AIM To assess the possible roles of cytokines (TNF α, IFN γ, IL 6 and IL 8) in liver damage of hepatitis B. METHODS The serum TNF α, IFN γ, IL 6 and IL 8 were detected by ELISA in 66 patients with hepat...AIM To assess the possible roles of cytokines (TNF α, IFN γ, IL 6 and IL 8) in liver damage of hepatitis B. METHODS The serum TNF α, IFN γ, IL 6 and IL 8 were detected by ELISA in 66 patients with hepatitis B and 20 healthy blood donors. RESULTS TNF α and IL 6 in all types of clinical hepatitis B were significantly higher than those in healthy blood donors ( P <0 05); meanwhile the levels of TNF α, IFN γ, IL 6 and IL 8 in the patients with fulminant hepatitis B were much higher than those in the patients with acute hepatitis B ( P <0 05); the level of TNF α was positively correlated with the levels of IFN γ, Il 6 and IL 8 in all types of hepatitis B ( r IFN =0 24, r IL 6 =0 35, r IL 8 =0 44) and the TNF α, IFN γ, IL 6 and IL 8 were positively correlated with serum bilirubin ( P <0 05). Dynamic changes of these cytokines were observed in the course of acute and fulminant hepatitis. The level of IFN γ peaked in the initial period of acute hepatitis and early stage of hepatic coma in fulminant hepatitis; TNF α, IL 6 and IL 8 increased with exacerbation, and reached a peak when the liver damage was most serious, then decreased when patient conditions were improved. CONCLUSION The increased cytokines were related to the inflammation of liver cells and multiple factors may play certain roles in liver damage.展开更多
Gastrodin, the major component isolated from the rhizome of the Chinese traditional medicinal herb Gastrodia elata（‘‘Tianma＇＇）, has a long history in the treatment of epilepsy and other neurological disorders.Ho...Gastrodin, the major component isolated from the rhizome of the Chinese traditional medicinal herb Gastrodia elata（‘‘Tianma＇＇）, has a long history in the treatment of epilepsy and other neurological disorders.However, the molecular mechanisms are not clear. Here,we found that gastrodin ameliorated pentylenetetrazole（PTZ）-induced epileptic seizures with improvement of the electroencephalographic pattern in mice. Further studies demonstrated that gastrodin decreased the levels of the pro-inflammatory cytokines interleukin-1b and tumor necrosis factor-a while increasing interleukin-10, an antiinflammatory cytokine in the brain. Furthermore, gastrodin attenuated the PTZ-induced microglial activation along with inhibition of mitogen-activated protein kinases,c AMP response element binding protein, and NF-j B. Our data suggest that gastrodin attenuates seizures by modulating the mitogen-activated protein kinase-associated inflammatory responses.展开更多
文摘AIM To assess the possible roles of cytokines (TNF α, IFN γ, IL 6 and IL 8) in liver damage of hepatitis B. METHODS The serum TNF α, IFN γ, IL 6 and IL 8 were detected by ELISA in 66 patients with hepatitis B and 20 healthy blood donors. RESULTS TNF α and IL 6 in all types of clinical hepatitis B were significantly higher than those in healthy blood donors ( P <0 05); meanwhile the levels of TNF α, IFN γ, IL 6 and IL 8 in the patients with fulminant hepatitis B were much higher than those in the patients with acute hepatitis B ( P <0 05); the level of TNF α was positively correlated with the levels of IFN γ, Il 6 and IL 8 in all types of hepatitis B ( r IFN =0 24, r IL 6 =0 35, r IL 8 =0 44) and the TNF α, IFN γ, IL 6 and IL 8 were positively correlated with serum bilirubin ( P <0 05). Dynamic changes of these cytokines were observed in the course of acute and fulminant hepatitis. The level of IFN γ peaked in the initial period of acute hepatitis and early stage of hepatic coma in fulminant hepatitis; TNF α, IL 6 and IL 8 increased with exacerbation, and reached a peak when the liver damage was most serious, then decreased when patient conditions were improved. CONCLUSION The increased cytokines were related to the inflammation of liver cells and multiple factors may play certain roles in liver damage.
文摘Gastrodin, the major component isolated from the rhizome of the Chinese traditional medicinal herb Gastrodia elata（‘‘Tianma＇＇）, has a long history in the treatment of epilepsy and other neurological disorders.However, the molecular mechanisms are not clear. Here,we found that gastrodin ameliorated pentylenetetrazole（PTZ）-induced epileptic seizures with improvement of the electroencephalographic pattern in mice. Further studies demonstrated that gastrodin decreased the levels of the pro-inflammatory cytokines interleukin-1b and tumor necrosis factor-a while increasing interleukin-10, an antiinflammatory cytokine in the brain. Furthermore, gastrodin attenuated the PTZ-induced microglial activation along with inhibition of mitogen-activated protein kinases,c AMP response element binding protein, and NF-j B. Our data suggest that gastrodin attenuates seizures by modulating the mitogen-activated protein kinase-associated inflammatory responses.
