目的探讨犬快速右心房起搏诱发房颤48 h及8周后,心房肌结构重构包括心肌组织形态、糖原聚集情况和超微结构的变化及左、右心房变化的不均一性。方法成年比格犬在全麻下用快速右心房起搏(600次/min)建立房颤模型,对比格犬进行分组,分为...目的探讨犬快速右心房起搏诱发房颤48 h及8周后,心房肌结构重构包括心肌组织形态、糖原聚集情况和超微结构的变化及左、右心房变化的不均一性。方法成年比格犬在全麻下用快速右心房起搏(600次/min)建立房颤模型,对比格犬进行分组,分为对照组(control组)、48 h房颤组(48 h AF组)、8周房颤组(8 w AF组),每组各6只,分别取左右心房肌组织,行HE染色、糖原染色、马松(Masson)染色及透射电镜检查。观察随着房颤持续时间的延长,左、右心房组织大体结构,糖原沉积纤维化程度及超微结构的变化。结果随着房颤时间的延长,心房肌逐渐出现心肌纤维排列紊乱,心肌细胞数目减少,结缔组织增生。control组左房糖原累积程度高于右房,差异具有统计学意义(P<0.05),在48 h AF组及8 w AF组糖原沉积及纤维化较control组明显增加,8 w AF组较48 h AF组更为明显,差异具有统计学意义(P<0.05)。随着房颤时间的延长,心房肌逐渐出现线粒体肿胀变长,体积增大,基质变淡,线粒体脊密度减低,肌丝排列紊乱。闰盘扭曲模糊、不连续。结论随着房颤持续时间的延长,心房肌组织逐渐出现结构重构,心肌细胞排列紊乱,糖原聚集,线粒体异常及闰盘排列紊乱,为房颤的维持提供了基质。展开更多
To examine the effects of prenatal tetrandrine (Tet) therapy on pulmonary arterial structural remodeling in nitrofen-induced congenital diaphragmatic hernia (CDH) Methods CDH was induced in fetal rats by materna...To examine the effects of prenatal tetrandrine (Tet) therapy on pulmonary arterial structural remodeling in nitrofen-induced congenital diaphragmatic hernia (CDH) Methods CDH was induced in fetal rats by maternal administration of 100*!mg nitrofen by gavage on day 9 5 of gestation (term, day 22) Control animals received olive oil (OO) Tet (24*!mg/kg per day) or normal saline (NS) was given by gavage every day from 16 to 20 days of gestation, and fetuses were delivered by caesarean section on day 21 5 Lung sections from 3 fetuses in each group were studied The number of vessels were calculated, the external diameter (ED), medial wall thickness (MT), percent of medial wall thickness, and wall structure were evaluated by image analysis software Results In the pre-acinar arteries, CDH-NS pups had a significantly increased %MT compared with the OO-NS group ( P <0 05), while CDH-Tet animals had a reduced %MT compared with the CDH-NS rats ( P <0 05) Similar results were seen in the intra-acinar level Significant differences were observed between CDH-NS animals and OO-NS controls in the percentage of muscularized intra-acinar blood vessels ( P <0 001) Tet-treated CDH pups had a reduced percentage of muscularized intra-acinar arteries compared with CDH-NS animals Conclusions Medial hypertrophy is present in both the pre-acinar and intra-acinar arteries in the nitrofen-induced CDH rat model Tet treatment inhibits medial hypertrophy and reduces the percentage of muscularized intra-acinar vessels Prenatal Tet therapy may be efficacious in reducing the risk of PH in human newborns with CDH展开更多
文摘目的探讨犬快速右心房起搏诱发房颤48 h及8周后,心房肌结构重构包括心肌组织形态、糖原聚集情况和超微结构的变化及左、右心房变化的不均一性。方法成年比格犬在全麻下用快速右心房起搏(600次/min)建立房颤模型,对比格犬进行分组,分为对照组(control组)、48 h房颤组(48 h AF组)、8周房颤组(8 w AF组),每组各6只,分别取左右心房肌组织,行HE染色、糖原染色、马松(Masson)染色及透射电镜检查。观察随着房颤持续时间的延长,左、右心房组织大体结构,糖原沉积纤维化程度及超微结构的变化。结果随着房颤时间的延长,心房肌逐渐出现心肌纤维排列紊乱,心肌细胞数目减少,结缔组织增生。control组左房糖原累积程度高于右房,差异具有统计学意义(P<0.05),在48 h AF组及8 w AF组糖原沉积及纤维化较control组明显增加,8 w AF组较48 h AF组更为明显,差异具有统计学意义(P<0.05)。随着房颤时间的延长,心房肌逐渐出现线粒体肿胀变长,体积增大,基质变淡,线粒体脊密度减低,肌丝排列紊乱。闰盘扭曲模糊、不连续。结论随着房颤持续时间的延长,心房肌组织逐渐出现结构重构,心肌细胞排列紊乱,糖原聚集,线粒体异常及闰盘排列紊乱,为房颤的维持提供了基质。
基金ThissubjectwassupportedbytheFoundationofScienceandTechnologyCommitteeofSichuanProvince (No G970 15 )
文摘To examine the effects of prenatal tetrandrine (Tet) therapy on pulmonary arterial structural remodeling in nitrofen-induced congenital diaphragmatic hernia (CDH) Methods CDH was induced in fetal rats by maternal administration of 100*!mg nitrofen by gavage on day 9 5 of gestation (term, day 22) Control animals received olive oil (OO) Tet (24*!mg/kg per day) or normal saline (NS) was given by gavage every day from 16 to 20 days of gestation, and fetuses were delivered by caesarean section on day 21 5 Lung sections from 3 fetuses in each group were studied The number of vessels were calculated, the external diameter (ED), medial wall thickness (MT), percent of medial wall thickness, and wall structure were evaluated by image analysis software Results In the pre-acinar arteries, CDH-NS pups had a significantly increased %MT compared with the OO-NS group ( P <0 05), while CDH-Tet animals had a reduced %MT compared with the CDH-NS rats ( P <0 05) Similar results were seen in the intra-acinar level Significant differences were observed between CDH-NS animals and OO-NS controls in the percentage of muscularized intra-acinar blood vessels ( P <0 001) Tet-treated CDH pups had a reduced percentage of muscularized intra-acinar arteries compared with CDH-NS animals Conclusions Medial hypertrophy is present in both the pre-acinar and intra-acinar arteries in the nitrofen-induced CDH rat model Tet treatment inhibits medial hypertrophy and reduces the percentage of muscularized intra-acinar vessels Prenatal Tet therapy may be efficacious in reducing the risk of PH in human newborns with CDH
