Background Overexpression of breast cancer-specific gene 1 （SNCG） is associated with poor prognosis in advanced breast cancer patients. This study aimed to determine the effects of SNCG knockdown in breast cancer ce...Background Overexpression of breast cancer-specific gene 1 （SNCG） is associated with poor prognosis in advanced breast cancer patients. This study aimed to determine the effects of SNCG knockdown in breast cancer cells by using small hairpin RNA （shRNA）.Methods Four different SNCG shRNA oligonucleotides were designed and chemically synthesized to construct mammalian expression vectors. These vectors were then stably transfected into a breast cancer MCF-7 cell line to knockdown SNCG expression. After SNCG knockdown was confirmed, the stable cell lines were inoculated into nude mice. SNCG mRNA and protein expressions were analyzed by semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry, respectively in both the stable cell lines and xenografts.Results All four SNCG shRNA constructs significantly reduced SNCG mRNA and protein levels in MCF-7 cells, as compared to the unrelated sequence control shRNA and the liposome control mice （P〈0.05）. SNCG-knockdown MCF-7cells formed significantly smaller tumor masses than cells expressing the unrelated sequence control or the liposome control mice （P〈0.05）.Conclusion SNCG shRNA effectively suppressed breast cancer cell formation in vivo and may be a useful clinical strategy to control breast cancer展开更多
Objective:To investigate the correlation between periostin and SNCG and esophageal cancer invasion,infiltration and apoptosis.Methods:A total of 78 cases esophageal surgical resection specimens were collected,expressi...Objective:To investigate the correlation between periostin and SNCG and esophageal cancer invasion,infiltration and apoptosis.Methods:A total of 78 cases esophageal surgical resection specimens were collected,expression of periostin and SNCG in esophageal cancer were detected. Effect of periostin and SNCG in esophageal carcinoma invasion and infiltration was analyzed. Results:The upregulated rate of periostin had significant difference in esophageal cancer tissues (39.74%),adjacent tissues(17.86%) and normal tissues(0.00%);The positive expression rates of SNCG had significant difference in esophageal cancer tissues(61.54%),adjacent tissues(32.14% and normal tissues 11.96%);The upreguialed rate of perioslin had a significant correlation with lymph node metastasis,adventitia invasion.TNM stage:The positive expression rates of SNCG had a significant correlation with differentiation degree,lymph node metastasis,adventitia invasion.TNM stage:Apoptosis index ol the positive of expression ol SNCG of esophageal cancer tissue(4.541±2.267) was significantly lower than that of the negative expression(7.316±2.582) (P【0.05).Conclusions:SNCG may play an important role in invasion,infiltration and apoptosis of esophageal cancer and serve as larget spots in the targeted therapy of esophageal cancer.展开更多
文摘Background Overexpression of breast cancer-specific gene 1 （SNCG） is associated with poor prognosis in advanced breast cancer patients. This study aimed to determine the effects of SNCG knockdown in breast cancer cells by using small hairpin RNA （shRNA）.Methods Four different SNCG shRNA oligonucleotides were designed and chemically synthesized to construct mammalian expression vectors. These vectors were then stably transfected into a breast cancer MCF-7 cell line to knockdown SNCG expression. After SNCG knockdown was confirmed, the stable cell lines were inoculated into nude mice. SNCG mRNA and protein expressions were analyzed by semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry, respectively in both the stable cell lines and xenografts.Results All four SNCG shRNA constructs significantly reduced SNCG mRNA and protein levels in MCF-7 cells, as compared to the unrelated sequence control shRNA and the liposome control mice （P〈0.05）. SNCG-knockdown MCF-7cells formed significantly smaller tumor masses than cells expressing the unrelated sequence control or the liposome control mice （P〈0.05）.Conclusion SNCG shRNA effectively suppressed breast cancer cell formation in vivo and may be a useful clinical strategy to control breast cancer
基金supported by China International Medical Forndation special Foundation(Grant No:CIMF-F-H001-225)Science and technology commision of Shanghai medical guide Foundations(Grant No:114119a7700)
文摘Objective:To investigate the correlation between periostin and SNCG and esophageal cancer invasion,infiltration and apoptosis.Methods:A total of 78 cases esophageal surgical resection specimens were collected,expression of periostin and SNCG in esophageal cancer were detected. Effect of periostin and SNCG in esophageal carcinoma invasion and infiltration was analyzed. Results:The upregulated rate of periostin had significant difference in esophageal cancer tissues (39.74%),adjacent tissues(17.86%) and normal tissues(0.00%);The positive expression rates of SNCG had significant difference in esophageal cancer tissues(61.54%),adjacent tissues(32.14% and normal tissues 11.96%);The upreguialed rate of perioslin had a significant correlation with lymph node metastasis,adventitia invasion.TNM stage:The positive expression rates of SNCG had a significant correlation with differentiation degree,lymph node metastasis,adventitia invasion.TNM stage:Apoptosis index ol the positive of expression ol SNCG of esophageal cancer tissue(4.541±2.267) was significantly lower than that of the negative expression(7.316±2.582) (P【0.05).Conclusions:SNCG may play an important role in invasion,infiltration and apoptosis of esophageal cancer and serve as larget spots in the targeted therapy of esophageal cancer.
