The maximum level of organophosphate pesticide residues in rice is 0.1 mg/kg and 0.5 mg/kg in vegetables. The control of pesticide residues in agricultural products required a method of analysis quickly and accurately...The maximum level of organophosphate pesticide residues in rice is 0.1 mg/kg and 0.5 mg/kg in vegetables. The control of pesticide residues in agricultural products required a method of analysis quickly and accurately. The research developed a biosensor for the detection of organophosphate pesticide residues in agricultural products. The research studied immobilized organophosphate hydrolase (OPH) mass and characterization of biosensor. The solution conductivity measurement in the conductivity cell consists of a 1 × 5 mm2 pair of electrodes screen printed carbon electrode (SPCE). The instrument is a converted local conductometer. From the results of study concluded that the optimum performance of the biosensor was obtained from the 105 μg OPH, at pH 8.5 with a response time of 45 seconds. In that condition the sensitivity of biosensor is 28.04 μS/ppm and 0.18 ppm detection limit and the maximum concentration of pesticide which can be measured is 1 ppm. Biosensors have been applied to measure pesticide residues in some vegetable samples.展开更多
目的以甲型H1N1流感病毒为检测对象,建立一种基于电化学传感技术的流感病毒的检测方法,用于流感病毒的现场快速检测和临床初步筛查。方法将甲型H1N1流感病毒的捕获抗体修饰于丝网印刷电极（SPCE）表面,与抗原结合后,通过辣根过氧化物酶...目的以甲型H1N1流感病毒为检测对象,建立一种基于电化学传感技术的流感病毒的检测方法,用于流感病毒的现场快速检测和临床初步筛查。方法将甲型H1N1流感病毒的捕获抗体修饰于丝网印刷电极（SPCE）表面,与抗原结合后,通过辣根过氧化物酶（HRP）标记的检测抗体与其形成免疫夹心复合物,进行信号放大并测定其电化学参数变化以检测目标抗原含量。结果所建立的电化学免疫传感检测器能特异识别甲型H1N1流感病毒,与甲型H3N2和甲型H7N9的HA蛋白、乙型流感病毒、腺病毒和EV71病毒等无交叉反应,检测线性范围为4～64 HA Unit,检测下限为0.41 HA Unit,批内重复性结果 RSD值均〈10%,回收率均＞90%,整个检测过程可在3 h内完成。结论该电化学免疫传感器可实现对甲型H1N1流感病毒的准确和快速检测,且检测设备易携带,有望用于流感病毒的现场快速筛查。展开更多
文摘The maximum level of organophosphate pesticide residues in rice is 0.1 mg/kg and 0.5 mg/kg in vegetables. The control of pesticide residues in agricultural products required a method of analysis quickly and accurately. The research developed a biosensor for the detection of organophosphate pesticide residues in agricultural products. The research studied immobilized organophosphate hydrolase (OPH) mass and characterization of biosensor. The solution conductivity measurement in the conductivity cell consists of a 1 × 5 mm2 pair of electrodes screen printed carbon electrode (SPCE). The instrument is a converted local conductometer. From the results of study concluded that the optimum performance of the biosensor was obtained from the 105 μg OPH, at pH 8.5 with a response time of 45 seconds. In that condition the sensitivity of biosensor is 28.04 μS/ppm and 0.18 ppm detection limit and the maximum concentration of pesticide which can be measured is 1 ppm. Biosensors have been applied to measure pesticide residues in some vegetable samples.
文摘目的以甲型H1N1流感病毒为检测对象,建立一种基于电化学传感技术的流感病毒的检测方法,用于流感病毒的现场快速检测和临床初步筛查。方法将甲型H1N1流感病毒的捕获抗体修饰于丝网印刷电极（SPCE）表面,与抗原结合后,通过辣根过氧化物酶（HRP）标记的检测抗体与其形成免疫夹心复合物,进行信号放大并测定其电化学参数变化以检测目标抗原含量。结果所建立的电化学免疫传感检测器能特异识别甲型H1N1流感病毒,与甲型H3N2和甲型H7N9的HA蛋白、乙型流感病毒、腺病毒和EV71病毒等无交叉反应,检测线性范围为4～64 HA Unit,检测下限为0.41 HA Unit,批内重复性结果 RSD值均〈10%,回收率均＞90%,整个检测过程可在3 h内完成。结论该电化学免疫传感器可实现对甲型H1N1流感病毒的准确和快速检测,且检测设备易携带,有望用于流感病毒的现场快速筛查。
