钙依赖性蛋白激酶(calcium-dependent protein kinases,CDPKs or CPKs)作为一类钙感知蛋白在植物的生长发育和胁迫应答中起着重要的作用。LeCPK2(GenBank accession No.:GQ205414)是我们从番茄中分离的第3个CDPK基因,前期研究表明LeCPK...钙依赖性蛋白激酶(calcium-dependent protein kinases,CDPKs or CPKs)作为一类钙感知蛋白在植物的生长发育和胁迫应答中起着重要的作用。LeCPK2(GenBank accession No.:GQ205414)是我们从番茄中分离的第3个CDPK基因,前期研究表明LeCPK2可能在植物热胁迫应答中发挥作用。为了进一步研究其在热胁迫中的功能,我们通过电子克隆的方法分离了LeCPK2的启动子序列,并通过LeCPK2过表达烟草分析其在高温胁迫中的潜在的功能。生物信息学分析显示,LeCPK2启动子中包含5个热响应元件,和前期试验结果一致。野生型植株在受到热胁迫后,对光更为敏感,强光照下植株叶片发生萎蔫,而强光本身不会对未受热胁迫的健康植株造成伤害。LeCPK2转基因植株热、光胁迫后不会出现受害表型。以上研究表明,LeCPK2在植物的热胁迫应答中发挥重要作用,能够有效保护植株免受高温胁迫的损害,是一个优秀的耐热(光)基因。本研究将为揭示番茄LeCPK2遗传功能及对其开发利用奠定基础。展开更多
Low frequency flicker,high frequency flicker,strong light,strong blue light,infrared,ultraviolet,electromagnetic radiation,ripple flicker and dimming flicker produced by different lamps have negative impact on vision,...Low frequency flicker,high frequency flicker,strong light,strong blue light,infrared,ultraviolet,electromagnetic radiation,ripple flicker and dimming flicker produced by different lamps have negative impact on vision,eyes and health. Negative impact on eyes resulting in myopia or cataract etc: the solution is to remove all the negative factors by applying upright lighting technology and that is optimum to vision,eyes and health.展开更多
文摘钙依赖性蛋白激酶(calcium-dependent protein kinases,CDPKs or CPKs)作为一类钙感知蛋白在植物的生长发育和胁迫应答中起着重要的作用。LeCPK2(GenBank accession No.:GQ205414)是我们从番茄中分离的第3个CDPK基因,前期研究表明LeCPK2可能在植物热胁迫应答中发挥作用。为了进一步研究其在热胁迫中的功能,我们通过电子克隆的方法分离了LeCPK2的启动子序列,并通过LeCPK2过表达烟草分析其在高温胁迫中的潜在的功能。生物信息学分析显示,LeCPK2启动子中包含5个热响应元件,和前期试验结果一致。野生型植株在受到热胁迫后,对光更为敏感,强光照下植株叶片发生萎蔫,而强光本身不会对未受热胁迫的健康植株造成伤害。LeCPK2转基因植株热、光胁迫后不会出现受害表型。以上研究表明,LeCPK2在植物的热胁迫应答中发挥重要作用,能够有效保护植株免受高温胁迫的损害,是一个优秀的耐热(光)基因。本研究将为揭示番茄LeCPK2遗传功能及对其开发利用奠定基础。
文摘Low frequency flicker,high frequency flicker,strong light,strong blue light,infrared,ultraviolet,electromagnetic radiation,ripple flicker and dimming flicker produced by different lamps have negative impact on vision,eyes and health. Negative impact on eyes resulting in myopia or cataract etc: the solution is to remove all the negative factors by applying upright lighting technology and that is optimum to vision,eyes and health.
