AIM:To reveal the functions of micro RNAs(mi RNAs) with respect to hepatic stellate cells(HSCs) in response to portal hypertension.METHODS:Primary rat HSCs were exposed to static water pressure(10 mm Hg,1 h) and the p...AIM:To reveal the functions of micro RNAs(mi RNAs) with respect to hepatic stellate cells(HSCs) in response to portal hypertension.METHODS:Primary rat HSCs were exposed to static water pressure(10 mm Hg,1 h) and the pressureinduced mi RNA expression profile was detected by next-generation sequencing. Quantitative real-time polymerase chain reaction was used to verify the expression of mi RNAs. A potential target of Mi R-9a-5p was measured by a luciferase reporter assay and Western blot. CCK-8 assay and Transwell assay were used to detect the proliferation and migration of HSCs under pressure.RESULTS:According to the profile,the expression of mi R-9a-5p was further confirmed to be significantly increased after pressure overload in HSCs(3.70 ± 0.61 vs 0.97 ± 0.15,P = 0.0226),which resulted in the proliferation,migration and activation of HSCs. In vivo,the up-regulation of mi R-9a-5p(2.09 ± 0.91 vs 4.27 ± 1.74,P = 0.0025) and the down-regulation of Sirt1(2.41 ± 0.51 vs 1.13 ± 0.11,P = 0.0006) were observed in rat fibrotic liver with portal hypertension. Sirt1 was a potential target gene of mi R-9a-5p. Through restoringthe expression of Sirt1 in mi R-9a-5p transfected HSCs on pressure overload,we found that overexpression of Sirt1 could partially abrogate the mi R-9a-5p mediated suppression of the proliferation,migration and activation of HSCs. CONCLUSION:Our results suggest that during liver fibrosis,portal hypertension may induce the proliferation,migration and activation of HSCs through the up-regulation of mi R-9a-5p,which targets Sirt1.展开更多
Abstract Cities based on coal resources have increasingly important social and economic roles in China. Their strategies for sustainable development, however, urgently need to be improved, which represents a huge chal...Abstract Cities based on coal resources have increasingly important social and economic roles in China. Their strategies for sustainable development, however, urgently need to be improved, which represents a huge challenge. Most observers believe that the continued progress of these cities relies on the optimization of scientific adaptive management in which social, economic, and ecological factors are incorporated. A systems perspective that combines policies, management priorities, and long-term policy impacts needs to be applied. To date, however, such an approach has not been adopted, which means it is difficult to implement adaptive management at the regional scale. In this study, we used various situations to develop a multiple adaptive scenario system dynamics model. We then simulated a range of policy scenarios, with Ordos in the Inner Mongolia Autonomous Region as a case study. Simulation results showed that the current strategy is not sustainable and predicted that the system would exceed the environmental capacity, with risks of resource exhaustion and urban decline in 2025-2035. Five critical policy variables, including the urban population carrying capacity, rates of water consumption and water recycling, and expansion of urban land cover, were identified during sensitivity analysis. We developed and compared six socio-economic scenarios. The urban area, represented by the urban population density, seemed to transition through five different stages, namely natural growth, rapid growth, stable oscillation, fading, and rebalancing. Our scenarios suggested that different policies had different roles through each stage. The water use efficiency management policy had a comprehensive far-reaching influence on the system behavior; land urbanization management functions dominated at the start, and population capacity management was a major control in the mid-term. Our results showed that the water recycling policy and the urban population carrying capacity were extremely important, and both should be reinfor展开更多
Saussurea plants have a long history of being used for disease prevention and control in Tibetan medicine, and the species have gradually changed along with the development of history. Saussurea is mainly effective in...Saussurea plants have a long history of being used for disease prevention and control in Tibetan medicine, and the species have gradually changed along with the development of history. Saussurea is mainly effective in wind-expelling, blood circulation, spasmolysis, pain relief, heat-clearing and detoxification. Characteristics, major chemical constituents and pharmacological studies of Saussurea stella Maxim. were summarized in this review based on the utilization of Saussurea medicinal plants in Tibetan areas, with the aim to provide adequate theoretical bases and references for exploring the further medicinal value and development and utilization of S. stella .展开更多
基金Supported by National Natural Science Foundation of China,No.11272342/A0205
文摘AIM:To reveal the functions of micro RNAs(mi RNAs) with respect to hepatic stellate cells(HSCs) in response to portal hypertension.METHODS:Primary rat HSCs were exposed to static water pressure(10 mm Hg,1 h) and the pressureinduced mi RNA expression profile was detected by next-generation sequencing. Quantitative real-time polymerase chain reaction was used to verify the expression of mi RNAs. A potential target of Mi R-9a-5p was measured by a luciferase reporter assay and Western blot. CCK-8 assay and Transwell assay were used to detect the proliferation and migration of HSCs under pressure.RESULTS:According to the profile,the expression of mi R-9a-5p was further confirmed to be significantly increased after pressure overload in HSCs(3.70 ± 0.61 vs 0.97 ± 0.15,P = 0.0226),which resulted in the proliferation,migration and activation of HSCs. In vivo,the up-regulation of mi R-9a-5p(2.09 ± 0.91 vs 4.27 ± 1.74,P = 0.0025) and the down-regulation of Sirt1(2.41 ± 0.51 vs 1.13 ± 0.11,P = 0.0006) were observed in rat fibrotic liver with portal hypertension. Sirt1 was a potential target gene of mi R-9a-5p. Through restoringthe expression of Sirt1 in mi R-9a-5p transfected HSCs on pressure overload,we found that overexpression of Sirt1 could partially abrogate the mi R-9a-5p mediated suppression of the proliferation,migration and activation of HSCs. CONCLUSION:Our results suggest that during liver fibrosis,portal hypertension may induce the proliferation,migration and activation of HSCs through the up-regulation of mi R-9a-5p,which targets Sirt1.
基金supported by the National Natural Science Foundation of China(Grant Nos.41590845&41601096)the China Postdoctoral Science Foundation(Grant No.2015M581160)
文摘Abstract Cities based on coal resources have increasingly important social and economic roles in China. Their strategies for sustainable development, however, urgently need to be improved, which represents a huge challenge. Most observers believe that the continued progress of these cities relies on the optimization of scientific adaptive management in which social, economic, and ecological factors are incorporated. A systems perspective that combines policies, management priorities, and long-term policy impacts needs to be applied. To date, however, such an approach has not been adopted, which means it is difficult to implement adaptive management at the regional scale. In this study, we used various situations to develop a multiple adaptive scenario system dynamics model. We then simulated a range of policy scenarios, with Ordos in the Inner Mongolia Autonomous Region as a case study. Simulation results showed that the current strategy is not sustainable and predicted that the system would exceed the environmental capacity, with risks of resource exhaustion and urban decline in 2025-2035. Five critical policy variables, including the urban population carrying capacity, rates of water consumption and water recycling, and expansion of urban land cover, were identified during sensitivity analysis. We developed and compared six socio-economic scenarios. The urban area, represented by the urban population density, seemed to transition through five different stages, namely natural growth, rapid growth, stable oscillation, fading, and rebalancing. Our scenarios suggested that different policies had different roles through each stage. The water use efficiency management policy had a comprehensive far-reaching influence on the system behavior; land urbanization management functions dominated at the start, and population capacity management was a major control in the mid-term. Our results showed that the water recycling policy and the urban population carrying capacity were extremely important, and both should be reinfor
基金Supported by the Fundamental Research Funds for the Central Universities:the Young Teachers Fund of Southwest Minzu University(2017NZYQN38)the Planning Project for Science and Technology Training of Sichuan Province(2016KZ0007)
文摘Saussurea plants have a long history of being used for disease prevention and control in Tibetan medicine, and the species have gradually changed along with the development of history. Saussurea is mainly effective in wind-expelling, blood circulation, spasmolysis, pain relief, heat-clearing and detoxification. Characteristics, major chemical constituents and pharmacological studies of Saussurea stella Maxim. were summarized in this review based on the utilization of Saussurea medicinal plants in Tibetan areas, with the aim to provide adequate theoretical bases and references for exploring the further medicinal value and development and utilization of S. stella .
