目的探讨花边莲提取液对人肺癌SPC-A-1细胞凋亡相关基因survivin和caspase-3表达的影响。方法采用体外培养人肺癌SPC-A-1细胞,实验随机分为正常对照组、花边莲处理组(浓度分别为12.5,25和50 m l/L)和顺铂(DDp25mg/L)组,半定量RT-PCR检测...目的探讨花边莲提取液对人肺癌SPC-A-1细胞凋亡相关基因survivin和caspase-3表达的影响。方法采用体外培养人肺癌SPC-A-1细胞,实验随机分为正常对照组、花边莲处理组(浓度分别为12.5,25和50 m l/L)和顺铂(DDp25mg/L)组,半定量RT-PCR检测survivin和caspase-3 mRNA表达水平,二步法免疫组化检测Survivin和Caspase-3蛋白表达变化。结果与正常对照组相比,花边莲各浓度组caspase-3 mRNA和蛋白表达显著上升(P<0.01),survivin mRNA和蛋白表达明显下降(P<0.01)。结论花边莲提取液诱导SPC-A-1细胞凋亡的分子机理可能与上调caspase-3基因表达和下调survivin基因表达有关。展开更多
AIM: In an effort to identify novel, small molecules which can affect the proliferation of lung cancer cells, F-01A, a polyether antibiotic isolated from the fermentation broth of Streptomyces was tested. METHOD: F-01...AIM: In an effort to identify novel, small molecules which can affect the proliferation of lung cancer cells, F-01A, a polyether antibiotic isolated from the fermentation broth of Streptomyces was tested. METHOD: F-01A was tested for its antitumor properties on the lung cancer cell line SPC-A-1, at six doses(0.1, 0.5, 1, 2.5, and 5 μmol·L-1), using various cellular assays. Cell viability was measured by the MTT assay, Hochest 33258 was used to study nuclear morphology; DNA ladder and the loss of mitochondrial membrane potential were also evaluated. RESULTS: F-01A induces apoptosis against SPC-A-1 cells in a dose-dependent manner. The IC50 is 0.65 μmol·L-1, and the inhibition at 5 μmol·L-1 is 87.89%. Further, JC-1 staining indicates F-01A could induce the loss of mitochondrial membrane potential, and the DNA fragment is evident. CONCLUSION: Mechanistic analysis showed that F-01A induced apoptosis of cancer cells probably in the mitochondrial pathway. The antitumor actions of F-01A involve activation of the apoptotic pathway against SPC-A-1 cells, and it may be valuable for further drug development.展开更多
基金supported by the National Nature Science Foundation of China(Nos.31360069,21162009)the Program for New Century Excellent Talents in University(No.NCET-08-0656)
文摘AIM: In an effort to identify novel, small molecules which can affect the proliferation of lung cancer cells, F-01A, a polyether antibiotic isolated from the fermentation broth of Streptomyces was tested. METHOD: F-01A was tested for its antitumor properties on the lung cancer cell line SPC-A-1, at six doses(0.1, 0.5, 1, 2.5, and 5 μmol·L-1), using various cellular assays. Cell viability was measured by the MTT assay, Hochest 33258 was used to study nuclear morphology; DNA ladder and the loss of mitochondrial membrane potential were also evaluated. RESULTS: F-01A induces apoptosis against SPC-A-1 cells in a dose-dependent manner. The IC50 is 0.65 μmol·L-1, and the inhibition at 5 μmol·L-1 is 87.89%. Further, JC-1 staining indicates F-01A could induce the loss of mitochondrial membrane potential, and the DNA fragment is evident. CONCLUSION: Mechanistic analysis showed that F-01A induced apoptosis of cancer cells probably in the mitochondrial pathway. The antitumor actions of F-01A involve activation of the apoptotic pathway against SPC-A-1 cells, and it may be valuable for further drug development.
