Detecting and segmenting salient objects from natural scenes, often referred to as salient object detection, has attracted great interest in computer vision. While many models have been proposed and several applicatio...Detecting and segmenting salient objects from natural scenes, often referred to as salient object detection, has attracted great interest in computer vision. While many models have been proposed and several applications have emerged, a deep understanding of achievements and issues remains lacking. We aim to provide a comprehensive review of recent progress in salient object detection and situate this field among other closely related areas such as generic scene segmentation, object proposal generation, and saliency for fixation prediction. Covering 228 publications, we survey i) roots, key concepts, and tasks, ii) core techniques and main modeling trends, and iii) datasets and evaluation metrics for salient object detection. We also discuss open problems such as evaluation metrics and dataset bias in model performance, and suggest future research directions.展开更多
There is no temperature effect in the southern Tibetan Plateau and South Asia to the south of the Tanggula Mountains. Amount effect has been observed at a few sampling stations accounting for about a half of the stati...There is no temperature effect in the southern Tibetan Plateau and South Asia to the south of the Tanggula Mountains. Amount effect has been observed at a few sampling stations accounting for about a half of the statistical stations. There is notable temperature effect in the middle and northern Tibetan Plateau and its adjacent Central Asia to the north of the Tanggula Mountains. Because vapor directly originates from low-latitude oceans, the relative heavy ( 18O with small variation characterizes the rainfall in South Asia. A sharp depletion of the stable isotopic compositions in precipitation takes place from Kyangjin on the southern slope of the Himalayas to the Tanggula Mountains in the middle plateau. From the Tanggula Mountains to the northern Tibetan Plateau, the ( 18O in precipitation increases with increasing latitude.展开更多
The Qinghai-Xizang Plateau is a unique physico-geographical region on the earth. As a whole, the spatial differentiation of physico-geographical regions of the plateau is mainly determined by topographic configuration...The Qinghai-Xizang Plateau is a unique physico-geographical region on the earth. As a whole, the spatial differentiation of physico-geographical regions of the plateau is mainly determined by topographic configuration and atmospheric circulation, warm and humid in the southeast, cold and arid in the northwest. The natural landscapes apppear in the following succession: forest → meadow → steppe → desert. The system of physico-geographical regions of the plateau is demarcated on the principle of bio-climate or the principle of three dimension zonality. Based on the thermal conditions, moisture regimes and variation in landform the Qinghai-Xizang Plateau is sequentially demarcated. The duration of mean daily temperature above 10℃ is the principal index, the subsidiary criterion is mean temperature of the warmest month, two temperature belts may be divided: plateau subpolar and plateau temperate. Annual aridity is taken as the principal index, subordinated by annual precipitation. Four moisture regional types are identified: humid, subhumid, semiarid and arid. A tentative scheme of 2 temperature belts, 10 natural zones and 28 physical districts has been proposed not including southern slopes of the East Himalayas (montane subtropical, humid).展开更多
AIM: To test the hypothesis to block VEGF expression of SMMC-7721 hepatoma cells may inhibit tumor growth using the rat hepatoma model. METHODS: Amplify the 200 VEGF cDNA fragment and insert it into human U6 gene cass...AIM: To test the hypothesis to block VEGF expression of SMMC-7721 hepatoma cells may inhibit tumor growth using the rat hepatoma model. METHODS: Amplify the 200 VEGF cDNA fragment and insert it into human U6 gene cassette in the reverse orientation transcribing small antisense RNA which could specifically interact with VEGF165, and VEGF121 mRNA. Construct the retroviral vector containing this antisense VEGF U6 cassette and package the replication-deficient recombinant retrovirus. SMMC-7721 cells were transduced with these virus and positive clones were selected with G418. PCR and Southern blot analysis were performed to determine if U6 cassette integrated into the genomic DNA of positive clone. Transfected tumor cells were evaluated for RNA expression by ribonuclease protection assays. The VEGF protein in the supernatant of parental tumor cells and genetically modified tumor cells was determined with ELISA. In vitro and in vivo growth properties of antisense VEGF cell clone in nude mice were analyzed. RESULTS: Restriction enzyme digestion and PCR sequencing verified that the antisense VEGF RNA retroviral vector was successfully constructed.After G418 selection, resistant SMMC-7721 cell clone was picked up. PCR and Southern blot analysis suggested that U6 cassette was integrated into the cell genomic DNA. Stable SMMC-7721 cell clone transduced with U6 antisense RNA cassette could express 200 bp small antisense VEGF RNA and secrete reduced levels of VEGF in culture condition. Production of VEGF by antisense transgene-expressing cells was 65+/-10 ng/L per 10(6) cells, 42045 ng/L per 10(6) cells in sense group and 485+/-30 ng/L per 10(6) cells in the negative control group, (P【 0.05). The antisense-VEGF cell clone appeared phenotypically indistinguishable from SMMC-7721 cells and SMMC-7721 cells transfected sense VEGF. The growth rate of the antisense-VEGF cell clone was the same as the control cells. When S.C. was implanted into nude mice, growth of antisense-VEGF cell lines was greatly inhibited compared with co展开更多
AIM: To kill CEA positive colorectal carcinoma cells specifically using the E coli cytosine deaminase (CD) suicide gene, a new replication-deficient recombinant adenoviral vector was constructed in which CD gene was c...AIM: To kill CEA positive colorectal carcinoma cells specifically using the E coli cytosine deaminase (CD) suicide gene, a new replication-deficient recombinant adenoviral vector was constructed in which CD gene was controlled under CEA promoter and its in vitro cytotoxic effects were evaluated. METHODS: Shuttle plasmid containing CD gene and regulatory sequence of the CEA gene was constructed and recombined with the right arm of adenovirus genome DNA in 293 cell strain. Dot blotting and PCR were used to identify positive plaques. The purification of adenovirus was performed with ultra-concentration in CsCl step gradients and the titration was measured with plaque formation assay. Cytotoxic effects were assayed with MTT method, The fifty percent inhibition concentration (IC(50)) of 5-FC was calculated using a curve-fitting parameter. The human colorectal carcinoma cell line, which was CEA-producing, and the CEA-nonproducing Hela cell line were applied in cytological tests. An established recombinant adenovirus vector AdCMVCD, in which the CD gene was controlled under CMV promoter, was used as virus control. Quantitative results were expressed as the mean +/- SD of the mean. Statistical analysis was performed using ANOVA test. RESULTS: The desired recombinant adenovirus vector was named AdCEACD. The results of dot blotting and PCR showed that the recombinant adenovirus contained CEA promoter and CD gene. Virus titer was about 5.0 X 10(14)pfu/L(-1) after purification. The CEA-producing Lovo cells were sensitive to 5-FC and had the same cytotoxic effect after infection with AdCEACD and AdCMVCD (The IC(50) values of 5-FC in parent Lovo cells, Lovo cells infected with 100 M.O.I AdCEACD and Lovo cells infected with 10 M.O.I AdCMVCD were 】15000, 216.5+/-38.1 and 128.8+/-25.4 micromol.L(-1), P【0.001, respectively), and the cytotoxicity of 5-FC increased accordingly when the m.o.i of adenoviruses were enhanced (The value of IC(50) of 5-FC was reduced to 27.9+/-4.2 micromol.L(-1) in 1000 M.O.I AdCEACD infected Lovo c展开更多
Accurate determination of the atmospheric particulate matter mass concentration and chemical composition is helpful in exploring the causes and sources of atmospheric enthalpy pollution and in evaluating the rationali...Accurate determination of the atmospheric particulate matter mass concentration and chemical composition is helpful in exploring the causes and sources of atmospheric enthalpy pollution and in evaluating the rationality of environmental air quality control strategies.Based on the sampling and chemical composition data of PM2.5 in different key regions of China in the CARE-China observation network,this research analyzes the environmental air quality data released by the China National Environmental Monitoring Centre during the studied period to determine the changes in the particulate matter mass concentration in key regions and the evolution of the corresponding chemical compositions during the implementation of the Action Plan for Prevention and Control of Air Pollution from 2013-2017.The results show the following.(1)The particulate matter mass concentration in China showed a significant downward trend;however,the PM2.5 annual mass concentration in 64%of cities exceeds the New Chinese Ambient Air Quality Standard(CAAQS)GradeⅡ(GB3095-2012).The region to the east of the Taihang Mountains,the Fenhe and Weihe River Plain and the Urumqi-Changji regions in Xinjiang,all have PM2.5 concentration loading that is still high,and heavy haze pollution occurred frequently in the autumn and winter.(2)During the heavy pollution in the autumn and winter,the concentrations of sulfate and organic components decreased significantly.The mean SO42-concentration in PM2.5 decreased by 76%,12%,81%and 38%in Beijing-Tianjin-Hebei(BTH),the Pearl River Delta(PRD),the Sichuan-Chongqing region(SC)and the Fenhe and Weihe River Plain,respectively.The mean organic matter(OM)concentration decreased by 70%,44%,48%and 31%,respectively,and the mean concentration of NH4+decreased by 68%,1.6%,38%and 25%,respectively.The mean elemental carbon(EC)concentration decreased by 84%and 20%in BTH and SC,respectively,and it increased by 61%and 11%in the PRD and Fenhe and Weihe River Plain,respectively.The mean concentration of mineral and unresolved chemica展开更多
The observation stations of Northern China are divided into three regions:the arid Northwest China,the Loess Plateau,and the cool Northeast China.The consistencies,differences,and associated mechanisms of land surface...The observation stations of Northern China are divided into three regions:the arid Northwest China,the Loess Plateau,and the cool Northeast China.The consistencies,differences,and associated mechanisms of land surface thermal-hydrologic processes among the three regions were studied based on the normalization of major variables of land surface thermal-hydrologic processes,using data collected during prevailing summer monsoon period(July and August,2008).It is shown that differences of surface thermal-hydrologic processes are remarkable among the three regions because of different impacts of summer monsoon.Especially their soil wet layers occur at different depths,and the average albedo and its diurnal variations are distinctly different.Surface net short-wave radiation in the Loess Plateau is close to that in the cool Northeast China,but its surface net long-wave radiation is close to that in the arid Northwest China.And the ratio of net radiation to global solar radiation in the cool Northeast China is higher than the other two regions,though its temperature is lower.There are obvious regional differences in the ratios of surface sensible and latent heat fluxes to net radiation for the three regions because of distinct contribution of sensible and latent heat fluxesto land surface energy balance.The three regions are markedly different in the ratio of water vapor flux to pan evaporation,but they are consistent in the ratio of water vapor flux to precipitation.These results not only indicate different influences of climate and environmental factors on land surface thermal-hydrologic processes in the three regions,but also show that summer monsoon is important in the formation and variation of the pattern of land surface thermal-hydrologic processes.展开更多
We mapped and analyzed the microsatellites throughout 284295605 base pairs ofthe unambiguously assembled sequence scaffolds along 19 chromosomes of the haploid poplargenome.Totally,we found 150985 SSRs with repeat uni...We mapped and analyzed the microsatellites throughout 284295605 base pairs ofthe unambiguously assembled sequence scaffolds along 19 chromosomes of the haploid poplargenome.Totally,we found 150985 SSRs with repeat unit lengths between 2 and 5 bp.The establishedmicrosatellite physical map demonstrated tr at SSRs were distributed relatively evenly across thegenome of Populus.On average,These SSRs occurred every 1883 bp within the poplar genome and the SSRdensities in intergenic regions,introns,exons and UTRs were 85.4%,10.7%,2.7% and1.2%,respectively.We took di-,tri-,tetra-and pentamers as the four classes of repeat units and foundthat the density of each class of SSRs decreased with the repeat unit lengths except for thetetranucleotide repeats.It was noteworthy that the length diversification of microsatellitesequences was negatively correlated with their repeat unit length and the SSRs with shorter repeatunits gained repeats faster than the SSRs with longer repeat units.We also found that the GC contentof poplar sequence significantly correlated with densities of SSRs with uneven repeat unit lengths(tri-and penta-),but had no significant correlation with densities of SSRs with even repeat unitlengths (di-and tetra-).In poplar genome,there were evidences that the occurrence of differentmicrosatellites was under selection and the GC content in SSR sequences was found to significantlyrelate to the functional importance of microsatellites.展开更多
The source regions of the Yangtze and Yellow rivers are important water conservation areas of China. In recent years, ecological deterioration trend of the source regions caused by global climate change and unreasonab...The source regions of the Yangtze and Yellow rivers are important water conservation areas of China. In recent years, ecological deterioration trend of the source regions caused by global climate change and unreasonable resource development increased gradually. In this paper, the spatial distribution and dynamic change of vegetation cover in the source regions of the Yangtze and Yellow rivers are analyzed in recent 10 years based on 1-km resolution multi-temporal SPOTVGT-DN data from 1998 to 2007. Meanwhile, the cor- relation relationships between air temperature, precipitation, shallow ground temperature and NDVI, which is 3x3 pixel at the center of Wudaoliang, Tuotuohe, Qumalai, Maduo, and Dari meteorological stations were analyzed. The results show that the NDVI values in these two source regions are increasing in recent 10 years. Spatial distribution of NDVI which was consistent with hydrothermal condition decreased from southeast to northwest of the source regions. NDVI with a value over 0.54 was mainly distributed in the southeastern source region of the Yellow River, and most NDVI values in the northwestern source region of the Yangtze River were less than 0.22. Spatial changing trend of NDVI has great difference and most parts in the source regions of the Yangtze and Yellow rivers witnessed indistinct change. The regions with marked increasing trend were mainly distributed on the south side of the Tongtian River, some part of Keqianqu, Tongtian, Chumaer, and Tuotuo rivers in the source region of the Yangtze River and Xingsuhai, and southern Dari county in the source region of the Yellow River. The regions with very marked increasing tendency were mainly distributed on the south side of Tongtian Rriver and sporadically distributed in hinterland of the source re- gion of the Yangtze River. The north side of Tangula Range in the source region of the Yangtze River and Dari and Maduo counties in the source region of the Yellow River were areas in which NDVI changed with marked decreasing tendency. The NDVI cha展开更多
文摘Detecting and segmenting salient objects from natural scenes, often referred to as salient object detection, has attracted great interest in computer vision. While many models have been proposed and several applications have emerged, a deep understanding of achievements and issues remains lacking. We aim to provide a comprehensive review of recent progress in salient object detection and situate this field among other closely related areas such as generic scene segmentation, object proposal generation, and saliency for fixation prediction. Covering 228 publications, we survey i) roots, key concepts, and tasks, ii) core techniques and main modeling trends, and iii) datasets and evaluation metrics for salient object detection. We also discuss open problems such as evaluation metrics and dataset bias in model performance, and suggest future research directions.
基金This work was supported by the National Key Research Project (Grant No. 1998040802) the CASKnowledge Innovation Program (Grant No. KZCX2-301), CAREERI Foundation (Grant No. TZ2000-02) and LICCRE Program (Grant No. BX2001-3).
文摘There is no temperature effect in the southern Tibetan Plateau and South Asia to the south of the Tanggula Mountains. Amount effect has been observed at a few sampling stations accounting for about a half of the statistical stations. There is notable temperature effect in the middle and northern Tibetan Plateau and its adjacent Central Asia to the north of the Tanggula Mountains. Because vapor directly originates from low-latitude oceans, the relative heavy ( 18O with small variation characterizes the rainfall in South Asia. A sharp depletion of the stable isotopic compositions in precipitation takes place from Kyangjin on the southern slope of the Himalayas to the Tanggula Mountains in the middle plateau. From the Tanggula Mountains to the northern Tibetan Plateau, the ( 18O in precipitation increases with increasing latitude.
基金Project supported by the China Climbing Project.
文摘The Qinghai-Xizang Plateau is a unique physico-geographical region on the earth. As a whole, the spatial differentiation of physico-geographical regions of the plateau is mainly determined by topographic configuration and atmospheric circulation, warm and humid in the southeast, cold and arid in the northwest. The natural landscapes apppear in the following succession: forest → meadow → steppe → desert. The system of physico-geographical regions of the plateau is demarcated on the principle of bio-climate or the principle of three dimension zonality. Based on the thermal conditions, moisture regimes and variation in landform the Qinghai-Xizang Plateau is sequentially demarcated. The duration of mean daily temperature above 10℃ is the principal index, the subsidiary criterion is mean temperature of the warmest month, two temperature belts may be divided: plateau subpolar and plateau temperate. Annual aridity is taken as the principal index, subordinated by annual precipitation. Four moisture regional types are identified: humid, subhumid, semiarid and arid. A tentative scheme of 2 temperature belts, 10 natural zones and 28 physical districts has been proposed not including southern slopes of the East Himalayas (montane subtropical, humid).
基金Project supported by National Natural Science Foundation of China,No.863 Z2001-04
文摘AIM: To test the hypothesis to block VEGF expression of SMMC-7721 hepatoma cells may inhibit tumor growth using the rat hepatoma model. METHODS: Amplify the 200 VEGF cDNA fragment and insert it into human U6 gene cassette in the reverse orientation transcribing small antisense RNA which could specifically interact with VEGF165, and VEGF121 mRNA. Construct the retroviral vector containing this antisense VEGF U6 cassette and package the replication-deficient recombinant retrovirus. SMMC-7721 cells were transduced with these virus and positive clones were selected with G418. PCR and Southern blot analysis were performed to determine if U6 cassette integrated into the genomic DNA of positive clone. Transfected tumor cells were evaluated for RNA expression by ribonuclease protection assays. The VEGF protein in the supernatant of parental tumor cells and genetically modified tumor cells was determined with ELISA. In vitro and in vivo growth properties of antisense VEGF cell clone in nude mice were analyzed. RESULTS: Restriction enzyme digestion and PCR sequencing verified that the antisense VEGF RNA retroviral vector was successfully constructed.After G418 selection, resistant SMMC-7721 cell clone was picked up. PCR and Southern blot analysis suggested that U6 cassette was integrated into the cell genomic DNA. Stable SMMC-7721 cell clone transduced with U6 antisense RNA cassette could express 200 bp small antisense VEGF RNA and secrete reduced levels of VEGF in culture condition. Production of VEGF by antisense transgene-expressing cells was 65+/-10 ng/L per 10(6) cells, 42045 ng/L per 10(6) cells in sense group and 485+/-30 ng/L per 10(6) cells in the negative control group, (P【 0.05). The antisense-VEGF cell clone appeared phenotypically indistinguishable from SMMC-7721 cells and SMMC-7721 cells transfected sense VEGF. The growth rate of the antisense-VEGF cell clone was the same as the control cells. When S.C. was implanted into nude mice, growth of antisense-VEGF cell lines was greatly inhibited compared with co
基金the Creation Foundation of Nanjing Medical University,No.Cx9905
文摘AIM: To kill CEA positive colorectal carcinoma cells specifically using the E coli cytosine deaminase (CD) suicide gene, a new replication-deficient recombinant adenoviral vector was constructed in which CD gene was controlled under CEA promoter and its in vitro cytotoxic effects were evaluated. METHODS: Shuttle plasmid containing CD gene and regulatory sequence of the CEA gene was constructed and recombined with the right arm of adenovirus genome DNA in 293 cell strain. Dot blotting and PCR were used to identify positive plaques. The purification of adenovirus was performed with ultra-concentration in CsCl step gradients and the titration was measured with plaque formation assay. Cytotoxic effects were assayed with MTT method, The fifty percent inhibition concentration (IC(50)) of 5-FC was calculated using a curve-fitting parameter. The human colorectal carcinoma cell line, which was CEA-producing, and the CEA-nonproducing Hela cell line were applied in cytological tests. An established recombinant adenovirus vector AdCMVCD, in which the CD gene was controlled under CMV promoter, was used as virus control. Quantitative results were expressed as the mean +/- SD of the mean. Statistical analysis was performed using ANOVA test. RESULTS: The desired recombinant adenovirus vector was named AdCEACD. The results of dot blotting and PCR showed that the recombinant adenovirus contained CEA promoter and CD gene. Virus titer was about 5.0 X 10(14)pfu/L(-1) after purification. The CEA-producing Lovo cells were sensitive to 5-FC and had the same cytotoxic effect after infection with AdCEACD and AdCMVCD (The IC(50) values of 5-FC in parent Lovo cells, Lovo cells infected with 100 M.O.I AdCEACD and Lovo cells infected with 10 M.O.I AdCMVCD were 】15000, 216.5+/-38.1 and 128.8+/-25.4 micromol.L(-1), P【0.001, respectively), and the cytotoxicity of 5-FC increased accordingly when the m.o.i of adenoviruses were enhanced (The value of IC(50) of 5-FC was reduced to 27.9+/-4.2 micromol.L(-1) in 1000 M.O.I AdCEACD infected Lovo c
基金supported by the Ministry of Science and Technology National Key Research and Development Program (Grant No. 2017YFC0210000)the Fundamental Heavy Pollution Cause and Governance Research Project (Grant No. DQGG0101)the Beijing Municipal Science and Technology Commission Capital Blue Sky Action and Cultivation Project (Grant No. Z181100005418014)
文摘Accurate determination of the atmospheric particulate matter mass concentration and chemical composition is helpful in exploring the causes and sources of atmospheric enthalpy pollution and in evaluating the rationality of environmental air quality control strategies.Based on the sampling and chemical composition data of PM2.5 in different key regions of China in the CARE-China observation network,this research analyzes the environmental air quality data released by the China National Environmental Monitoring Centre during the studied period to determine the changes in the particulate matter mass concentration in key regions and the evolution of the corresponding chemical compositions during the implementation of the Action Plan for Prevention and Control of Air Pollution from 2013-2017.The results show the following.(1)The particulate matter mass concentration in China showed a significant downward trend;however,the PM2.5 annual mass concentration in 64%of cities exceeds the New Chinese Ambient Air Quality Standard(CAAQS)GradeⅡ(GB3095-2012).The region to the east of the Taihang Mountains,the Fenhe and Weihe River Plain and the Urumqi-Changji regions in Xinjiang,all have PM2.5 concentration loading that is still high,and heavy haze pollution occurred frequently in the autumn and winter.(2)During the heavy pollution in the autumn and winter,the concentrations of sulfate and organic components decreased significantly.The mean SO42-concentration in PM2.5 decreased by 76%,12%,81%and 38%in Beijing-Tianjin-Hebei(BTH),the Pearl River Delta(PRD),the Sichuan-Chongqing region(SC)and the Fenhe and Weihe River Plain,respectively.The mean organic matter(OM)concentration decreased by 70%,44%,48%and 31%,respectively,and the mean concentration of NH4+decreased by 68%,1.6%,38%and 25%,respectively.The mean elemental carbon(EC)concentration decreased by 84%and 20%in BTH and SC,respectively,and it increased by 61%and 11%in the PRD and Fenhe and Weihe River Plain,respectively.The mean concentration of mineral and unresolved chemica
基金supported by State Key Program of National Natural Science Foundation of China (Grant No. 40830957)Public Welfare Research Project of China (Grant No. GYHY200806021)
文摘The observation stations of Northern China are divided into three regions:the arid Northwest China,the Loess Plateau,and the cool Northeast China.The consistencies,differences,and associated mechanisms of land surface thermal-hydrologic processes among the three regions were studied based on the normalization of major variables of land surface thermal-hydrologic processes,using data collected during prevailing summer monsoon period(July and August,2008).It is shown that differences of surface thermal-hydrologic processes are remarkable among the three regions because of different impacts of summer monsoon.Especially their soil wet layers occur at different depths,and the average albedo and its diurnal variations are distinctly different.Surface net short-wave radiation in the Loess Plateau is close to that in the cool Northeast China,but its surface net long-wave radiation is close to that in the arid Northwest China.And the ratio of net radiation to global solar radiation in the cool Northeast China is higher than the other two regions,though its temperature is lower.There are obvious regional differences in the ratios of surface sensible and latent heat fluxes to net radiation for the three regions because of distinct contribution of sensible and latent heat fluxesto land surface energy balance.The three regions are markedly different in the ratio of water vapor flux to pan evaporation,but they are consistent in the ratio of water vapor flux to precipitation.These results not only indicate different influences of climate and environmental factors on land surface thermal-hydrologic processes in the three regions,but also show that summer monsoon is important in the formation and variation of the pattern of land surface thermal-hydrologic processes.
基金Supported by Program for New Century Excellent Talents in University (Grant No. NCET-04-0516)Fok Ying Tung Education Foundation, and the National Natural Science Foundation of China (Grant No. 30200224)
文摘We mapped and analyzed the microsatellites throughout 284295605 base pairs ofthe unambiguously assembled sequence scaffolds along 19 chromosomes of the haploid poplargenome.Totally,we found 150985 SSRs with repeat unit lengths between 2 and 5 bp.The establishedmicrosatellite physical map demonstrated tr at SSRs were distributed relatively evenly across thegenome of Populus.On average,These SSRs occurred every 1883 bp within the poplar genome and the SSRdensities in intergenic regions,introns,exons and UTRs were 85.4%,10.7%,2.7% and1.2%,respectively.We took di-,tri-,tetra-and pentamers as the four classes of repeat units and foundthat the density of each class of SSRs decreased with the repeat unit lengths except for thetetranucleotide repeats.It was noteworthy that the length diversification of microsatellitesequences was negatively correlated with their repeat unit length and the SSRs with shorter repeatunits gained repeats faster than the SSRs with longer repeat units.We also found that the GC contentof poplar sequence significantly correlated with densities of SSRs with uneven repeat unit lengths(tri-and penta-),but had no significant correlation with densities of SSRs with even repeat unitlengths (di-and tetra-).In poplar genome,there were evidences that the occurrence of differentmicrosatellites was under selection and the GC content in SSR sequences was found to significantlyrelate to the functional importance of microsatellites.
基金National Basic Task Project, No.2006FY110200Strategic pilot programs of the Chinese Academy of Sciences,No.XDA05060700Ministry of Environmental Protection Special Funds for Scientific Research on Public Causes, No.200909050
文摘The source regions of the Yangtze and Yellow rivers are important water conservation areas of China. In recent years, ecological deterioration trend of the source regions caused by global climate change and unreasonable resource development increased gradually. In this paper, the spatial distribution and dynamic change of vegetation cover in the source regions of the Yangtze and Yellow rivers are analyzed in recent 10 years based on 1-km resolution multi-temporal SPOTVGT-DN data from 1998 to 2007. Meanwhile, the cor- relation relationships between air temperature, precipitation, shallow ground temperature and NDVI, which is 3x3 pixel at the center of Wudaoliang, Tuotuohe, Qumalai, Maduo, and Dari meteorological stations were analyzed. The results show that the NDVI values in these two source regions are increasing in recent 10 years. Spatial distribution of NDVI which was consistent with hydrothermal condition decreased from southeast to northwest of the source regions. NDVI with a value over 0.54 was mainly distributed in the southeastern source region of the Yellow River, and most NDVI values in the northwestern source region of the Yangtze River were less than 0.22. Spatial changing trend of NDVI has great difference and most parts in the source regions of the Yangtze and Yellow rivers witnessed indistinct change. The regions with marked increasing trend were mainly distributed on the south side of the Tongtian River, some part of Keqianqu, Tongtian, Chumaer, and Tuotuo rivers in the source region of the Yangtze River and Xingsuhai, and southern Dari county in the source region of the Yellow River. The regions with very marked increasing tendency were mainly distributed on the south side of Tongtian Rriver and sporadically distributed in hinterland of the source re- gion of the Yangtze River. The north side of Tangula Range in the source region of the Yangtze River and Dari and Maduo counties in the source region of the Yellow River were areas in which NDVI changed with marked decreasing tendency. The NDVI cha