Objective To characterize the meningococcal strains isolated from cases and close contacts with meningococcal disease associated with an outbreak in a jail in May 2010 by investigating the national distribution of hyp...Objective To characterize the meningococcal strains isolated from cases and close contacts with meningococcal disease associated with an outbreak in a jail in May 2010 by investigating the national distribution of hyperinvasive ST-4821 serogroup C clone associated with this outbreak. Methods The cases were described based on the clinical symptoms and laboratory results. Pharyngeal swabs were cultured for N. meningitidis from men in the jail. Meningococcal isolates were identified by serogrouping, pulsed-field gel electrophoresis （PFGE） and multilocus sequence typing （MLST）, respectively. Four hundred and sixteen serogroup C N. meningitidis strains were collected from 27 provinces between 2003 and 2010 for a nationwide survey and analyzed by PFGE and MLST. Results Three persons in a jail system were infected with invasive N. meningitidis serogroup C. All isolates tested had matching PFGE patterns and belonged to the multilocus sequence type （ST） 4821 clonal complex. All 47 N. meningitidis strains were identified from the pharyngeal swabs of 166 peoples in the jail, and 26 of them belonged to ST-4821 serogroup C clone, and 90.14% （375/416） serogroup C strains identified in the nationwide survey belonged to the ST-4821 complex. The ST-4821 serogroup C clone was spread nationwide, distributed in 24 provinces, especially in eastern provinces between 2003 and 2010. Conclusion Endemic transmission and carriage rate of ST-4821 serogroup C clone are high in this jail system. The ST-4821 serogroup C clone is spreading in China and nationwide distributed despite the existence of some effective vaccines.展开更多
目的利用脑膜炎奈瑟菌基因组中可变数目串联重复序列(VNTR)特征,对中国C群脑膜炎奈瑟菌菌株进行基因分型。方法中国C群脑膜炎奈瑟菌菌株109株,选择脑膜炎奈瑟菌DNA中4个VNTR位点,PCR扩增含有串联重复序列的DNA片段,选择每一个VNTR位点...目的利用脑膜炎奈瑟菌基因组中可变数目串联重复序列(VNTR)特征,对中国C群脑膜炎奈瑟菌菌株进行基因分型。方法中国C群脑膜炎奈瑟菌菌株109株,选择脑膜炎奈瑟菌DNA中4个VNTR位点,PCR扩增含有串联重复序列的DNA片段,选择每一个VNTR位点有差别的PCR产物进行测序,序列比对,测算串联重复序列的拷贝数。Bio-Rad Gel DocTM XR凝胶成像分析系统计算PCR产物DNA片段的碱基含量,换算成串联重复数;对109株菌株4个位点的串联重复序列拷贝数进行聚类分析,依据聚类分析结果进行基因分型,并将VNTR基因分型结果与脉冲场凝胶电泳基因分型(PFGE)结果进行比较。结果109株C群脑膜炎奈瑟菌菌株分为22个VNTR基因型,同一暴发来源的菌株具有相同VNTR特征;VNTR基因分型方法与PFGE基因分型具有相关关系。结论应用VNTR技术可以对中国C群脑膜炎奈瑟菌进行基因分型和分子流行病学方面的研究,VNTR基因分型可较好地应用于追溯流行性脑脊髓膜炎暴发传染源。展开更多
基金supported by Research Fund from Ministry of Health and Ministry of Science and Technology of the People’s Republic of China,2013ZX10004221,2012ZX10004215,and 2011CB504900
文摘Objective To characterize the meningococcal strains isolated from cases and close contacts with meningococcal disease associated with an outbreak in a jail in May 2010 by investigating the national distribution of hyperinvasive ST-4821 serogroup C clone associated with this outbreak. Methods The cases were described based on the clinical symptoms and laboratory results. Pharyngeal swabs were cultured for N. meningitidis from men in the jail. Meningococcal isolates were identified by serogrouping, pulsed-field gel electrophoresis （PFGE） and multilocus sequence typing （MLST）, respectively. Four hundred and sixteen serogroup C N. meningitidis strains were collected from 27 provinces between 2003 and 2010 for a nationwide survey and analyzed by PFGE and MLST. Results Three persons in a jail system were infected with invasive N. meningitidis serogroup C. All isolates tested had matching PFGE patterns and belonged to the multilocus sequence type （ST） 4821 clonal complex. All 47 N. meningitidis strains were identified from the pharyngeal swabs of 166 peoples in the jail, and 26 of them belonged to ST-4821 serogroup C clone, and 90.14% （375/416） serogroup C strains identified in the nationwide survey belonged to the ST-4821 complex. The ST-4821 serogroup C clone was spread nationwide, distributed in 24 provinces, especially in eastern provinces between 2003 and 2010. Conclusion Endemic transmission and carriage rate of ST-4821 serogroup C clone are high in this jail system. The ST-4821 serogroup C clone is spreading in China and nationwide distributed despite the existence of some effective vaccines.
文摘目的利用脑膜炎奈瑟菌基因组中可变数目串联重复序列(VNTR)特征,对中国C群脑膜炎奈瑟菌菌株进行基因分型。方法中国C群脑膜炎奈瑟菌菌株109株,选择脑膜炎奈瑟菌DNA中4个VNTR位点,PCR扩增含有串联重复序列的DNA片段,选择每一个VNTR位点有差别的PCR产物进行测序,序列比对,测算串联重复序列的拷贝数。Bio-Rad Gel DocTM XR凝胶成像分析系统计算PCR产物DNA片段的碱基含量,换算成串联重复数;对109株菌株4个位点的串联重复序列拷贝数进行聚类分析,依据聚类分析结果进行基因分型,并将VNTR基因分型结果与脉冲场凝胶电泳基因分型(PFGE)结果进行比较。结果109株C群脑膜炎奈瑟菌菌株分为22个VNTR基因型,同一暴发来源的菌株具有相同VNTR特征;VNTR基因分型方法与PFGE基因分型具有相关关系。结论应用VNTR技术可以对中国C群脑膜炎奈瑟菌进行基因分型和分子流行病学方面的研究,VNTR基因分型可较好地应用于追溯流行性脑脊髓膜炎暴发传染源。
