[Objectives]To explore the molecular mechanism of Sanzi Yangqin Decoction in the treatment of bronchial asthma based on network pharmacology and molecular docking.[Methods]The components of Fructus Perillae,Semen Raph...[Objectives]To explore the molecular mechanism of Sanzi Yangqin Decoction in the treatment of bronchial asthma based on network pharmacology and molecular docking.[Methods]The components of Fructus Perillae,Semen Raphani and Semen Sinapis three traditional Chinese medicine-related components and targets of Feiduqing Sanzi Yangqin Decoction were obtained using Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),and the targets of bronchial asthma were obtained using Genecards and OMIM databases.Sanzi Yangqin Decoction"drug-active ingredient-target-disease"network was established with the aid of Cytoscape 3.7.2 software and network topology analysis was carried out.The gene ontology(GO)function enrichment analysis and the KEGG pathway enrichment analysis were performed by DAVID.The top 3 components and targets in the network topology analysis were respectively molecularly docked.[Results]Through network analysis,4 key active components were obtained,mainly luteolin,arachidonic acid,β-carotene,etc.;5 key targets,mainly NCOA2,PGR,PTGS2,etc.Through GO analysis,523 items(P<0.05)were obtained,including 396 items in biological process(BP),53 items in cell composition(CC),and 74 items in molecular function(MF).KEGG analysis generated 144 signal pathways(P<0.05),involving PI3K-Akt signal pathway,human cytomegalovirus infection,Kaposi's sarcoma-associated herpes virus infection,proteoglycans in cancer,prostate cancer,etc.The results of molecular docking showed that core active compounds such as luteolin andβ-carotene in Sanzi Yangqin Decoction had good affinity with NCOA2,PGR,PTGS2 and other target genes,which were similar to clinically recommended chemical drugs.[Conclusions]The active compounds luteolin and carotene in Sanzi Yangqin Decoction may mainly bind to targets such as NCOA2,PGR,PTGS2,and regulate multiple signaling pathways such as PI3K-Akt to play a role in treating bronchial asthma.It is intended to provide new ideas for the clinical application and research of Sanzi Yangqi展开更多
目的:观察三子养亲汤加味对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)稳定期患者的临床疗效、活动耐力及气道黏液高分泌的影响。方法:90例COPD稳定期患者随机分为对照组和观察组各45例。对照组患者给予常规西医治...目的:观察三子养亲汤加味对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)稳定期患者的临床疗效、活动耐力及气道黏液高分泌的影响。方法:90例COPD稳定期患者随机分为对照组和观察组各45例。对照组患者给予常规西医治疗,包括氨茶碱缓释片和沙丁胺醇气雾剂等;观察组在对照组治疗基础上联用三子养亲汤加味治疗,2周为1个疗程,连续治疗3个疗程。治疗后,比较两组患者活动耐力、中医证候评分、肺功能[用力肺活量(forced vital capacity,FVC)、第1秒用力呼气容积(the first second force expiratory volume,FEV1)和FEV1%]、血清炎性因子[白细胞介素-8(interleukin-8,IL-8)、IL-10和中性粒细胞弹性蛋白酶(neutrophil elastase,NE)]的含量,并评价疗效。结果:治疗后,两组患者FEV1和FEV1%均升高,其中观察组FEV1高于对照组,差异有统计学意义(P<0.05);治疗后,两组患者6分钟步行距离(6-minutes walk desease,6-MWD)均增加,观察组6-MWD高于对照组(P<0.05);治疗后,两组患者IL-8和IL-10均下降,且观察组低于对照组(P<0.05);观察组NE明显下降,且低于对照组(P<0.05);治疗后,两组患者中医证候评分均降低,且观察组评分低于对照组(P<0.05);观察组有效率为93.33%,对照组有效率为77.78%,两组有效率比较,差异有统计学意义(P<0.05)。结论:三子养亲汤加味治疗稳定期COPD疗效显著,可以明显提高患者活动耐力,改善患者肺功能,并减少气道黏液的分泌,其机制可能是抑制IL-8、IL-10和NE,从而减少气道炎症。展开更多
目的:观察三子养亲汤合半夏厚朴汤加减治疗急性加重期慢性阻塞性肺疾病(acuteexacerbation of chronic obstructive pulmonary disease,AECOPD)痰浊阻肺证的临床疗效。方法:116例AECOPD痰浊阻肺证患者随机分为治疗组和对照组各58例。对...目的:观察三子养亲汤合半夏厚朴汤加减治疗急性加重期慢性阻塞性肺疾病(acuteexacerbation of chronic obstructive pulmonary disease,AECOPD)痰浊阻肺证的临床疗效。方法:116例AECOPD痰浊阻肺证患者随机分为治疗组和对照组各58例。对照组参照西医AECOPD临床指南制定的用药进行治疗,治疗组在对照组治疗基础上给予三子养亲汤合半夏厚朴汤。治疗两周后比较两组患者的临床疗效、中医证候积分、生活质量评分及肺功能指标。结果:治疗后,治疗组有效率为93.10%,对照组为77.58%,两组比较,差异有统计学意义(P<0.05)。治疗组中医证候积分、自我评估测试问卷(COPD assessment test,CAT)评分均显著低于对照组,差异有统计学意义(P<0.05)。两组患者用力肺活量(forcedvitalcapacity,FVC)比较,差异无统计学意义(P>0.05);治疗组第1秒用力呼气容积(forcedexpiratory volume in the first second,FEV1)、FEV1/FVC改善程度显著优于对照组,差异均有统计学意义(P<0.05)。结论:三子养亲汤合半夏厚朴汤加减治疗AECOPD痰浊阻肺证患者临床疗效显著,可显著提高患者生活质量,改善患者肺功能。展开更多
基金Science and Technology Planning Project of Lanzhou City(2018-1-117).
文摘[Objectives]To explore the molecular mechanism of Sanzi Yangqin Decoction in the treatment of bronchial asthma based on network pharmacology and molecular docking.[Methods]The components of Fructus Perillae,Semen Raphani and Semen Sinapis three traditional Chinese medicine-related components and targets of Feiduqing Sanzi Yangqin Decoction were obtained using Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),and the targets of bronchial asthma were obtained using Genecards and OMIM databases.Sanzi Yangqin Decoction"drug-active ingredient-target-disease"network was established with the aid of Cytoscape 3.7.2 software and network topology analysis was carried out.The gene ontology(GO)function enrichment analysis and the KEGG pathway enrichment analysis were performed by DAVID.The top 3 components and targets in the network topology analysis were respectively molecularly docked.[Results]Through network analysis,4 key active components were obtained,mainly luteolin,arachidonic acid,β-carotene,etc.;5 key targets,mainly NCOA2,PGR,PTGS2,etc.Through GO analysis,523 items(P<0.05)were obtained,including 396 items in biological process(BP),53 items in cell composition(CC),and 74 items in molecular function(MF).KEGG analysis generated 144 signal pathways(P<0.05),involving PI3K-Akt signal pathway,human cytomegalovirus infection,Kaposi's sarcoma-associated herpes virus infection,proteoglycans in cancer,prostate cancer,etc.The results of molecular docking showed that core active compounds such as luteolin andβ-carotene in Sanzi Yangqin Decoction had good affinity with NCOA2,PGR,PTGS2 and other target genes,which were similar to clinically recommended chemical drugs.[Conclusions]The active compounds luteolin and carotene in Sanzi Yangqin Decoction may mainly bind to targets such as NCOA2,PGR,PTGS2,and regulate multiple signaling pathways such as PI3K-Akt to play a role in treating bronchial asthma.It is intended to provide new ideas for the clinical application and research of Sanzi Yangqi
文摘目的:观察三子养亲汤加味对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)稳定期患者的临床疗效、活动耐力及气道黏液高分泌的影响。方法:90例COPD稳定期患者随机分为对照组和观察组各45例。对照组患者给予常规西医治疗,包括氨茶碱缓释片和沙丁胺醇气雾剂等;观察组在对照组治疗基础上联用三子养亲汤加味治疗,2周为1个疗程,连续治疗3个疗程。治疗后,比较两组患者活动耐力、中医证候评分、肺功能[用力肺活量(forced vital capacity,FVC)、第1秒用力呼气容积(the first second force expiratory volume,FEV1)和FEV1%]、血清炎性因子[白细胞介素-8(interleukin-8,IL-8)、IL-10和中性粒细胞弹性蛋白酶(neutrophil elastase,NE)]的含量,并评价疗效。结果:治疗后,两组患者FEV1和FEV1%均升高,其中观察组FEV1高于对照组,差异有统计学意义(P<0.05);治疗后,两组患者6分钟步行距离(6-minutes walk desease,6-MWD)均增加,观察组6-MWD高于对照组(P<0.05);治疗后,两组患者IL-8和IL-10均下降,且观察组低于对照组(P<0.05);观察组NE明显下降,且低于对照组(P<0.05);治疗后,两组患者中医证候评分均降低,且观察组评分低于对照组(P<0.05);观察组有效率为93.33%,对照组有效率为77.78%,两组有效率比较,差异有统计学意义(P<0.05)。结论:三子养亲汤加味治疗稳定期COPD疗效显著,可以明显提高患者活动耐力,改善患者肺功能,并减少气道黏液的分泌,其机制可能是抑制IL-8、IL-10和NE,从而减少气道炎症。
文摘目的:观察三子养亲汤合半夏厚朴汤加减治疗急性加重期慢性阻塞性肺疾病(acuteexacerbation of chronic obstructive pulmonary disease,AECOPD)痰浊阻肺证的临床疗效。方法:116例AECOPD痰浊阻肺证患者随机分为治疗组和对照组各58例。对照组参照西医AECOPD临床指南制定的用药进行治疗,治疗组在对照组治疗基础上给予三子养亲汤合半夏厚朴汤。治疗两周后比较两组患者的临床疗效、中医证候积分、生活质量评分及肺功能指标。结果:治疗后,治疗组有效率为93.10%,对照组为77.58%,两组比较,差异有统计学意义(P<0.05)。治疗组中医证候积分、自我评估测试问卷(COPD assessment test,CAT)评分均显著低于对照组,差异有统计学意义(P<0.05)。两组患者用力肺活量(forcedvitalcapacity,FVC)比较,差异无统计学意义(P>0.05);治疗组第1秒用力呼气容积(forcedexpiratory volume in the first second,FEV1)、FEV1/FVC改善程度显著优于对照组,差异均有统计学意义(P<0.05)。结论:三子养亲汤合半夏厚朴汤加减治疗AECOPD痰浊阻肺证患者临床疗效显著,可显著提高患者生活质量,改善患者肺功能。
