Insect symbionts Wolbachia used for pest population control has focused on vector pest species and agricultural insects while rare reports in Chinese stored-product insect samples.In this paper,we surveyed the prevale...Insect symbionts Wolbachia used for pest population control has focused on vector pest species and agricultural insects while rare reports in Chinese stored-product insect samples.In this paper,we surveyed the prevalence of Wolbachia using a PCR detection method in ten Tenebrionidae stored-product insects.Subsequently,the spatiotemporal Wolbachia infection dynamics in Tribolium confusum and Wolbachia elimination patterns using tetracycline treatment were investigated in detail by TaqMan®probe real-time quantitative PCR,and host reproductive fitness parameters were compared.T.confusum was the only Wolbachia infected species in all the surveyed species.Wolbachia infection density consistently increased with the development of T.confusumand plateaued at 3.7×107 wsp copies per individual insect at the young adult stage.Wolbachia densities in females were higher than that in males with a significant difference at the pupae stage and varied among different tissues and organs.Uninfected female beetles were completely incapable of producing mature progenies when crossed with Wolbachia infected males.Embryogenesis and egg hatch rate were specifically inhibited after Wolbachia elimination,while other traits,including the number of eggs,pupation rate and sex ratio,remained unaffected by tetracycline treatment.Our results showthat the TaqMan®probe qPCR is a reliable detection method for quantifying the density of Wolbachia as compared to qualitative detection of wsp gene by PCR and relatively quantified by real-time qPCR.The fitness results indicated that Wolbachia infection was not an obligate symbiont and benefited the host confused flour beetle.展开更多
Long-grain rice cultivars Cocodrie, Wells, and XP 723 grown in three locations （Hazen, MO; Essex and Newport, AR, USA）, and medium-grain rice cultivars Bengal and XP 713 grown in two locations （Jonesboro and Lodge ...Long-grain rice cultivars Cocodrie, Wells, and XP 723 grown in three locations （Hazen, MO; Essex and Newport, AR, USA）, and medium-grain rice cultivars Bengal and XP 713 grown in two locations （Jonesboro and Lodge Corner, AR, USA）, were harvested and assayed for susceptibility to Rhyzopertha dominica （F.） （Coleoptera： Bostrichidae）, the lesser grain borer, and Sitophilus oryzae （L.） （Coleoptera： Curculionidae）, the rice weevil, on rice held at 27℃, 57% and 75% relative humidity （RH）. Separate samples from the same harvest lots were also analyzed for the physical characteristics of brown rice yield, percentage whole kernels and kernel thickness. Progeny production and feeding damage of R. dominica were significantly different among long-grain cultivars within two of the three locations （P 〈 0.05）, but not for location or RH （P ≥ 0.05）, while progeny production of S. oryzae was different among cultivars, location, and RH （P 〈 0.05）. On medium-grain rice, both cultivar and location were significant for progeny production of R. dominica, but not RH, while cultivar and RH were significant for progeny production of S. oryzae, but not location. On both rice types, feeding damage of R. dominica followed the same trends and was always strongly positively correlated with progeny production （P 〈 0.05）, but for S. oryzae there were several instances in which progeny production was not correlated with feeding damage （P ≥ 0.05）. Physical characteristics of both rice types were statistically significant （P 〈 0.01） but actual numerical differences were extremely small, and were generally not correlated with progeny production of either species. Results indicate that the location in which a particular rice cultivar is grown, along with its characteristics, could affect susceptibility of the rice to R. dominica and S. oryzae.展开更多
基金supported by National Natural Science Fund Project (No.31601890)the Fundamental Research Funds for the Henan Provincial Colleges and Universities in Henan University of Technology (No.2016XTCX01)
文摘Insect symbionts Wolbachia used for pest population control has focused on vector pest species and agricultural insects while rare reports in Chinese stored-product insect samples.In this paper,we surveyed the prevalence of Wolbachia using a PCR detection method in ten Tenebrionidae stored-product insects.Subsequently,the spatiotemporal Wolbachia infection dynamics in Tribolium confusum and Wolbachia elimination patterns using tetracycline treatment were investigated in detail by TaqMan®probe real-time quantitative PCR,and host reproductive fitness parameters were compared.T.confusum was the only Wolbachia infected species in all the surveyed species.Wolbachia infection density consistently increased with the development of T.confusumand plateaued at 3.7×107 wsp copies per individual insect at the young adult stage.Wolbachia densities in females were higher than that in males with a significant difference at the pupae stage and varied among different tissues and organs.Uninfected female beetles were completely incapable of producing mature progenies when crossed with Wolbachia infected males.Embryogenesis and egg hatch rate were specifically inhibited after Wolbachia elimination,while other traits,including the number of eggs,pupation rate and sex ratio,remained unaffected by tetracycline treatment.Our results showthat the TaqMan®probe qPCR is a reliable detection method for quantifying the density of Wolbachia as compared to qualitative detection of wsp gene by PCR and relatively quantified by real-time qPCR.The fitness results indicated that Wolbachia infection was not an obligate symbiont and benefited the host confused flour beetle.
文摘Long-grain rice cultivars Cocodrie, Wells, and XP 723 grown in three locations （Hazen, MO; Essex and Newport, AR, USA）, and medium-grain rice cultivars Bengal and XP 713 grown in two locations （Jonesboro and Lodge Corner, AR, USA）, were harvested and assayed for susceptibility to Rhyzopertha dominica （F.） （Coleoptera： Bostrichidae）, the lesser grain borer, and Sitophilus oryzae （L.） （Coleoptera： Curculionidae）, the rice weevil, on rice held at 27℃, 57% and 75% relative humidity （RH）. Separate samples from the same harvest lots were also analyzed for the physical characteristics of brown rice yield, percentage whole kernels and kernel thickness. Progeny production and feeding damage of R. dominica were significantly different among long-grain cultivars within two of the three locations （P 〈 0.05）, but not for location or RH （P ≥ 0.05）, while progeny production of S. oryzae was different among cultivars, location, and RH （P 〈 0.05）. On medium-grain rice, both cultivar and location were significant for progeny production of R. dominica, but not RH, while cultivar and RH were significant for progeny production of S. oryzae, but not location. On both rice types, feeding damage of R. dominica followed the same trends and was always strongly positively correlated with progeny production （P 〈 0.05）, but for S. oryzae there were several instances in which progeny production was not correlated with feeding damage （P ≥ 0.05）. Physical characteristics of both rice types were statistically significant （P 〈 0.01） but actual numerical differences were extremely small, and were generally not correlated with progeny production of either species. Results indicate that the location in which a particular rice cultivar is grown, along with its characteristics, could affect susceptibility of the rice to R. dominica and S. oryzae.
