研究金黄色葡萄球菌活的非可培养状态(viable but non-culturable state,VBNC)的诱导和复苏方法。采用不同pH、温度、盐度、不同浓度的食品防腐剂山梨酸钾处理进行诱导;复苏则采用了直接升温处理、添加液体培养基升温处理、添加酵母液...研究金黄色葡萄球菌活的非可培养状态(viable but non-culturable state,VBNC)的诱导和复苏方法。采用不同pH、温度、盐度、不同浓度的食品防腐剂山梨酸钾处理进行诱导;复苏则采用了直接升温处理、添加液体培养基升温处理、添加酵母液升温处理、添加吐温升温处理的方法。结果表明,经过不同温度和山梨酸钾诱导后的金黄色葡萄球菌进入了VBNC状态;其中诱导时间最短(76d)的条件是0.5mmol/L山梨酸钾、-20℃寡营养条件;采用BHI培养基升温的方法,以及添加0.5%和1%的吐温20和吐温80的培养液升温方法使进入VBNC状态的金黄色葡萄球菌得到复苏。VBNC状态的金黄色葡萄球菌在形态上发生了变化;复苏后的菌株除尿素酶阴性外,其他代谢特征均与标准菌株相同。展开更多
利用16S rDNA序列分析法测得实验室保藏的乳酸菌的16S rDNA序列,并与基因库中基因序列进行同源性比较,经鉴定此菌为植物乳杆菌。并研究了植物乳杆菌进入活的非可培养状态(Viable but Non-culturable,VBNC)的诱导条件和复苏条件。结果显...利用16S rDNA序列分析法测得实验室保藏的乳酸菌的16S rDNA序列,并与基因库中基因序列进行同源性比较,经鉴定此菌为植物乳杆菌。并研究了植物乳杆菌进入活的非可培养状态(Viable but Non-culturable,VBNC)的诱导条件和复苏条件。结果显示,在诱导条件为MRS液体培养基、pH5.5～6.2、温度-20℃和有氧环境时,植物乳杆菌在12d之后进入了VBNC状态;复苏条件为添加有6%吐温-80的MRS液体培养基和普通MRS液体培养基分别在48h和96h之内可以使VBNC的植物乳杆菌复苏。展开更多
AIM To determine morbidity and mortality in this specific patient group and also to assess for any independent associations between Do Not Resuscitate(DNR) status and increased post-operative morbidity and mortality.M...AIM To determine morbidity and mortality in this specific patient group and also to assess for any independent associations between Do Not Resuscitate(DNR) status and increased post-operative morbidity and mortality.METHODS We conducted a propensity score matched retrospective analysis using de-identified data from the American College of Surgeons' National Surgical Quality Improvement Project(ACS NSQIP) for all patients undergoing hip fracture surgery over a 7 year period in hospitals across the United States enrolled in ACS NSQIP with and without Do Not Resuscitate Status. We measured patient demographics including DNR status, co-morbidities, frailty and functional baseline, surgical and anaestheticprocedure data, post-operative morbidity/complications, length of stay, discharge destination and mortality.RESULTS Of 9218 patients meeting the inclusion criteria, 13.6% had a DNR status, 86.4% did not. Mortality was higher in the DNR status compared to the non-DNR group, at 15.3% vs 8.1% and propensity score matched multivariable analysis demonstrated that DNR status was independently associated with mortality(OR = 2.04, 95%CI: 1.46-2.86, P < 0.001). Additionally, analysis of the propensity score matched cohort demonstrated that DNR status was associated with a significant, but very small increased likelihood of post-operative complications(0.53 vs 0.43 complications per episode; OR = 1.21; 95%CI: 1.04-1.41, P = 0.004). Cardiopulmonary resuscitation and unplanned reintubation were significantly less likely in patients with DNR status.CONCLUSION Whilst DNR status patients had higher rates of postoperative complications and mortality, DNR status itself was not otherwise associated with increased morbidity. DNR status appears to increase 30-d mortality via ceilings of care in keeping with a DNR status, including withholding reintubation and cardiopulmonary resuscitation.展开更多
文摘研究金黄色葡萄球菌活的非可培养状态(viable but non-culturable state,VBNC)的诱导和复苏方法。采用不同pH、温度、盐度、不同浓度的食品防腐剂山梨酸钾处理进行诱导;复苏则采用了直接升温处理、添加液体培养基升温处理、添加酵母液升温处理、添加吐温升温处理的方法。结果表明,经过不同温度和山梨酸钾诱导后的金黄色葡萄球菌进入了VBNC状态;其中诱导时间最短(76d)的条件是0.5mmol/L山梨酸钾、-20℃寡营养条件;采用BHI培养基升温的方法,以及添加0.5%和1%的吐温20和吐温80的培养液升温方法使进入VBNC状态的金黄色葡萄球菌得到复苏。VBNC状态的金黄色葡萄球菌在形态上发生了变化;复苏后的菌株除尿素酶阴性外,其他代谢特征均与标准菌株相同。
文摘利用16S rDNA序列分析法测得实验室保藏的乳酸菌的16S rDNA序列,并与基因库中基因序列进行同源性比较,经鉴定此菌为植物乳杆菌。并研究了植物乳杆菌进入活的非可培养状态(Viable but Non-culturable,VBNC)的诱导条件和复苏条件。结果显示,在诱导条件为MRS液体培养基、pH5.5～6.2、温度-20℃和有氧环境时,植物乳杆菌在12d之后进入了VBNC状态;复苏条件为添加有6%吐温-80的MRS液体培养基和普通MRS液体培养基分别在48h和96h之内可以使VBNC的植物乳杆菌复苏。
文摘AIM To determine morbidity and mortality in this specific patient group and also to assess for any independent associations between Do Not Resuscitate(DNR) status and increased post-operative morbidity and mortality.METHODS We conducted a propensity score matched retrospective analysis using de-identified data from the American College of Surgeons' National Surgical Quality Improvement Project(ACS NSQIP) for all patients undergoing hip fracture surgery over a 7 year period in hospitals across the United States enrolled in ACS NSQIP with and without Do Not Resuscitate Status. We measured patient demographics including DNR status, co-morbidities, frailty and functional baseline, surgical and anaestheticprocedure data, post-operative morbidity/complications, length of stay, discharge destination and mortality.RESULTS Of 9218 patients meeting the inclusion criteria, 13.6% had a DNR status, 86.4% did not. Mortality was higher in the DNR status compared to the non-DNR group, at 15.3% vs 8.1% and propensity score matched multivariable analysis demonstrated that DNR status was independently associated with mortality(OR = 2.04, 95%CI: 1.46-2.86, P < 0.001). Additionally, analysis of the propensity score matched cohort demonstrated that DNR status was associated with a significant, but very small increased likelihood of post-operative complications(0.53 vs 0.43 complications per episode; OR = 1.21; 95%CI: 1.04-1.41, P = 0.004). Cardiopulmonary resuscitation and unplanned reintubation were significantly less likely in patients with DNR status.CONCLUSION Whilst DNR status patients had higher rates of postoperative complications and mortality, DNR status itself was not otherwise associated with increased morbidity. DNR status appears to increase 30-d mortality via ceilings of care in keeping with a DNR status, including withholding reintubation and cardiopulmonary resuscitation.
