Nucleolus is the most prominent subnuclear structure, which performs a wide variety of functions in the eukaryotic cellular processes. In order to understand the structural and functional role of the nucleoli in bovin...Nucleolus is the most prominent subnuclear structure, which performs a wide variety of functions in the eukaryotic cellular processes. In order to understand the structural and functional role of the nucleoli in bovine cells, we analyzed the proteomic composition of the bovine nucleoli. The nucleoli were isolated from Madin Darby bovine kidney cells and subjected to proteomic analysis by LC-MS/MS after fractionation by SDS-PAGE and strong cation exchange chromatography. Analysis of the data using the Mascot database search and the GPM database search identified 311 proteins in the bovine nucleoli, which contained 22 proteins previously not identified in the proteomic analysis of human nucleoli. Analysis of the identified proteins using the GoMiner software suggested that the bovine nucleoli contained proteins involved in ribosomal biogenesis, cell cycle control, transcriptional, translational and post-translational regulation, transport, and structural organization.展开更多
The paper analyzes the benefit and risk of liabilities operation in telecom enterprises and the advantages of internal and external conditions in liabilities operation. The quantitative model of liabilities scale is s...The paper analyzes the benefit and risk of liabilities operation in telecom enterprises and the advantages of internal and external conditions in liabilities operation. The quantitative model of liabilities scale is studied by analyzing the factors that act on the liabilities scale.展开更多
建立了高效液相色谱法测定奶粉中双氰胺含量的方法。样品经三氯乙酸沉淀蛋白质和脂肪,在流动相为1.7%磷酸二氢铵的条件下用磺酸基阳离子交换色谱柱（SCX）分离,紫外检测波长218 nm,流速0.8 m L/min,标准曲线法定量。结果表明,双氰胺的...建立了高效液相色谱法测定奶粉中双氰胺含量的方法。样品经三氯乙酸沉淀蛋白质和脂肪,在流动相为1.7%磷酸二氢铵的条件下用磺酸基阳离子交换色谱柱（SCX）分离,紫外检测波长218 nm,流速0.8 m L/min,标准曲线法定量。结果表明,双氰胺的回收率和样品重复测定相对标准偏差分别是97.54%-99.24%和0.01%,方法检测限0.05 mg/kg。该方法无需进行固相萃取,快速灵敏,简便准确,适用于奶粉中双氰胺含量的检测。展开更多
[Objective] The aim was to conduct HPLC analysis on Cordyceps Sinensis/Betaine compound feed nutriment. [Method] Cordyceps Sinensis/Betaine compound feed nutriment was under HPLC analysis to determine separation of Co...[Objective] The aim was to conduct HPLC analysis on Cordyceps Sinensis/Betaine compound feed nutriment. [Method] Cordyceps Sinensis/Betaine compound feed nutriment was under HPLC analysis to determine separation of Cordyceps Sinensis effluent and betaine. [Result] Different compositions in Cordyceps Sinensis/etaine compound feed nutriment would be well separated by the method. [Conclusion] The method provides a suitable platform of separation and analysis for Cordyceps Sinensis /Betaine compound feed nutriment.展开更多
文摘Nucleolus is the most prominent subnuclear structure, which performs a wide variety of functions in the eukaryotic cellular processes. In order to understand the structural and functional role of the nucleoli in bovine cells, we analyzed the proteomic composition of the bovine nucleoli. The nucleoli were isolated from Madin Darby bovine kidney cells and subjected to proteomic analysis by LC-MS/MS after fractionation by SDS-PAGE and strong cation exchange chromatography. Analysis of the data using the Mascot database search and the GPM database search identified 311 proteins in the bovine nucleoli, which contained 22 proteins previously not identified in the proteomic analysis of human nucleoli. Analysis of the identified proteins using the GoMiner software suggested that the bovine nucleoli contained proteins involved in ribosomal biogenesis, cell cycle control, transcriptional, translational and post-translational regulation, transport, and structural organization.
文摘The paper analyzes the benefit and risk of liabilities operation in telecom enterprises and the advantages of internal and external conditions in liabilities operation. The quantitative model of liabilities scale is studied by analyzing the factors that act on the liabilities scale.
文摘建立了高效液相色谱法测定奶粉中双氰胺含量的方法。样品经三氯乙酸沉淀蛋白质和脂肪,在流动相为1.7%磷酸二氢铵的条件下用磺酸基阳离子交换色谱柱（SCX）分离,紫外检测波长218 nm,流速0.8 m L/min,标准曲线法定量。结果表明,双氰胺的回收率和样品重复测定相对标准偏差分别是97.54%-99.24%和0.01%,方法检测限0.05 mg/kg。该方法无需进行固相萃取,快速灵敏,简便准确,适用于奶粉中双氰胺含量的检测。
文摘[Objective] The aim was to conduct HPLC analysis on Cordyceps Sinensis/Betaine compound feed nutriment. [Method] Cordyceps Sinensis/Betaine compound feed nutriment was under HPLC analysis to determine separation of Cordyceps Sinensis effluent and betaine. [Result] Different compositions in Cordyceps Sinensis/etaine compound feed nutriment would be well separated by the method. [Conclusion] The method provides a suitable platform of separation and analysis for Cordyceps Sinensis /Betaine compound feed nutriment.
