The sandwich-type lateral fl ow assays relying on dual aptamers with high sensitivity and specifi city have been broadly explored.However,it is unlikely to match a pair of specifi c aptamers that can bind a small mole...The sandwich-type lateral fl ow assays relying on dual aptamers with high sensitivity and specifi city have been broadly explored.However,it is unlikely to match a pair of specifi c aptamers that can bind a small molecular target(e.g.,cocaine)simultaneously due to the steric hindrance.In response,we herein introduced the strategy of“one divides into two”into the construction of sandwich-type lateral fl ow strip assay(LFSA).Specifi cally,we split a single cocaine-recognizing aptamer into two segments,either of which was conjugated with gold nanoparticles(AuNPs)or labeled with biotin,serving as signal probe and capture probe,respectively.Upon the presence of the target molecule,a ternary sandwich complex comprised of the two halves of the aptamer and the target formed.Such sandwich-type LFSA exhibited an excellent nonlinear logarithmic response in the range from 10μmol/L to 5 mmol/L with R^(2)=0.9994.The sensitive on-site detection of cocaine in artifi cial biological samples including urine and sweat was achieved within 15 min,with the visual limit of detection as low as 50μmol/L for urine and 200μmol/L for sweat,and the recovery rates of 83.6-107.4%.展开更多
The elastostatic solutions of semi-infinite orthotropic cantilevered strips with traction free edges and loading at infinity are governed by the differential equationφ,■+(2+δ_(0))φ,■+φ,■=0 withδ_(0)>-4 with...The elastostatic solutions of semi-infinite orthotropic cantilevered strips with traction free edges and loading at infinity are governed by the differential equationφ,■+(2+δ_(0))φ,■+φ,■=0 withδ_(0)>-4 with Based on the work of[10]forδ_(0)>0 case,.this paper completes the caseδ_(0)=0 for isotropic materials and the case 0>δ_(0)>-4 for orthotropic materials.The solutions of the above problems have important application in the properly formulated boundary conditions of plate theories for prescribed displacement edge data.展开更多
基金supported by the National Natural Science Foundation of China(22090050,22122410,21874121)the National Key Research and Development Program of China(2018YFE0206900)+1 种基金Hubei Provincial Natural Science Foundation of China(2020CFA037)Zhejiang Provincial Natural Science Foundation of China(LD21B050001)
文摘The sandwich-type lateral fl ow assays relying on dual aptamers with high sensitivity and specifi city have been broadly explored.However,it is unlikely to match a pair of specifi c aptamers that can bind a small molecular target(e.g.,cocaine)simultaneously due to the steric hindrance.In response,we herein introduced the strategy of“one divides into two”into the construction of sandwich-type lateral fl ow strip assay(LFSA).Specifi cally,we split a single cocaine-recognizing aptamer into two segments,either of which was conjugated with gold nanoparticles(AuNPs)or labeled with biotin,serving as signal probe and capture probe,respectively.Upon the presence of the target molecule,a ternary sandwich complex comprised of the two halves of the aptamer and the target formed.Such sandwich-type LFSA exhibited an excellent nonlinear logarithmic response in the range from 10μmol/L to 5 mmol/L with R^(2)=0.9994.The sensitive on-site detection of cocaine in artifi cial biological samples including urine and sweat was achieved within 15 min,with the visual limit of detection as low as 50μmol/L for urine and 200μmol/L for sweat,and the recovery rates of 83.6-107.4%.
文摘The elastostatic solutions of semi-infinite orthotropic cantilevered strips with traction free edges and loading at infinity are governed by the differential equationφ,■+(2+δ_(0))φ,■+φ,■=0 withδ_(0)>-4 with Based on the work of[10]forδ_(0)>0 case,.this paper completes the caseδ_(0)=0 for isotropic materials and the case 0>δ_(0)>-4 for orthotropic materials.The solutions of the above problems have important application in the properly formulated boundary conditions of plate theories for prescribed displacement edge data.
