Background：Screening on multidrug-resistant tuberculosis （MDR-TB） has been limited to the serious TB subpopulations excluding the new TB patients.This study aimed to examine MDR-TB burden among the new TB patients....Background：Screening on multidrug-resistant tuberculosis （MDR-TB） has been limited to the serious TB subpopulations excluding the new TB patients.This study aimed to examine MDR-TB burden among the new TB patients.Methods：We conducted a study in Zhejiang Province during 2009-2013 to screen for MDR-TB patients among the low MDR-TB risk patients and five subpopulations of high MDR-TB risk patients.The number,prevalence,and trend of MDR-TB were compared while the logistic regression model was used to examine risk factors related to MDR-TB.Results：A total of 200 and 791 MDR-TB cases were,respectively,identified from the 9830 new TB cases and 2372 high-risk suspects who took MDR-TB screening from 2009 to 2013.The MDR-TB rates went down in both of the new TB patients and five MDR-TB high-risk groups over the study time,but the percentage of MDR-TB patients identified from the new TB patients in all diagnosed MDR-TB cases kept stable from 28.3% in 2011 to 27.0% in 2012 to 26.0% in 2013.Conclusions：The study indicated that MDR-TB burden among new TB patients was high,thus screening for MDR-TB among the new TB patients should be recommended in China as well as in the similar situation worldwide.展开更多
AIM： To elucidate the mechanism of multidrug resistance in retinoblastoma, and to acquire more insights into in vivo drug resistance.METHODS： Three anticancer drug resistant Y79 human RB cells were generated against...AIM： To elucidate the mechanism of multidrug resistance in retinoblastoma, and to acquire more insights into in vivo drug resistance.METHODS： Three anticancer drug resistant Y79 human RB cells were generated against vincristine, etoposide or carboplatin, which are used for conventional chemotherapy in RB. Primary cultures from enucleated eyes after chemotherapy（PCNC） were also prepared. Their chemosensitivity to chemotherapeutic agents（vincristine, etoposide and carboplatin） were measured using MTT assay. Western blot analysis was performed to evaluate the expression of p53, Bcl-2 and various multidrug resistant proteins in retinoblastoma cells.RESULTS： Following exposure to chemotherapeutic drugs, PCNC showed less sensitivity to drugs. No significant changes observed in the p53 expression, whereas Bcl-2 expression was found to be increased in the drug resistant cells as well as in PCNC. Increased expression of P-glycoprotein（P-gp） was observed in drug resistant Y79 cells; however there was no significant change in the expression of P-gp found between primary cultures of primarily enucleated eyes and PCNC. Multidrug resistance protein 1（Mrp-1） expression was found to be elevated in the drug resistant Y79 cells as well as in PCNC. No significant change in the expression of lung resistance associatedprotein（Lrp） was observed in the drug resistant Y79 cells as well as in PCNC.CONCLUSION： Our results suggest that multidrug resistant proteins are intrinsically present in retinoblastoma which causes treatment failure in managing retinoblastoma with chemotherapy.展开更多
目的:研究肺耐药相关蛋白(lung resistance related protein,LRP)、多药耐药相关蛋白(multidrug resistance,MRP)在肺癌中的表达及其临床意义。方法:免疫组织化学法检测62例肺癌组织LRP和MRP的表达情况。结果:62例肺癌组织中LRP、MRP的...目的:研究肺耐药相关蛋白(lung resistance related protein,LRP)、多药耐药相关蛋白(multidrug resistance,MRP)在肺癌中的表达及其临床意义。方法:免疫组织化学法检测62例肺癌组织LRP和MRP的表达情况。结果:62例肺癌组织中LRP、MRP的表达率分别为61.3%和70.0%,LRP和MRP共同阳性表达率为51.6%,LRP、MRP的阳性表达率在肺癌患者的年龄、性别、病理类型、分化程度及TNM分期差异均无统计学意义(P>0.05),且两者之间无相关关系(P>0.05)。结论:LRP、MRP与肺癌的固有性耐药有关,与肺癌的临床病理特征无明显关系,且两者在肺癌组织中的表达无相关关系。展开更多
文摘Background：Screening on multidrug-resistant tuberculosis （MDR-TB） has been limited to the serious TB subpopulations excluding the new TB patients.This study aimed to examine MDR-TB burden among the new TB patients.Methods：We conducted a study in Zhejiang Province during 2009-2013 to screen for MDR-TB patients among the low MDR-TB risk patients and five subpopulations of high MDR-TB risk patients.The number,prevalence,and trend of MDR-TB were compared while the logistic regression model was used to examine risk factors related to MDR-TB.Results：A total of 200 and 791 MDR-TB cases were,respectively,identified from the 9830 new TB cases and 2372 high-risk suspects who took MDR-TB screening from 2009 to 2013.The MDR-TB rates went down in both of the new TB patients and five MDR-TB high-risk groups over the study time,but the percentage of MDR-TB patients identified from the new TB patients in all diagnosed MDR-TB cases kept stable from 28.3% in 2011 to 27.0% in 2012 to 26.0% in 2013.Conclusions：The study indicated that MDR-TB burden among new TB patients was high,thus screening for MDR-TB among the new TB patients should be recommended in China as well as in the similar situation worldwide.
基金the support of Department of Biotechnology, Govt. of India
文摘AIM： To elucidate the mechanism of multidrug resistance in retinoblastoma, and to acquire more insights into in vivo drug resistance.METHODS： Three anticancer drug resistant Y79 human RB cells were generated against vincristine, etoposide or carboplatin, which are used for conventional chemotherapy in RB. Primary cultures from enucleated eyes after chemotherapy（PCNC） were also prepared. Their chemosensitivity to chemotherapeutic agents（vincristine, etoposide and carboplatin） were measured using MTT assay. Western blot analysis was performed to evaluate the expression of p53, Bcl-2 and various multidrug resistant proteins in retinoblastoma cells.RESULTS： Following exposure to chemotherapeutic drugs, PCNC showed less sensitivity to drugs. No significant changes observed in the p53 expression, whereas Bcl-2 expression was found to be increased in the drug resistant cells as well as in PCNC. Increased expression of P-glycoprotein（P-gp） was observed in drug resistant Y79 cells; however there was no significant change in the expression of P-gp found between primary cultures of primarily enucleated eyes and PCNC. Multidrug resistance protein 1（Mrp-1） expression was found to be elevated in the drug resistant Y79 cells as well as in PCNC. No significant change in the expression of lung resistance associatedprotein（Lrp） was observed in the drug resistant Y79 cells as well as in PCNC.CONCLUSION： Our results suggest that multidrug resistant proteins are intrinsically present in retinoblastoma which causes treatment failure in managing retinoblastoma with chemotherapy.
文摘目的:研究肺耐药相关蛋白(lung resistance related protein,LRP)、多药耐药相关蛋白(multidrug resistance,MRP)在肺癌中的表达及其临床意义。方法:免疫组织化学法检测62例肺癌组织LRP和MRP的表达情况。结果:62例肺癌组织中LRP、MRP的表达率分别为61.3%和70.0%,LRP和MRP共同阳性表达率为51.6%,LRP、MRP的阳性表达率在肺癌患者的年龄、性别、病理类型、分化程度及TNM分期差异均无统计学意义(P>0.05),且两者之间无相关关系(P>0.05)。结论:LRP、MRP与肺癌的固有性耐药有关,与肺癌的临床病理特征无明显关系,且两者在肺癌组织中的表达无相关关系。
