Photoperiod-sensitive genie male sterile (PSGMS) rice is a very useful germplasm for hybrid rice development. It was first found as a spontaneous mutant in a japonic a cultivar 'Nongken 58' . pms3 on chromosom...Photoperiod-sensitive genie male sterile (PSGMS) rice is a very useful germplasm for hybrid rice development. It was first found as a spontaneous mutant in a japonic a cultivar 'Nongken 58' . pms3 on chromosome 12 was determined to be the locus where the original PSGMS mutation occurred, changing the normal cultivar Nongken 58 to PSGMS Nongken 58S. Large amounts of RAPD and AFLP analyses were also conducted for the fine mapping of the pms3 genomic region, which resulted in 4 molecular markers linked to pms3. Although these markers somewhat increased the marker density of this region, the pms3 locus is still located in a marker-sparse region.展开更多
利用16S rDNA克隆文库及限制性内切酶片段长度多态性（Restriction Fragment Length Polymorphism,RFLP）技术分析脆肉鲩鱼在冷藏条件下的特定腐败菌。分别提取新鲜鱼肉及腐败的脆肉鲩鱼肉样品中的细菌总DNA并利用通用引物27F/1492R进行...利用16S rDNA克隆文库及限制性内切酶片段长度多态性（Restriction Fragment Length Polymorphism,RFLP）技术分析脆肉鲩鱼在冷藏条件下的特定腐败菌。分别提取新鲜鱼肉及腐败的脆肉鲩鱼肉样品中的细菌总DNA并利用通用引物27F/1492R进行PCR扩增,所得PCR产物用于构建克隆文库,阳性克隆子用RFLP进行分析。结果表明：新鲜鱼肉的克隆文库中细菌种类比较丰富,达15种类型,而腐败鱼肉的细菌种类只有7种,且有2种分型的细菌占优势,共占克隆子总数的88.4%。序列比对分析表明,这2种优势菌的16S rDNA序列与假单孢菌属（Pseudomonas）细菌的核苷酸序列的相似性高达99%,说明脆肉鲩鱼在冷藏条件下的特定腐败菌是假单孢菌（Pseudomonassp.）。展开更多
基金Project supported by the National Program of High Technology Development of China and the Rockefeller Foundation.
文摘Photoperiod-sensitive genie male sterile (PSGMS) rice is a very useful germplasm for hybrid rice development. It was first found as a spontaneous mutant in a japonic a cultivar 'Nongken 58' . pms3 on chromosome 12 was determined to be the locus where the original PSGMS mutation occurred, changing the normal cultivar Nongken 58 to PSGMS Nongken 58S. Large amounts of RAPD and AFLP analyses were also conducted for the fine mapping of the pms3 genomic region, which resulted in 4 molecular markers linked to pms3. Although these markers somewhat increased the marker density of this region, the pms3 locus is still located in a marker-sparse region.
文摘应用限制性片段长度多态性（RFLP）、双向逆转录-聚合酶链式反应（BD-PCR）、序列分析等技术对我国部分地区柑橘衰退病毒（Citrus tristeza virus，CTV）柚类分离株进行了鉴定分析。结果表明：柚类以受相对单一的CTV株系侵染为主；柚类上的优势流行株系属于p25／Hin f ⅠRFLP6组群（占79．4％）和p23／BD-PCRⅢ组群（占84．1％）；在对柚类CTV分离株S051～S058及国外CTV分离株PB61、T30、T36、T385、SY568、VT、NUAGA的p23、p25基因序列分析中，S051与弱毒株PB61的同源性最高；S052与弱毒株T30、T385的同源性最高；S053、S054、S055、S056、S057、S058与其它CTV分离株的同源关系均较远，并在系统发生树上形成了独立的分枝。
