均有显著性（ P ＜0．05）。2、3期TNF、IL-6、BNP水平相比较差异无显著性（ P ＞0．05）。【结论】随着患儿病情加重，TNF、IL-6及BNP水平随之相应增高，TNF、IL-6及BN P水平可用于手足口病患儿病情评估的参考指标。
基金Supported by the National Natural Science Foundation of China(No.30873208)
文摘Objective：To investigate the effect of blood-activating Chinese medicinal compounds and waterdraining Chinese medicinal compounds on tumor necrosis factor alpha（TNF-α）and nuclear factor kappaB（NF-κB）expressions in rats with intracerebral hemorrhage（ICH）at the acute stage,and to monitor their therapeutic effect and mechanism of action on inflammation and cerebral edema.Methods：A rat model of cerebral hemorrhage was achieved by injecting autologous arterial blood into the caudate nucleus.A total of 168 rats were randomly divided into 4 groups：blood-activating medicine group（n=42）,water-draining medicine group（n=42）,sham operated group（n=42）,and the model group（n=42）.A series of brain samples were obtained at days 1,3 and 5 after ICH from rats in all groups.Protein expression levels of TNF-αand NF-κB were measured by immunohistochemical staining and gene expression levels of TNF-αand NF-κB were measured by real-time fluorescent PCR.Results：Compared to the sham operated group,protein expression levels of TNF-αand NF-κB in the model group significantly increased（P〈0.01）.Protein and gene expressions of TNF-αfrom the blood-activating medicine group and water-draining medicine group significantly decreased when compared to those in the model group（P〈0.05）.Meanwhile,compared to the model group,the expression of NF-κB in the blood-activating medicine group significantly decreased（P〈0.05）,while expression of NF-κB in the water-draining medicine group did not differ（P〉0.05）.Conclusions：Blood-activating Chinese medicinal compounds and water-draining Chinese medicinal compounds can alleviate inflammation of peripheral tissue and cerebral edema.However,the blood-activating Chinese medicinal compounds were more effective than the water-draining Chinese medicinal compounds.The possible effective mechanism may be by means of inhibiting the activation of NF-κB so as to suppress the transcription of target genes including gene expression of TNF-α.
