目的探讨移植基质细胞衍生因子1(SDF-1)预处理的骨髓间充质干细胞(BMSC)对大鼠颈动脉狭窄的作用。方法携带增强型绿色荧光蛋白(EGFP)的质粒转染BMSC后经静脉注入大鼠。分为颈动脉损伤模型对照组、BMSC移植组、SDF-1预处理的BMSC移植组...目的探讨移植基质细胞衍生因子1(SDF-1)预处理的骨髓间充质干细胞(BMSC)对大鼠颈动脉狭窄的作用。方法携带增强型绿色荧光蛋白(EGFP)的质粒转染BMSC后经静脉注入大鼠。分为颈动脉损伤模型对照组、BMSC移植组、SDF-1预处理的BMSC移植组。细胞移植术后2周,取损伤处血管组织采用免疫荧光技术检测EGFP表达明确BMSC归巢情况;移植术后4周, Evans蓝染色观察损伤内膜内皮化程度,免疫荧光技术检测损伤血管CD31的表达情况;HE染色检测损伤颈动脉新生内膜增生程度,免疫组织化学染色法检测损伤血管增殖细胞核抗原(PCNA)的表达, Western blot法检测血管内皮生长因子(VEGF)的蛋白水平。结果移植SDF-1预处理的BMSC能有效促进干细胞向损伤血管的归巢,并促进损伤血管再内皮化程度。BMSC移植后新生内膜面积、新生内膜面积/中膜面积均低于对照组, SDF-1预处理的BMSC移植组差异更为显著。移植SDF-1预处理的BMSC可显著提高损伤内膜CD31以及VEGF的水平,并抑制PCNA的表达。结论SDF-1预处理BMSC移植可通过促进BMSC归巢至损伤处并诱导细胞分化,抑制中膜平滑肌细胞增殖来减轻血管狭窄程度。展开更多
This study was designed to determine the levels of early endothelial progenitor cells （EPCs）, apelin, vascu- lar endothelial growth factor （VEGF） and stromal cell-derived growth factor-1 （SDF-1） after acute myoc...This study was designed to determine the levels of early endothelial progenitor cells （EPCs）, apelin, vascu- lar endothelial growth factor （VEGF） and stromal cell-derived growth factor-1 （SDF-1） after acute myocardial infarction （AMI）, and to investigate the relationships between these cytokines and early EPCs. Early EPCs, de- fined as CD133＋, KDR＋, and CD34~ cells, were quantified by flow cytometry. The levels of early EPCs and those cytokines in AMI patients were significantly different from those with coronary artery disease or controls （P 〈 0.05）. Plasma apelin levels were inversely correlated with Gensini score and early EPCs （both P 〈 0.01）. Early EPCs, VEGF and SDF-1 showed different patterns of changes in AMI patients during the first 24 h. The trend in the change of early EPCs was proportionally correlated with that of VEGF （P 〈 0.05）. AMI patients exhibited in- creased early EPCs with remarkably decreased apelin levels and enhanced VEGF levels.展开更多
目的:观察独活续断汤口服和离子导入治疗肝肾亏虚型膝骨关节炎(KOA)的临床疗效及对基质细胞衍生因子-1(SDF-1)/C-X-C趋化因子受体4型(CXCR4)信号通路的影响。方法:将150例天津中医药大学第一附属医院确诊的肝肾亏虚型KOA患者随机分为对...目的:观察独活续断汤口服和离子导入治疗肝肾亏虚型膝骨关节炎(KOA)的临床疗效及对基质细胞衍生因子-1(SDF-1)/C-X-C趋化因子受体4型(CXCR4)信号通路的影响。方法:将150例天津中医药大学第一附属医院确诊的肝肾亏虚型KOA患者随机分为对照组、中药口服组和中药离子导入组,每组各50例,对照组给予硫酸氨基葡萄糖胶囊,口服0.5 g/次,2次/d口服,中药口服组给予独活续断汤150 m L/次,2次/d口服,中药离子导入组给予独活续断汤,于髋骨穴、膝关穴、膝眼穴及犊鼻穴进行离子导入,30 min/次,1次/d,三组患者均治疗4周;观察入组患者治疗前后膝关节肿胀程度及疼痛程度变化,并统计临床疗效;酶联免疫夹心吸附测定检测入组患者治疗前后膝关节液中SDF-1,CXCR4,基质金属蛋白酶-3(MMP-3)及MMP-13含量。结果:中药口服组疗效优于中药离子导入组和对照组,且复发率最低(P<0.05);与本组治疗前比较,中药口服组治疗后压痛值升高(P<0.05),中药口服组患者视觉模拟评分(VAS),膝关节肿胀评分、西安大略麦马斯特大学骨关节炎指数评分(WOMAC)及膝关节液SDF-1,CXCR4,MMP-3及MMP^-13蛋白含量均降低(P<0.05),且优于中药离子导入组和对照组(P<0.05)。结论:独活续断汤口服及离子导入治疗肝肾亏虚型KOA均有明显疗效,但口服疗效最佳,其机制可能与抑制SDF^-1/CXCR4炎症信号通路及软骨细胞降解有关。展开更多
文摘目的探讨移植基质细胞衍生因子1(SDF-1)预处理的骨髓间充质干细胞(BMSC)对大鼠颈动脉狭窄的作用。方法携带增强型绿色荧光蛋白(EGFP)的质粒转染BMSC后经静脉注入大鼠。分为颈动脉损伤模型对照组、BMSC移植组、SDF-1预处理的BMSC移植组。细胞移植术后2周,取损伤处血管组织采用免疫荧光技术检测EGFP表达明确BMSC归巢情况;移植术后4周, Evans蓝染色观察损伤内膜内皮化程度,免疫荧光技术检测损伤血管CD31的表达情况;HE染色检测损伤颈动脉新生内膜增生程度,免疫组织化学染色法检测损伤血管增殖细胞核抗原(PCNA)的表达, Western blot法检测血管内皮生长因子(VEGF)的蛋白水平。结果移植SDF-1预处理的BMSC能有效促进干细胞向损伤血管的归巢,并促进损伤血管再内皮化程度。BMSC移植后新生内膜面积、新生内膜面积/中膜面积均低于对照组, SDF-1预处理的BMSC移植组差异更为显著。移植SDF-1预处理的BMSC可显著提高损伤内膜CD31以及VEGF的水平,并抑制PCNA的表达。结论SDF-1预处理BMSC移植可通过促进BMSC归巢至损伤处并诱导细胞分化,抑制中膜平滑肌细胞增殖来减轻血管狭窄程度。
基金supported by the program (No. CX10B_421Z to Jiaxin Ye) for Postgraduate Research Innovation in Universities of Jiangsu Provincethe grants (No. 81070195) and (No. 81000055) from Chinese National Science Fund of China (all to Biao Xu)grant (No.KF200938 to Lina Kang) from Jiangsu Province
文摘This study was designed to determine the levels of early endothelial progenitor cells （EPCs）, apelin, vascu- lar endothelial growth factor （VEGF） and stromal cell-derived growth factor-1 （SDF-1） after acute myocardial infarction （AMI）, and to investigate the relationships between these cytokines and early EPCs. Early EPCs, de- fined as CD133＋, KDR＋, and CD34~ cells, were quantified by flow cytometry. The levels of early EPCs and those cytokines in AMI patients were significantly different from those with coronary artery disease or controls （P 〈 0.05）. Plasma apelin levels were inversely correlated with Gensini score and early EPCs （both P 〈 0.01）. Early EPCs, VEGF and SDF-1 showed different patterns of changes in AMI patients during the first 24 h. The trend in the change of early EPCs was proportionally correlated with that of VEGF （P 〈 0.05）. AMI patients exhibited in- creased early EPCs with remarkably decreased apelin levels and enhanced VEGF levels.
文摘目的:观察独活续断汤口服和离子导入治疗肝肾亏虚型膝骨关节炎(KOA)的临床疗效及对基质细胞衍生因子-1(SDF-1)/C-X-C趋化因子受体4型(CXCR4)信号通路的影响。方法:将150例天津中医药大学第一附属医院确诊的肝肾亏虚型KOA患者随机分为对照组、中药口服组和中药离子导入组,每组各50例,对照组给予硫酸氨基葡萄糖胶囊,口服0.5 g/次,2次/d口服,中药口服组给予独活续断汤150 m L/次,2次/d口服,中药离子导入组给予独活续断汤,于髋骨穴、膝关穴、膝眼穴及犊鼻穴进行离子导入,30 min/次,1次/d,三组患者均治疗4周;观察入组患者治疗前后膝关节肿胀程度及疼痛程度变化,并统计临床疗效;酶联免疫夹心吸附测定检测入组患者治疗前后膝关节液中SDF-1,CXCR4,基质金属蛋白酶-3(MMP-3)及MMP-13含量。结果:中药口服组疗效优于中药离子导入组和对照组,且复发率最低(P<0.05);与本组治疗前比较,中药口服组治疗后压痛值升高(P<0.05),中药口服组患者视觉模拟评分(VAS),膝关节肿胀评分、西安大略麦马斯特大学骨关节炎指数评分(WOMAC)及膝关节液SDF-1,CXCR4,MMP-3及MMP^-13蛋白含量均降低(P<0.05),且优于中药离子导入组和对照组(P<0.05)。结论:独活续断汤口服及离子导入治疗肝肾亏虚型KOA均有明显疗效,但口服疗效最佳,其机制可能与抑制SDF^-1/CXCR4炎症信号通路及软骨细胞降解有关。
