目的对筛选出的食管鳞癌组织中的差异蛋白平滑肌肌动蛋白22α(Transgelin,TAGLN)及其异构体肌动蛋白结合蛋白2(Transgelin2,TAGLN2)进行验证。方法收集新疆医科大学第一附属医院2014年1月-2015年8月食管鳞状细胞癌患者新鲜癌组织及相应...目的对筛选出的食管鳞癌组织中的差异蛋白平滑肌肌动蛋白22α(Transgelin,TAGLN)及其异构体肌动蛋白结合蛋白2(Transgelin2,TAGLN2)进行验证。方法收集新疆医科大学第一附属医院2014年1月-2015年8月食管鳞状细胞癌患者新鲜癌组织及相应癌旁正常黏膜组织标本共28对;按照民族及淋巴结是否发生转移进行分组,运用Western Blot技术对新鲜食管鳞癌组织中的TAGLN、TAGLN2蛋白表达情况进行半定量分析。结果 Western Blot技术半定量分析发现TAGLN、TAGLN2在食管鳞状细胞癌肿瘤组织中的表达高于相应癌旁正常组织(P<0.05);TAGLN在食管鳞状细胞癌淋巴结转移组中的表达高于淋巴结未转移组(P<0.05)。结论TAGLN、TAGLN2是食管鳞状细胞癌发生、发展过程中的重要变化分子。展开更多
上皮间质转化（epithelial-mesenchymal transition,EMT）使上皮细胞极性和细胞间紧密连接消失,并赋予肿瘤细胞侵袭和转移的能力。本研究应用携带TAGLN2特异性沉默序列或无关序列的慢病毒载体感染食管鳞癌细胞系EC9706,建立EC9706 sh TA...上皮间质转化（epithelial-mesenchymal transition,EMT）使上皮细胞极性和细胞间紧密连接消失,并赋予肿瘤细胞侵袭和转移的能力。本研究应用携带TAGLN2特异性沉默序列或无关序列的慢病毒载体感染食管鳞癌细胞系EC9706,建立EC9706 sh TAGLN2及阴性对照EC9706 sh Control亚细胞系。以5~80 ng/m L不同浓度或40 ng/m L TGF-β刺激EC9706 sh TAGLN2和EC9706sh Control细胞,发现与EC9706 sh Control细胞比较,EC9706 sh TAGLN2细胞中TAGLN2的表达无明显增加,同时TGF-β诱导的间质标志物如N-cadherin、Vimentin等蛋白质表达无明显升高;上皮标志物如E-cadherin表达无明显降低;TAGLN2的沉默不仅阻断了TGF-β诱导EC9706 sh TAGLN2细胞体外迁移和侵袭能力的增加,并抑制TGF-β信号通路下游分子p-Smad2/3的激活。本研究结果提示,TGF-β可能通过TAGLN2促进食管鳞癌的恶性演进,TAGLN2是潜在的食管鳞癌治疗的分子靶点之一。展开更多
Objective To examine the expression and regulation of Tagln2 gene in rabbit uterus during early pregnancy. Methods In situ hybridization was used to detect the expression of Tagln2 mRNA in rabbit uterus during early p...Objective To examine the expression and regulation of Tagln2 gene in rabbit uterus during early pregnancy. Methods In situ hybridization was used to detect the expression of Tagln2 mRNA in rabbit uterus during early pregnancy and their regulation under pseudopregnancy and hormonal treatment. Results In situ hybridization analysis revealed Tagln2 mRNA expression in a spatiotemporally regulated pattern in early pregnant uterus. Tagln2 mRNA was highly expressed in the uterine epithelium from days 2 to 4. A low expression level was detected in the glandular epithelium near the myometrium on days 5 and 6. On days 7.0 and 7.25, Tagln2 expression was upregulated at implantation sites and mainly expressed in luminal epithelium and blastocyst at the anti-mesometrial and lateral sides. On day 8, Tagln2 expression was mainly detected in fusion area and glandular epithelium at anti-mesometrial sides, and became stronger in trophectoderm and luminal epithelium at mesometrial side. Tagln2 expression was not detected in inter-implantation sites from days 6 to 8 of pregnancy and from days 6 to 8 of peudopregnancy. In the ovariectomized rabbit uteri, Tagln2 expression was upregulated by either estrogen or progesterone. Conclusion Tagln2 was highly expressed in the endometrium at implantation sites and need actived embryo, upregulated by steroid hormones.展开更多
文摘目的对筛选出的食管鳞癌组织中的差异蛋白平滑肌肌动蛋白22α(Transgelin,TAGLN)及其异构体肌动蛋白结合蛋白2(Transgelin2,TAGLN2)进行验证。方法收集新疆医科大学第一附属医院2014年1月-2015年8月食管鳞状细胞癌患者新鲜癌组织及相应癌旁正常黏膜组织标本共28对;按照民族及淋巴结是否发生转移进行分组,运用Western Blot技术对新鲜食管鳞癌组织中的TAGLN、TAGLN2蛋白表达情况进行半定量分析。结果 Western Blot技术半定量分析发现TAGLN、TAGLN2在食管鳞状细胞癌肿瘤组织中的表达高于相应癌旁正常组织(P<0.05);TAGLN在食管鳞状细胞癌淋巴结转移组中的表达高于淋巴结未转移组(P<0.05)。结论TAGLN、TAGLN2是食管鳞状细胞癌发生、发展过程中的重要变化分子。
文摘上皮间质转化（epithelial-mesenchymal transition,EMT）使上皮细胞极性和细胞间紧密连接消失,并赋予肿瘤细胞侵袭和转移的能力。本研究应用携带TAGLN2特异性沉默序列或无关序列的慢病毒载体感染食管鳞癌细胞系EC9706,建立EC9706 sh TAGLN2及阴性对照EC9706 sh Control亚细胞系。以5~80 ng/m L不同浓度或40 ng/m L TGF-β刺激EC9706 sh TAGLN2和EC9706sh Control细胞,发现与EC9706 sh Control细胞比较,EC9706 sh TAGLN2细胞中TAGLN2的表达无明显增加,同时TGF-β诱导的间质标志物如N-cadherin、Vimentin等蛋白质表达无明显升高;上皮标志物如E-cadherin表达无明显降低;TAGLN2的沉默不仅阻断了TGF-β诱导EC9706 sh TAGLN2细胞体外迁移和侵袭能力的增加,并抑制TGF-β信号通路下游分子p-Smad2/3的激活。本研究结果提示,TGF-β可能通过TAGLN2促进食管鳞癌的恶性演进,TAGLN2是潜在的食管鳞癌治疗的分子靶点之一。
文摘Objective To examine the expression and regulation of Tagln2 gene in rabbit uterus during early pregnancy. Methods In situ hybridization was used to detect the expression of Tagln2 mRNA in rabbit uterus during early pregnancy and their regulation under pseudopregnancy and hormonal treatment. Results In situ hybridization analysis revealed Tagln2 mRNA expression in a spatiotemporally regulated pattern in early pregnant uterus. Tagln2 mRNA was highly expressed in the uterine epithelium from days 2 to 4. A low expression level was detected in the glandular epithelium near the myometrium on days 5 and 6. On days 7.0 and 7.25, Tagln2 expression was upregulated at implantation sites and mainly expressed in luminal epithelium and blastocyst at the anti-mesometrial and lateral sides. On day 8, Tagln2 expression was mainly detected in fusion area and glandular epithelium at anti-mesometrial sides, and became stronger in trophectoderm and luminal epithelium at mesometrial side. Tagln2 expression was not detected in inter-implantation sites from days 6 to 8 of pregnancy and from days 6 to 8 of peudopregnancy. In the ovariectomized rabbit uteri, Tagln2 expression was upregulated by either estrogen or progesterone. Conclusion Tagln2 was highly expressed in the endometrium at implantation sites and need actived embryo, upregulated by steroid hormones.
