Electron spin resonance (ESR) technique has been widely used as a modern biophysicalmethod in every field of life science. Spin probe method is a special ESR technique. Inorder to extend the biological application of ...Electron spin resonance (ESR) technique has been widely used as a modern biophysicalmethod in every field of life science. Spin probe method is a special ESR technique. Inorder to extend the biological application of the spin probe method.a nitroxide radicalanalogous to imidazole F was synthesized:展开更多
The polymer-polymer interaction parameters of STVPh/PPC blends were calculated by Hildebrand solubility parameter. Accordingly the miscibility of the blends was predicted. The segment motion and miscibility of blends ...The polymer-polymer interaction parameters of STVPh/PPC blends were calculated by Hildebrand solubility parameter. Accordingly the miscibility of the blends was predicted. The segment motion and miscibility of blends were also investigated by electron spin resonance(ESR) spin probe method. ESR spectra were measured as a function of temperature and the hydroxyl content in STVPh. For all blends, two spectral components with different rates of motion, a "fast" and a "slow" component were observed. This result showed that the nitrooxide radical existed in two different matrixes, a more flexible PPC-rich and a more rigid STVPh-rich micro domain. In addition, T 5mT and rotational correlation time τ c and activation energy E a were obtained from ESR spectra, which indicated the hydrogen bonding between the carbonyl and ether oxygen in PPC and the hydroxyl in STVPh was enhanced with increasing the hydroxyl content in STVPh.展开更多
NITRIC oxide (NO) is the important cell messenger molecule that regulates the central ner-vous, cardiovascular, endocrine and immunity systems. The study of NO not only canexplain many physiological phenomena, but als...NITRIC oxide (NO) is the important cell messenger molecule that regulates the central ner-vous, cardiovascular, endocrine and immunity systems. The study of NO not only canexplain many physiological phenomena, but also can provide new ways to prevent and treat alot of diseases. However, at present, most of the methods for assaying NO produced by or-ganism have some defects, for example, low sensitivity, no specificity, no convenience.展开更多
Oxygen spin probe method is a modern biophysics technique, which is applied to theobservation of metabolic process of oxygen in cells. Here, another application of thismethod is developed in our current work, in which...Oxygen spin probe method is a modern biophysics technique, which is applied to theobservation of metabolic process of oxygen in cells. Here, another application of thismethod is developed in our current work, in which the trace sample reaction’s kineticswas studied.Nitroxide free radical 3-carbamoyl-2, 2, 5, 5。展开更多
基金Project supported by the National Natural Science Foundation of China.
文摘Electron spin resonance (ESR) technique has been widely used as a modern biophysicalmethod in every field of life science. Spin probe method is a special ESR technique. Inorder to extend the biological application of the spin probe method.a nitroxide radicalanalogous to imidazole F was synthesized:
文摘The polymer-polymer interaction parameters of STVPh/PPC blends were calculated by Hildebrand solubility parameter. Accordingly the miscibility of the blends was predicted. The segment motion and miscibility of blends were also investigated by electron spin resonance(ESR) spin probe method. ESR spectra were measured as a function of temperature and the hydroxyl content in STVPh. For all blends, two spectral components with different rates of motion, a "fast" and a "slow" component were observed. This result showed that the nitrooxide radical existed in two different matrixes, a more flexible PPC-rich and a more rigid STVPh-rich micro domain. In addition, T 5mT and rotational correlation time τ c and activation energy E a were obtained from ESR spectra, which indicated the hydrogen bonding between the carbonyl and ether oxygen in PPC and the hydroxyl in STVPh was enhanced with increasing the hydroxyl content in STVPh.
文摘NITRIC oxide (NO) is the important cell messenger molecule that regulates the central ner-vous, cardiovascular, endocrine and immunity systems. The study of NO not only canexplain many physiological phenomena, but also can provide new ways to prevent and treat alot of diseases. However, at present, most of the methods for assaying NO produced by or-ganism have some defects, for example, low sensitivity, no specificity, no convenience.
基金Project supported by the National Natural Science Foundation of China.
文摘Oxygen spin probe method is a modern biophysics technique, which is applied to theobservation of metabolic process of oxygen in cells. Here, another application of thismethod is developed in our current work, in which the trace sample reaction’s kineticswas studied.Nitroxide free radical 3-carbamoyl-2, 2, 5, 5。
