Viral-mediated gene transfer of thymidine kinase ofherpes simplex virus (HSV-tk) has been used to confercytotoxic sensitivity to ganciclovir (GCV) in a variety oftnmor cells. HSV-tk converts GCV into a phosphorylatedc...Viral-mediated gene transfer of thymidine kinase ofherpes simplex virus (HSV-tk) has been used to confercytotoxic sensitivity to ganciclovir (GCV) in a variety oftnmor cells. HSV-tk converts GCV into a phosphorylatedcompound which is toxic for dividing cells by blockingDNA synthesis. Our previous study has shown展开更多
Herpes simplex virus （HSV）, a member of the Herpesviridae family, is a significant human pathogen that results in mucocutaneous lesions in the oral cavity or genital infections. Acyclovir （ACV） and related nucleos...Herpes simplex virus （HSV）, a member of the Herpesviridae family, is a significant human pathogen that results in mucocutaneous lesions in the oral cavity or genital infections. Acyclovir （ACV） and related nucleoside analogues can successfully treat HSV infections, but the emergence of drug resistance to ACV has created a barrier for the treatment of HSV infections, especially in immunocompromised patients. There is an urgent need to explore new and effective tactics to circumvent drug resistance to HSV. This review summarises the current strategies in the development of new targets （the DNA helicase/primase （H/P） complex）, new types of molecules （nature products） and new antiviral mechanisms （lethal mutagenesis of Janus-type nucleosides） to fight the drug resistance of HSV.展开更多
Herpes simplex virus type 1(HSV-1),a neurotropic herpes virus,is able to establish a lifelong latent infection in the human host.Following primary replication in mucosal epithelial cells,the virus can enter sensory ne...Herpes simplex virus type 1(HSV-1),a neurotropic herpes virus,is able to establish a lifelong latent infection in the human host.Following primary replication in mucosal epithelial cells,the virus can enter sensory neurons innervating peripheral tissues via nerve termini.The viral genome is then transported to the nucleus where it can be maintained without producing infectious progeny,and thus latency is established in the cell.Yin-Yang balance is an essential concept in traditional Chinese medicine(TCM)theory.Yin represents stable and inhibitory factors,and Yang represents the active and aggressive factors.When the organism is exposed to stress,especially psychological stress caused by emotional stimulation,the Yin-Yang balance is disturbed and the virus can re-engage in productive replication,resulting in recurrent diseases.Therefore,a better understanding of the stress-induced susceptibility to HSV-1 primary infection and reactivation is needed and will provide helpful insights into the effective control and treatment of HSV-1.Here we reviewed the recent advances in the studies of HSV-1 susceptibility,latency and reactivation.We included mechanisms involved in primary infection and the regulation of latency and described how stress-induced changes increase the susceptibility to primary and recurrent infections.展开更多
抑制风电场次同步振荡(sub-synchronous oscillations,SSO)的晶闸管可控串联补偿装置(thyristor controlled series compensation,TCSC)参数优化问题是强非线性参数优化问题,很难用解析方法求解。文中将单纯形算法(Simplex)与电磁暂态...抑制风电场次同步振荡(sub-synchronous oscillations,SSO)的晶闸管可控串联补偿装置(thyristor controlled series compensation,TCSC)参数优化问题是强非线性参数优化问题,很难用解析方法求解。文中将单纯形算法(Simplex)与电磁暂态仿真程序结合的非线性参数优化方法引入到抑制SSO的TCSC参数的优化。该算法使用电磁暂态程序的仿真结果计算优化目标函数值,Simplex算法应用电磁暂态程序计算的优化目标函数结果智能地指导TCSC参数寻优方向,快速可靠地寻到最优参数,缩短仿真耗时。最后,针对风电不确定性,初步分析了风速变化对参数优化结果的影响,根据所采用的单机等值系统仿真结果分析,风速变化对TCSC参数优化结果的影响并不显著。展开更多
文摘Viral-mediated gene transfer of thymidine kinase ofherpes simplex virus (HSV-tk) has been used to confercytotoxic sensitivity to ganciclovir (GCV) in a variety oftnmor cells. HSV-tk converts GCV into a phosphorylatedcompound which is toxic for dividing cells by blockingDNA synthesis. Our previous study has shown
基金the National Natural Science Foundations of China(document no.:81321002,81500860,81300888)a grant from 111 Project of Ministry of Education,China,for fi nancial support
文摘Herpes simplex virus （HSV）, a member of the Herpesviridae family, is a significant human pathogen that results in mucocutaneous lesions in the oral cavity or genital infections. Acyclovir （ACV） and related nucleoside analogues can successfully treat HSV infections, but the emergence of drug resistance to ACV has created a barrier for the treatment of HSV infections, especially in immunocompromised patients. There is an urgent need to explore new and effective tactics to circumvent drug resistance to HSV. This review summarises the current strategies in the development of new targets （the DNA helicase/primase （H/P） complex）, new types of molecules （nature products） and new antiviral mechanisms （lethal mutagenesis of Janus-type nucleosides） to fight the drug resistance of HSV.
基金supported,in part,by the National Key Research and Development Program of China,China(grant number2017YFC1700404)Natural Science Foundation of China,China(grant numbers 81622050,81573675,81673709,81560661 and81873209)+3 种基金the Young Top-notch Talent Support Program of Guangdong Province,China(grant numbers 2014TQ01R229 and2016TQ03R586)Guangdong Province Ocean and Fisheries Bureau-Key Technology Research and Development Program,China(grant number A201701A02)Guangdong Science and Technology Foundation for Distinguished Young Scholars,China(grant number 2017A030306004)the Science and Technology Program of Guangzhou,China(grant numbers 201604046016 and201610010182).
文摘Herpes simplex virus type 1(HSV-1),a neurotropic herpes virus,is able to establish a lifelong latent infection in the human host.Following primary replication in mucosal epithelial cells,the virus can enter sensory neurons innervating peripheral tissues via nerve termini.The viral genome is then transported to the nucleus where it can be maintained without producing infectious progeny,and thus latency is established in the cell.Yin-Yang balance is an essential concept in traditional Chinese medicine(TCM)theory.Yin represents stable and inhibitory factors,and Yang represents the active and aggressive factors.When the organism is exposed to stress,especially psychological stress caused by emotional stimulation,the Yin-Yang balance is disturbed and the virus can re-engage in productive replication,resulting in recurrent diseases.Therefore,a better understanding of the stress-induced susceptibility to HSV-1 primary infection and reactivation is needed and will provide helpful insights into the effective control and treatment of HSV-1.Here we reviewed the recent advances in the studies of HSV-1 susceptibility,latency and reactivation.We included mechanisms involved in primary infection and the regulation of latency and described how stress-induced changes increase the susceptibility to primary and recurrent infections.
文摘抑制风电场次同步振荡(sub-synchronous oscillations,SSO)的晶闸管可控串联补偿装置(thyristor controlled series compensation,TCSC)参数优化问题是强非线性参数优化问题,很难用解析方法求解。文中将单纯形算法(Simplex)与电磁暂态仿真程序结合的非线性参数优化方法引入到抑制SSO的TCSC参数的优化。该算法使用电磁暂态程序的仿真结果计算优化目标函数值,Simplex算法应用电磁暂态程序计算的优化目标函数结果智能地指导TCSC参数寻优方向,快速可靠地寻到最优参数,缩短仿真耗时。最后,针对风电不确定性,初步分析了风速变化对参数优化结果的影响,根据所采用的单机等值系统仿真结果分析,风速变化对TCSC参数优化结果的影响并不显著。
