[Objectives] To establish a method for the determination of gallic acid in Shuijia Zhixue capsule and Rotala rotundifolia .[Methods] High performance liquid chromatography (HPLC) and Phenomenex C 18 column were used, ...[Objectives] To establish a method for the determination of gallic acid in Shuijia Zhixue capsule and Rotala rotundifolia .[Methods] High performance liquid chromatography (HPLC) and Phenomenex C 18 column were used, the mobile phase was 0.1% phosphoric acid∶ methanol=85∶ 15, the flow rate was 0.5 mL/min, and the detection wavelength was 270 nm.[Results] In the range of (2-25)μg/mL , the concentration of gallic acid had a good linear relationship with the peak area, r =0.990 8;the average recovery was 96.88%.[Conclusions] The method was simple, sensitive and reproducible. It provided a basis for the analysis of gallic acid in Rotala rotundifolia and Shuijia Zhixue capsule and the establishment of quality detection methods in the future.展开更多
[Objectives] To evaluate the hemostatic and anti-inflammatory activities of water extract and ethanol extract of Rotala rotundifolia and Shuijia Zhixue capsule. [Methods]The hemostatic activity of the extracts was eva...[Objectives] To evaluate the hemostatic and anti-inflammatory activities of water extract and ethanol extract of Rotala rotundifolia and Shuijia Zhixue capsule. [Methods]The hemostatic activity of the extracts was evaluated by the changes of the bleeding time( BT),coagulation time( CT) and coagulation parameters including prothrombin time( PT),activated partial thromboplastin time( APTT) and thrombin time( TT) in mice or rats. The anti-inflammatory activity of the extracts was evaluated by carrageenan-induced foot edema,arachidonic acidinduced ear edema and xylene-induced ear edema models in rats. The content of gallic acid in ethanol extracts of R. rotundifolia and Shuijia Zhixue capsule was studied by HPLC to explore the correlation between gallic acid and anti-inflammatory activity. [Results]In hemostasis experiment,the ethanol extracts of R. rotundifolia and Shuijia Zhixue capsules showed significant shortening effects on BT,CT,PT and TT( P <0. 01-0. 05). The water extracts of R. rotundifolia and Shuijia Zhixue capsules had significant shortening effects on CT,PT and TT( P <0. 05),while the water extracts of R. rotundifolia had no hemostasis effect. In anti-inflammatory experiment in vivo,the two ethanol extracts showed significant shortening effects on CT,PT and TT and in the induction test of carrageenan,arachidonic acid and xylene,the inhibitory effects were significant( P < 0. 01-0. 05). The water extract from Shuijia Zhixue capsules had significant inhibitory effects on carrageenan-induced foot edema in rats( P < 0. 05),but the anti-inflammatory effects of water extract from R. rotundifolia were not significant. The content of gallic acid in R. rotundifolia and Shuijia Zhixue capsules were( 0. 318 62 ± 0. 009 8) and( 6. 295 2 ± 0. 162 6) mg/g,respectively.Among them,the content of gallic acid in Shuijia Zhixue capsule was significantly correlated with the anti-inflammatory activity of carrageenaninduced foot edema model. [Conclusions] Ethanol extract of R. rotundifolia and Shuijia Zhixue capsules has s展开更多
基金Supported by the Project of Sichuan Provincial Department of Science and Technology(2017SZYZF0012)Research Program Foundation of Science and Technology Bureau of Liangshan Yi Autonomous Prefecture(15yyjs0002)
文摘[Objectives] To establish a method for the determination of gallic acid in Shuijia Zhixue capsule and Rotala rotundifolia .[Methods] High performance liquid chromatography (HPLC) and Phenomenex C 18 column were used, the mobile phase was 0.1% phosphoric acid∶ methanol=85∶ 15, the flow rate was 0.5 mL/min, and the detection wavelength was 270 nm.[Results] In the range of (2-25)μg/mL , the concentration of gallic acid had a good linear relationship with the peak area, r =0.990 8;the average recovery was 96.88%.[Conclusions] The method was simple, sensitive and reproducible. It provided a basis for the analysis of gallic acid in Rotala rotundifolia and Shuijia Zhixue capsule and the establishment of quality detection methods in the future.
基金Supported by the Project of Sichuan Provincial Science and Technology Department(2017SZYZF0012)the Project of Science and Technology Bureau of Sichuan Liangshan Yi Autonomous Prefecture(15yyjs0002)
文摘[Objectives] To evaluate the hemostatic and anti-inflammatory activities of water extract and ethanol extract of Rotala rotundifolia and Shuijia Zhixue capsule. [Methods]The hemostatic activity of the extracts was evaluated by the changes of the bleeding time( BT),coagulation time( CT) and coagulation parameters including prothrombin time( PT),activated partial thromboplastin time( APTT) and thrombin time( TT) in mice or rats. The anti-inflammatory activity of the extracts was evaluated by carrageenan-induced foot edema,arachidonic acidinduced ear edema and xylene-induced ear edema models in rats. The content of gallic acid in ethanol extracts of R. rotundifolia and Shuijia Zhixue capsule was studied by HPLC to explore the correlation between gallic acid and anti-inflammatory activity. [Results]In hemostasis experiment,the ethanol extracts of R. rotundifolia and Shuijia Zhixue capsules showed significant shortening effects on BT,CT,PT and TT( P <0. 01-0. 05). The water extracts of R. rotundifolia and Shuijia Zhixue capsules had significant shortening effects on CT,PT and TT( P <0. 05),while the water extracts of R. rotundifolia had no hemostasis effect. In anti-inflammatory experiment in vivo,the two ethanol extracts showed significant shortening effects on CT,PT and TT and in the induction test of carrageenan,arachidonic acid and xylene,the inhibitory effects were significant( P < 0. 01-0. 05). The water extract from Shuijia Zhixue capsules had significant inhibitory effects on carrageenan-induced foot edema in rats( P < 0. 05),but the anti-inflammatory effects of water extract from R. rotundifolia were not significant. The content of gallic acid in R. rotundifolia and Shuijia Zhixue capsules were( 0. 318 62 ± 0. 009 8) and( 6. 295 2 ± 0. 162 6) mg/g,respectively.Among them,the content of gallic acid in Shuijia Zhixue capsule was significantly correlated with the anti-inflammatory activity of carrageenaninduced foot edema model. [Conclusions] Ethanol extract of R. rotundifolia and Shuijia Zhixue capsules has s
