With the increasing demand for multiplex and high-throughput analysis of large numbers of biomolecules,multiplex technology becomes a promising tool for carrying out thousands of individual reactions at the same time ...With the increasing demand for multiplex and high-throughput analysis of large numbers of biomolecules,multiplex technology becomes a promising tool for carrying out thousands of individual reactions at the same time for large-scale biological analysis. Among current technologies,suspension arrays based on appropriate barcode particles have been popularly used in multiplex bioassays of many research fields with the ability of unique encoding, such as in the clinical, medicinal, nutritional, and environmental fields.Besides the unique form of barcode, these particles have higher flexibility, better sensitivity, and faster reaction kinetics. In this review, we present some examples of typical barcode particles that are divided into different groups depending on how they are encoded and their applications in multiplex bioassays for different targets such as proteins,DNA and RNA sequences, and cells. The bioassays for monitoring food safety, drug research, and clinical diagnosis are also described.展开更多
A closed-loop experimental system is established to investigate the heat transfer characteristics of microcapsule phase change material(MEPCM) suspension in an array jet impingement. Eicosane with a melting peak at 4...A closed-loop experimental system is established to investigate the heat transfer characteristics of microcapsule phase change material(MEPCM) suspension in an array jet impingement. Eicosane with a melting peak at 40.8℃ is used as the capsule core of the MEPCM particle. Five kinds of array-hole nozzles with the same hole cross-sectional area are employed to analyze the influence of critical parameters, including the nozzle hole number, hole spacing, impinging distance, and jet temperature. It shows that a 5% suspension may improve the heat transfer coefficient of the array jet by up to 23.5% compared with water. The heat transfer of an array jet is obviously stronger than that of a single jet, but too much hole number is not conducive because of the entrainment interference between adjacent jets. A larger hole spacing or smaller impinging distance may weaken the crossflow accumulation on the impinged surface, thus enhancing the heat transfer capability. The heat transfer coefficient of the array jet presents a secondary peak value at the end of the jet-core region. The latent heat absorption of the capsule core results in superior heat transfer of the suspension compared to that of water only in a specific range of jet temperatures, the optimum of which is approximately 10℃ lower relative to the peak melting temperature. In addition, the melt completion time of a single MEPCM particle and the critical flow rate of the suspension are predicted theoretically.展开更多
基金supported by the National Natural Science Foundation of China (21473029 and 51522302)the NSAF Foundation of China (U1530260)+3 种基金the Key Medical Projects of Jiangsu Province ( BL2014078)Key Discipline of Jiangsu Province (2016-2020)the Natural Science Foundation of Jiangsu (BK20140028)the Scientific Research Foundation of Southeast University
文摘With the increasing demand for multiplex and high-throughput analysis of large numbers of biomolecules,multiplex technology becomes a promising tool for carrying out thousands of individual reactions at the same time for large-scale biological analysis. Among current technologies,suspension arrays based on appropriate barcode particles have been popularly used in multiplex bioassays of many research fields with the ability of unique encoding, such as in the clinical, medicinal, nutritional, and environmental fields.Besides the unique form of barcode, these particles have higher flexibility, better sensitivity, and faster reaction kinetics. In this review, we present some examples of typical barcode particles that are divided into different groups depending on how they are encoded and their applications in multiplex bioassays for different targets such as proteins,DNA and RNA sequences, and cells. The bioassays for monitoring food safety, drug research, and clinical diagnosis are also described.
基金supported by the National Natural Science Foundation of China (Grant No. 51706150)National Key R&D Program of China(Grant No. 2020YFB0606302)。
文摘A closed-loop experimental system is established to investigate the heat transfer characteristics of microcapsule phase change material(MEPCM) suspension in an array jet impingement. Eicosane with a melting peak at 40.8℃ is used as the capsule core of the MEPCM particle. Five kinds of array-hole nozzles with the same hole cross-sectional area are employed to analyze the influence of critical parameters, including the nozzle hole number, hole spacing, impinging distance, and jet temperature. It shows that a 5% suspension may improve the heat transfer coefficient of the array jet by up to 23.5% compared with water. The heat transfer of an array jet is obviously stronger than that of a single jet, but too much hole number is not conducive because of the entrainment interference between adjacent jets. A larger hole spacing or smaller impinging distance may weaken the crossflow accumulation on the impinged surface, thus enhancing the heat transfer capability. The heat transfer coefficient of the array jet presents a secondary peak value at the end of the jet-core region. The latent heat absorption of the capsule core results in superior heat transfer of the suspension compared to that of water only in a specific range of jet temperatures, the optimum of which is approximately 10℃ lower relative to the peak melting temperature. In addition, the melt completion time of a single MEPCM particle and the critical flow rate of the suspension are predicted theoretically.
